Abstract
Introduction: Monocyte HLA-DR expression is decreased in sepsis. However, antigen presentation and the expression of other costimulatory molecules have not been studied in this setting. Methods: We examined antigen presentation (Antig P) using in vitro lymphocyte response to tuberculin toxoid. Lymphocyte incorporation of 5-bromo-2-deoxyuridine was measured by ELISA as % absorbance (% abs). Ten normal controls, 10 critically ill non-septic patients (CINS), 15 patients with septic shock (SS) were studied. Monocyte (M) HLA-DR and CD-86 receptors was evaluated by flow cytometry as were lymphocyte (L) expression of CD28 and CTLA. Flow cytometry data are presented as % expression. All data are presented as mean ± SE.*P
| Original language | English (US) |
|---|---|
| Journal | Critical Care Medicine |
| Volume | 27 |
| Issue number | 1 SUPPL. |
| State | Published - 1999 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Critical Care and Intensive Care Medicine
Cite this
Down regulation of monocyte antigen presentation in critical illness and septic shock. / Manjuck, Janice; Saha, Dhanonjoy C.; Astiz, Mark; Eales, Jane; Rackow, Eric.
In: Critical Care Medicine, Vol. 27, No. 1 SUPPL., 1999.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Down regulation of monocyte antigen presentation in critical illness and septic shock
AU - Manjuck, Janice
AU - Saha, Dhanonjoy C.
AU - Astiz, Mark
AU - Eales, Jane
AU - Rackow, Eric
PY - 1999
Y1 - 1999
N2 - Introduction: Monocyte HLA-DR expression is decreased in sepsis. However, antigen presentation and the expression of other costimulatory molecules have not been studied in this setting. Methods: We examined antigen presentation (Antig P) using in vitro lymphocyte response to tuberculin toxoid. Lymphocyte incorporation of 5-bromo-2-deoxyuridine was measured by ELISA as % absorbance (% abs). Ten normal controls, 10 critically ill non-septic patients (CINS), 15 patients with septic shock (SS) were studied. Monocyte (M) HLA-DR and CD-86 receptors was evaluated by flow cytometry as were lymphocyte (L) expression of CD28 and CTLA. Flow cytometry data are presented as % expression. All data are presented as mean ± SE.*P
AB - Introduction: Monocyte HLA-DR expression is decreased in sepsis. However, antigen presentation and the expression of other costimulatory molecules have not been studied in this setting. Methods: We examined antigen presentation (Antig P) using in vitro lymphocyte response to tuberculin toxoid. Lymphocyte incorporation of 5-bromo-2-deoxyuridine was measured by ELISA as % absorbance (% abs). Ten normal controls, 10 critically ill non-septic patients (CINS), 15 patients with septic shock (SS) were studied. Monocyte (M) HLA-DR and CD-86 receptors was evaluated by flow cytometry as were lymphocyte (L) expression of CD28 and CTLA. Flow cytometry data are presented as % expression. All data are presented as mean ± SE.*P
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M3 - Article
VL - 27
JO - Critical Care Medicine
JF - Critical Care Medicine
SN - 0090-3493
IS - 1 SUPPL.
ER -