Down-regulation of NF-κB signalling by polyphenolic compounds prevents endotoxin-induced liver injury in a rat model

Sushma Bharrhan, Kanwaljit Chopra, Sunil K. Arora, Jaideep S. Toor, Praveen Rishi

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Activation of NF-κB has been reported to play a key role in causing endotoxin-induced hepatic damage through enhanced production of reactive oxygen species and pro-inflammatory mediators. In this context, the potential of polyphenolic phytochemicals in preventing endotoxin-induced liver damage remains unclear. Here, we demonstrate that catechin and quercetin have the potential to down-regulate the initial signalling molecule NF-κB which may further inhibit the downstream cascade including TNF-α and NO. These results were confirmed using N-nitro-L-arginine methyl ester (L-NAME), the inhibitor of inducible nitric oxide synthase (iNOS) along with the biochemical and histological alterations occurring in the presence and absence of supplementation with both the polyphenols. However, catechin was found to be more effective than quercetin against endotoxin-induced liver injury. These findings suggest that these polyphenols may form a pharmacological basis for designing a therapeutic agent against endotoxin-mediated oxidative damage.

Original languageEnglish (US)
Pages (from-to)70-79
Number of pages10
JournalInnate Immunity
Volume18
Issue number1
DOIs
StatePublished - Feb 1 2012
Externally publishedYes

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Keywords

  • Endotoxin
  • liver injury
  • nuclear factor kappa B (NF-κB)
  • polyphenols
  • tumor necrosis factor alpha (TNF-α)

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Molecular Biology
  • Cell Biology
  • Infectious Diseases

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