DNA structure and flexibility in the sequence-specific binding of papillomavirus E2 proteins

Christina S. Hines, Colin Meghoo, Sanjay Shetty, Markus Biburger, Michael D. Brenowitz, Rashmi S. Hegde

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

The papillomavirus E2 proteins are transcriptional regulators that bind to a consensus DNA sequence ACCG NNNN CGGT. Multiple copies of this binding site are found in the viral genomes. The affinities of the naturally occurring binding sites for the E2 proteins are predominantly dependent upon the sequence of the NNNN spacer. The hierarchies of binding site affinities among the sites present in the viral genomes result in differential occupancy during the viral life-cycle. In turn, this differential binding regulates transcription from viral promoters, including those for the oncogenes E6 and E7. Structural and biochemical studies have shown that E2 proteins bend the DNA to which they specifically bind. Atomic resolution structures of complexes of the bovine papillomavirus strain 1 (BPV-1) E2 protein and DNA show that the protein does not contact the spacer DNA. A direct comparison of the binding of the DNA-binding domains of the E2 proteins from BPV-1 and human papillomavirus strain 16 (HPV-16) to a series of binding sites as a function of the sequence of their central spacer and/or the presence of a nick or gap in one strand of the spacer DNA is presented in this paper. The BPV-1 E2 DNA-binding domain is only moderately sensitive to the nature of the central spacer; less than several fold differences in affinity were observed when the DNA sequence of the spacer was varied and/or a nick or gap was introduced. In contrast, the HPV-16 E2 DNA-binding domain binds to sites containing A:T-rich central spacers with significantly increased affinity. The introduction of a nick or gap into the spacer of these high affinity sequences is very detrimental to HPV-16 E2 binding while comparable nicks or gaps have only small effects in the low affinity sequences. These results suggest that the HPV-16 E2 protein recognizes the structure of the DNA spacer and that the mechanism of DNA-sequence specific binding of the homologous HPV-16 E2 and BPV-1 E2 proteins is significantly different.

Original languageEnglish (US)
Pages (from-to)809-818
Number of pages10
JournalJournal of Molecular Biology
Volume276
Issue number4
DOIs
StatePublished - Mar 6 1998
Externally publishedYes

Fingerprint

Bovine papillomavirus 1
Human papillomavirus 16
Intergenic DNA
DNA
Binding Sites
Proteins
Viral Genome
Consensus Sequence
Life Cycle Stages
Oncogenes

Keywords

  • DNA conformation
  • DNA flexibility
  • DNA-binding
  • E2
  • Papillomavirus

ASJC Scopus subject areas

  • Virology

Cite this

DNA structure and flexibility in the sequence-specific binding of papillomavirus E2 proteins. / Hines, Christina S.; Meghoo, Colin; Shetty, Sanjay; Biburger, Markus; Brenowitz, Michael D.; Hegde, Rashmi S.

In: Journal of Molecular Biology, Vol. 276, No. 4, 06.03.1998, p. 809-818.

Research output: Contribution to journalArticle

Hines, Christina S. ; Meghoo, Colin ; Shetty, Sanjay ; Biburger, Markus ; Brenowitz, Michael D. ; Hegde, Rashmi S. / DNA structure and flexibility in the sequence-specific binding of papillomavirus E2 proteins. In: Journal of Molecular Biology. 1998 ; Vol. 276, No. 4. pp. 809-818.
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