DNA replication in synchronized cultured mammalian cells. VI. The temporal replication of ribosomal cistrons in synchronized cell lines

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Abstract

The time of synthesis of ribosomal genes was studied in a haploid (Rana pipiens), and a pseudodiploid (Chinese hamster) cell line. R. pipiens cells were synchronized by amethopterin block. Chinese hamster cells were synchronized by isoleucine starvation followed by hydroxyurea treatment. DNA replicated during three or four selected intervals of the S period was separated from the remainder of the DNA by bromodeoxyuridine density labeling. Purified bromodeoxyuridine substituted DNA was annealed with radioactive-labeled 28S ribosomal RNA (rRNA) to determine when, during different intervals of S, the nuclear DNA homologous to rRNA was replicated. In the R. pipiens and Chinese hamster cell lines, the percent of nuclear DNA homologous to 28S rRNA is highest in the DNA replicated during the first half of the S period.

Original languageEnglish (US)
Pages (from-to)307-314
Number of pages8
JournalExperimental Cell Research
Volume101
Issue number2
DOIs
StatePublished - 1976

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DNA Replication
Cultured Cells
Rana pipiens
Cell Line
DNA
Cricetulus
Genes
Bromodeoxyuridine
S Phase
28S Ribosomal RNA
Ribosomal RNA
Hydroxyurea
Isoleucine
Haploidy
Starvation
Methotrexate

ASJC Scopus subject areas

  • Cell Biology

Cite this

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title = "DNA replication in synchronized cultured mammalian cells. VI. The temporal replication of ribosomal cistrons in synchronized cell lines",
abstract = "The time of synthesis of ribosomal genes was studied in a haploid (Rana pipiens), and a pseudodiploid (Chinese hamster) cell line. R. pipiens cells were synchronized by amethopterin block. Chinese hamster cells were synchronized by isoleucine starvation followed by hydroxyurea treatment. DNA replicated during three or four selected intervals of the S period was separated from the remainder of the DNA by bromodeoxyuridine density labeling. Purified bromodeoxyuridine substituted DNA was annealed with radioactive-labeled 28S ribosomal RNA (rRNA) to determine when, during different intervals of S, the nuclear DNA homologous to rRNA was replicated. In the R. pipiens and Chinese hamster cell lines, the percent of nuclear DNA homologous to 28S rRNA is highest in the DNA replicated during the first half of the S period.",
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year = "1976",
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T1 - DNA replication in synchronized cultured mammalian cells. VI. The temporal replication of ribosomal cistrons in synchronized cell lines

AU - Balazs, I.

AU - Schildkraut, Carl L.

PY - 1976

Y1 - 1976

N2 - The time of synthesis of ribosomal genes was studied in a haploid (Rana pipiens), and a pseudodiploid (Chinese hamster) cell line. R. pipiens cells were synchronized by amethopterin block. Chinese hamster cells were synchronized by isoleucine starvation followed by hydroxyurea treatment. DNA replicated during three or four selected intervals of the S period was separated from the remainder of the DNA by bromodeoxyuridine density labeling. Purified bromodeoxyuridine substituted DNA was annealed with radioactive-labeled 28S ribosomal RNA (rRNA) to determine when, during different intervals of S, the nuclear DNA homologous to rRNA was replicated. In the R. pipiens and Chinese hamster cell lines, the percent of nuclear DNA homologous to 28S rRNA is highest in the DNA replicated during the first half of the S period.

AB - The time of synthesis of ribosomal genes was studied in a haploid (Rana pipiens), and a pseudodiploid (Chinese hamster) cell line. R. pipiens cells were synchronized by amethopterin block. Chinese hamster cells were synchronized by isoleucine starvation followed by hydroxyurea treatment. DNA replicated during three or four selected intervals of the S period was separated from the remainder of the DNA by bromodeoxyuridine density labeling. Purified bromodeoxyuridine substituted DNA was annealed with radioactive-labeled 28S ribosomal RNA (rRNA) to determine when, during different intervals of S, the nuclear DNA homologous to rRNA was replicated. In the R. pipiens and Chinese hamster cell lines, the percent of nuclear DNA homologous to 28S rRNA is highest in the DNA replicated during the first half of the S period.

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