Does the method of Helicobacter pylori detection influence the association with gastric cancer risk?

Helena Enroth, W. Kraaz, Thomas E. Rohan, O. Nyrén, L. Engstrand

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Background: The exact role of Helicobacter pylori as a causative agent of gastric cancer is still under debate. The aim of this study was to determine how the use of different diagnostic methods for detection of H. pylori influences the measures of prevalence of the infection and thus the association with risk of gastric adenocarcinoma. Methods: We included 72 cases and 324 controls in an endoscopy clinic-based matched case-control study. Culture of H. pylori and immunohistochemical staining were performed on gastric biopsies. Serum samples were tested for H. pylori IgG by conventional ELISA and by immunoblotting. Results: The overall prevalence of H. pylori was 68% based on all 4 diagnostic methods, 79% in the cases and 66% in the controls. Highest agreement, 91%, was observed between culture and immunohistochemistry with a Kappa value of 0.81. Immunoblotting detected the highest number of H. pylori-positive subjects in both cases and controls. The association of H. pylori positivity with gastric cancer was generally weaker and statistically non-significant using culture and immunohistochemistry compared with the serological tests, of which IgG ELISA yielded the higher odds ratio (OR 2.5, 95% confidence interval 1.4-4.4). Conclusion: The study shows that relative risk estimates for the association between H. pylori and gastric cancer risk are to some extent determined by the diagnostic method used to detect H. pylori infection.

Original languageEnglish (US)
Pages (from-to)884-890
Number of pages7
JournalScandinavian Journal of Gastroenterology
Volume37
Issue number8
DOIs
StatePublished - Aug 2002
Externally publishedYes

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Helicobacter pylori
Stomach Neoplasms
Immunoblotting
Stomach
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry
Helicobacter Infections
Serologic Tests
Endoscopy
Case-Control Studies
Adenocarcinoma
Odds Ratio
Confidence Intervals
Staining and Labeling
Biopsy
Infection
Serum

Keywords

  • Cancer risk
  • Case-control
  • Culture
  • ELISA
  • Gastric cancer
  • Helicobacter pylori
  • Immunoblotting
  • Immunohistochemistry
  • Prevalence
  • Serology

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Does the method of Helicobacter pylori detection influence the association with gastric cancer risk? / Enroth, Helena; Kraaz, W.; Rohan, Thomas E.; Nyrén, O.; Engstrand, L.

In: Scandinavian Journal of Gastroenterology, Vol. 37, No. 8, 08.2002, p. 884-890.

Research output: Contribution to journalArticle

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