Dopamine receptors on human T- and B-lymphocytes

Laura Santambrogio, Maria Lipartiti, Alessandro Bruni, Roberto Dal Toso

Research output: Contribution to journalArticle

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Abstract

The presence of functional dopamine receptors on differentiated cells of the mammalian immune system is still under discussion. This study has utilized (-)-[3H]sulpride as a ligand to detect the presence of recognition sites of the dopamine D2 receptor family on human T- and B-lymphocytes. The (-)-[3H]sulpiride binding was of high affinity (Kd 0.9 nM ± 0.2 nM, specific, saturable (Bmax 10.2 ± 1.4 fmol/106 cells) and reversible. The pharmacological characterization of the recognition site suggests, similarities mainly with the D2 and D4 rather than D3 subtype of dopamine receptor. Furthermore, dopamine treatment was able to reduce the intracellular cAMP levels of lymphocytes stimulated with forskolin, thus suggesting a potential functional significance of this dopamine receptor in mediating neural-immune interactions.

Original languageEnglish (US)
Pages (from-to)113-119
Number of pages7
JournalJournal of Neuroimmunology
Volume45
Issue number1-2
DOIs
StatePublished - Jun 1993

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Keywords

  • Dopamine receptor
  • Human lymphocyte
  • Neuroimmunomodulation
  • Sulpiride
  • cAMP

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

Cite this

Santambrogio, L., Lipartiti, M., Bruni, A., & Toso, R. D. (1993). Dopamine receptors on human T- and B-lymphocytes. Journal of Neuroimmunology, 45(1-2), 113-119. https://doi.org/10.1016/0165-5728(93)90170-4