TY - JOUR
T1 - DNA sequences 3′ of the Ig H chain cluster rearrange in mouse B cell lines
AU - Calvo, Charles Félix
AU - Giannini, Sandra L.
AU - Martinez, Nancy
AU - Birshtein, Barbara K.
PY - 1991/2/15
Y1 - 1991/2/15
N2 - A mouse myeloma cell line MPC11 (IgG2b, κ) and variants derived from it have been used to study DNA rearrangements that occur at the Ig H chain locus. One variant, F5.5, has acquired both VH gene and Cε gene rearrangements. Through genomic Southern blot analysis initially directed to mapping the Cε gene rearrangement, we observed that the VH region rearrangement was linked, through an inversion event, to sequences that originate 3′ of the CH cluster, i.e., 3′ of the Cα gene. Subsequent studies have shown that DNA rearrangements within the region 3′ of the Ca gene are detected in several other mouse myeloma and hybridoma cell lines and are not associated with the expression of specific isotypes.
AB - A mouse myeloma cell line MPC11 (IgG2b, κ) and variants derived from it have been used to study DNA rearrangements that occur at the Ig H chain locus. One variant, F5.5, has acquired both VH gene and Cε gene rearrangements. Through genomic Southern blot analysis initially directed to mapping the Cε gene rearrangement, we observed that the VH region rearrangement was linked, through an inversion event, to sequences that originate 3′ of the CH cluster, i.e., 3′ of the Cα gene. Subsequent studies have shown that DNA rearrangements within the region 3′ of the Ca gene are detected in several other mouse myeloma and hybridoma cell lines and are not associated with the expression of specific isotypes.
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M3 - Article
C2 - 1991972
AN - SCOPUS:0025982149
SN - 0022-1767
VL - 146
SP - 1353
EP - 1360
JO - Journal of Immunology
JF - Journal of Immunology
IS - 4
ER -