Down regulation of monocyte antigen presentation in critical illness and septic shock

Janice Manjuck, Dhanonjoy C. Saha, Mark Astiz, Jane Eales, Eric Rackow

Research output: Contribution to journalArticle

Abstract

Introduction: Monocyte HLA-DR expression is decreased in sepsis. However, antigen presentation and the expression of other costimulatory molecules have not been studied in this setting. Methods: We examined antigen presentation (Antig P) using in vitro lymphocyte response to tuberculin toxoid. Lymphocyte incorporation of 5-bromo-2-deoxyuridine was measured by ELISA as % absorbance (% abs). Ten normal controls, 10 critically ill non-septic patients (CINS), 15 patients with septic shock (SS) were studied. Monocyte (M) HLA-DR and CD-86 receptors was evaluated by flow cytometry as were lymphocyte (L) expression of CD28 and CTLA. Flow cytometry data are presented as % expression. All data are presented as mean ± SE.*P

Original languageEnglish (US)
JournalCritical Care Medicine
Volume27
Issue number1 SUPPL.
StatePublished - 1999
Externally publishedYes

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Antigen Presentation
Septic Shock
Critical Illness
Monocytes
Down-Regulation
HLA-DR Antigens
Lymphocytes
Flow Cytometry
Toxoids
Tuberculin
Bromodeoxyuridine
Sepsis
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

Cite this

Down regulation of monocyte antigen presentation in critical illness and septic shock. / Manjuck, Janice; Saha, Dhanonjoy C.; Astiz, Mark; Eales, Jane; Rackow, Eric.

In: Critical Care Medicine, Vol. 27, No. 1 SUPPL., 1999.

Research output: Contribution to journalArticle

Manjuck, Janice ; Saha, Dhanonjoy C. ; Astiz, Mark ; Eales, Jane ; Rackow, Eric. / Down regulation of monocyte antigen presentation in critical illness and septic shock. In: Critical Care Medicine. 1999 ; Vol. 27, No. 1 SUPPL.
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