DNA sequences 3′ of the Ig H chain cluster rearrange in mouse B cell lines

Charles Félix Calvo, Sandra L. Giannini, Nancy Martinez, Barbara K. Birshtein

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A mouse myeloma cell line MPC11 (IgG2b, κ) and variants derived from it have been used to study DNA rearrangements that occur at the Ig H chain locus. One variant, F5.5, has acquired both VH gene and Cε gene rearrangements. Through genomic Southern blot analysis initially directed to mapping the Cε gene rearrangement, we observed that the VH region rearrangement was linked, through an inversion event, to sequences that originate 3′ of the CH cluster, i.e., 3′ of the Cα gene. Subsequent studies have shown that DNA rearrangements within the region 3′ of the Ca gene are detected in several other mouse myeloma and hybridoma cell lines and are not associated with the expression of specific isotypes.

Original languageEnglish (US)
Pages (from-to)1353-1360
Number of pages8
JournalJournal of Immunology
Volume146
Issue number4
StatePublished - Feb 15 1991

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Gene Rearrangement
B-Lymphocytes
Cell Line
Genes
Hybridomas
Southern Blotting

ASJC Scopus subject areas

  • Immunology

Cite this

Calvo, C. F., Giannini, S. L., Martinez, N., & Birshtein, B. K. (1991). DNA sequences 3′ of the Ig H chain cluster rearrange in mouse B cell lines. Journal of Immunology, 146(4), 1353-1360.

DNA sequences 3′ of the Ig H chain cluster rearrange in mouse B cell lines. / Calvo, Charles Félix; Giannini, Sandra L.; Martinez, Nancy; Birshtein, Barbara K.

In: Journal of Immunology, Vol. 146, No. 4, 15.02.1991, p. 1353-1360.

Research output: Contribution to journalArticle

Calvo, CF, Giannini, SL, Martinez, N & Birshtein, BK 1991, 'DNA sequences 3′ of the Ig H chain cluster rearrange in mouse B cell lines', Journal of Immunology, vol. 146, no. 4, pp. 1353-1360.
Calvo CF, Giannini SL, Martinez N, Birshtein BK. DNA sequences 3′ of the Ig H chain cluster rearrange in mouse B cell lines. Journal of Immunology. 1991 Feb 15;146(4):1353-1360.
Calvo, Charles Félix ; Giannini, Sandra L. ; Martinez, Nancy ; Birshtein, Barbara K. / DNA sequences 3′ of the Ig H chain cluster rearrange in mouse B cell lines. In: Journal of Immunology. 1991 ; Vol. 146, No. 4. pp. 1353-1360.
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