Downregulation of enkephalin-mediated inflammatory responses by CD10/neutral endopeptidase 24.11

Margaret A. Shipp, George B. Stefano, Luciano D'Adamio, Stephanie N. Switzer, Frank D. Howard, Juan Sinisterra, Berta Scharrer, Ellis L. Reinherz

Research output: Contribution to journalArticle

157 Citations (Scopus)

Abstract

THE antigen CD10 (common acute lymphoblastic leukaemia antigen), which is the zinc metalloprotease, neutral endopeptidase 24.11 (also known as NEP or 'enkephalinase')1-7, is expressed by acute lymphoblastic leukaemias8,9, normal lymphoid progenitors10, mature polymorphonuclear leukocytes11 and certain non-haematopoietic cells12. CD10/NEP hydrolyses several naturally occurring peptides, including the endogenous opioid pentapeptides Met- and Leu-enkephalin6. In invertebrate organisms such as the mollusc Mytilus edulis, Met-enkephalin triggers inflammatory responses by inducing morphological changes, directed migration and aggregation of haemocytes13,14. We report here that a structure related to CD10/NEP is expressed by M. edulis haemocytes and that abrogation of CD10/NEP enzymatic activity reduces the amount of Met-enkephalin required for haemocyte activation by five orders of magnitude. Similar results are obtained with CD10+ human polymorphonuclear leukocytes, indicating that CD10/NEP related structures regulate enkephalin-mediated inflammatory responses in organisms whose ancestors diverged ∼500 million years ago15.

Original languageEnglish (US)
Pages (from-to)394-396
Number of pages3
JournalNature
Volume347
Issue number6291
StatePublished - Sep 27 1990
Externally publishedYes

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Neprilysin
Enkephalins
Down-Regulation
Mytilus edulis
Hemocytes
Methionine Enkephalin
Mollusca
Metalloproteases
Invertebrates
Opioid Analgesics
Zinc
Neutrophils
Peptides

ASJC Scopus subject areas

  • General

Cite this

Shipp, M. A., Stefano, G. B., D'Adamio, L., Switzer, S. N., Howard, F. D., Sinisterra, J., ... Reinherz, E. L. (1990). Downregulation of enkephalin-mediated inflammatory responses by CD10/neutral endopeptidase 24.11. Nature, 347(6291), 394-396.

Downregulation of enkephalin-mediated inflammatory responses by CD10/neutral endopeptidase 24.11. / Shipp, Margaret A.; Stefano, George B.; D'Adamio, Luciano; Switzer, Stephanie N.; Howard, Frank D.; Sinisterra, Juan; Scharrer, Berta; Reinherz, Ellis L.

In: Nature, Vol. 347, No. 6291, 27.09.1990, p. 394-396.

Research output: Contribution to journalArticle

Shipp, MA, Stefano, GB, D'Adamio, L, Switzer, SN, Howard, FD, Sinisterra, J, Scharrer, B & Reinherz, EL 1990, 'Downregulation of enkephalin-mediated inflammatory responses by CD10/neutral endopeptidase 24.11', Nature, vol. 347, no. 6291, pp. 394-396.
Shipp MA, Stefano GB, D'Adamio L, Switzer SN, Howard FD, Sinisterra J et al. Downregulation of enkephalin-mediated inflammatory responses by CD10/neutral endopeptidase 24.11. Nature. 1990 Sep 27;347(6291):394-396.
Shipp, Margaret A. ; Stefano, George B. ; D'Adamio, Luciano ; Switzer, Stephanie N. ; Howard, Frank D. ; Sinisterra, Juan ; Scharrer, Berta ; Reinherz, Ellis L. / Downregulation of enkephalin-mediated inflammatory responses by CD10/neutral endopeptidase 24.11. In: Nature. 1990 ; Vol. 347, No. 6291. pp. 394-396.
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