Drosophila Dok is required for embryonic dorsal closure

Romi Biswas, David Stein, E. Richard Stanley

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Embryonic dorsal closure (DC) in Drosophila is a series of morphogenetic movements involving the bilateral dorsal movement of the epidermis (cell stretching) and dorsal suturing of the leading edge (LE) cells to enclose the viscera. The Syk family tyrosine kinase Shark plays a crucial role in this Jun amino-terminal kinase (JNK)-dependent process, where it acts upstream of JNK in LE cells. Using a yeast two-hybrid screen, the unique Drosophila homolog of the downstream of kinase (Dok) family, Ddok, was identified by its ability to bind Shark SH2 domains in a tyrosine phosphorylation-dependent fashion. In cultured S2 embryonic cells, Ddok tyrosine phosphorylation is Src dependent; Shark associates with Ddok and Ddok localizes at the cell cortex, together with a portion of the Shark protein. The embryonic expression pattern of Ddok resembles the expression pattern of Shark. Ddok loss-of-function mutant (DdokPG155) germ-line clones possess DC defects, including the loss of JNK-dependent expression of dpp mRNA in LE cells, and decreased epidermal F-actin staining and LE actin cable formation. Epistatic analysis indicates that Ddok functions upstream of shark to activate JNK signaling during DC. Consistent with these observations, Ddok mutant embryos exhibit decreased levels of tyrosine phosphorylated Shark at the cell periphery of LE and epidermal cells. As there are six mammalian Dok family members that exhibit some functional redundancy, analysis of the regulation of DC by Ddok is expected to provide novel insights into the function of the Dok adapter proteins.

Original languageEnglish (US)
Pages (from-to)217-227
Number of pages11
JournalDevelopment
Volume133
Issue number2
DOIs
Publication statusPublished - Jan 1 2006

    Fingerprint

Keywords

  • Dok
  • Dorsal closure
  • Drosophila embryogenesis
  • Jun kinase
  • Morphogenesis
  • Shark tyrosine kinase

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

Cite this

Biswas, R., Stein, D., & Stanley, E. R. (2006). Drosophila Dok is required for embryonic dorsal closure. Development, 133(2), 217-227. https://doi.org/10.1242/dev.02198