Domain-Specific Effector Interactions within the Central Cavity of Human Adult Hemoglobin in Solution and in Porous Sol-Gel Matrices: Evidence for Long-Range Communication Pathways

Eric S. Peterson, Roman Shinder, Imran Khan, Laura Juczszak, Jiaqian Wang, Belur Manjula, Seetharama A. Acharya, Celia Bonaventura, Joel M. Friedman

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The water-filled central cavity of human adult hemoglobin (Hb A) is the binding or interaction site for many different allosteric effectors. Oxygen binding titrations reveal that pyrenetetrasulfonate (PyTS), a fluorescent analogue of 2,3-diphosphoglycerate, behaves like an allosteric effector. The ligation state, pH, and concentrations of other effectors (IHP, L35, and chloride) alter PyTS fluorescence for both solution-phase and sol-gel-encapsulated Hb samples. These conditions also alter the resonance Raman spectra and rates of geminate recombination of CO-ligated Hb. Together, these results demonstrate that there are conformational and functional consequences resulting from interactions between specific domains of the central cavity and individual effectors as well as from long-range synergistic effects that are mediated through the central cavity.

Original languageEnglish (US)
Pages (from-to)4832-4843
Number of pages12
JournalBiochemistry
Volume43
Issue number16
DOIs
StatePublished - Apr 27 2004

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L 35
2,3-Diphosphoglycerate
Polymethyl Methacrylate
Carbon Monoxide
Titration
Genetic Recombination
Sol-gels
Ligation
Raman scattering
Chlorides
Hemoglobins
Fluorescence
Gels
Communication
Oxygen
Water

ASJC Scopus subject areas

  • Biochemistry

Cite this

Domain-Specific Effector Interactions within the Central Cavity of Human Adult Hemoglobin in Solution and in Porous Sol-Gel Matrices : Evidence for Long-Range Communication Pathways. / Peterson, Eric S.; Shinder, Roman; Khan, Imran; Juczszak, Laura; Wang, Jiaqian; Manjula, Belur; Acharya, Seetharama A.; Bonaventura, Celia; Friedman, Joel M.

In: Biochemistry, Vol. 43, No. 16, 27.04.2004, p. 4832-4843.

Research output: Contribution to journalArticle

Peterson, Eric S. ; Shinder, Roman ; Khan, Imran ; Juczszak, Laura ; Wang, Jiaqian ; Manjula, Belur ; Acharya, Seetharama A. ; Bonaventura, Celia ; Friedman, Joel M. / Domain-Specific Effector Interactions within the Central Cavity of Human Adult Hemoglobin in Solution and in Porous Sol-Gel Matrices : Evidence for Long-Range Communication Pathways. In: Biochemistry. 2004 ; Vol. 43, No. 16. pp. 4832-4843.
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