A mouse myeloma cell line MPC11 (IgG2b, K) and variants derived from it have been used to study DNA rearrangements that occur at the Ig H chain locus. One variant, F5.5, has acquired both V(H) gene and Cε gene rearrangements. Through genomic Southern blot analysis initially directed to mapping the Cε gene rearrangement, we observed that the V(H) region rearrangement was linked, through an inversion event, to sequences that originate 3' of the C(H) cluster, i.e., 3' of the Cα gene. Subsequent studies have shown that DNA rearrangements within the region 3' of the Cα gene are detected in several other mouse myeloma and hybridoma cell lines and are not associated with the expression of specific isotypes.
|Original language||English (US)|
|Number of pages||8|
|Journal||Journal of Immunology|
|State||Published - 1991|
ASJC Scopus subject areas
- Immunology and Allergy