DNA replication in synchronized cultured mammalian cells. III. Relative times of synthesis of mouse satellite and main band DNA

A. M. Tobia, Carl L. Schildkraut, J. J. Maio

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26 Citations (Scopus)

Abstract

Synchronized mouse strain L-cells were pulse-labeled with [14C]thymidine at various stages of the DNA synthetic (S) period. Satellite DNA was then separated from main band DNA by buoyant density gradient centrifugation in Ag+-Cs2SO4 and by CsCl gradient centrifugation of heated and annealed DNA. Centrifugation in analytical alkaline CsCl density gradients showed that the satellite was free of contamination by main band DNA. Changes in the relative specific activities of the main band and pure satellite DNA fractions during the S period confirmed our earlier report that most satellite DNA synthesis occurs after main band DNA has reached its maximum rate of synthesis and did not support the interpretation that the pattern we observed was due to contaminating main band DNA of low G+C content in the satellite DNA fractions. There was no preferential synthesis of satellite DNA in populations of mitotic L-cells.

Original languageEnglish (US)
Pages (from-to)258-262
Number of pages5
JournalBBA Section Nucleic Acids And Protein Synthesis
Volume246
Issue number2
DOIs
StatePublished - Aug 26 1971

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Satellite DNA
DNA Replication
Cultured Cells
DNA
Centrifugation
S Phase
Density Gradient Centrifugation
Base Composition
Thymidine
Population

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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abstract = "Synchronized mouse strain L-cells were pulse-labeled with [14C]thymidine at various stages of the DNA synthetic (S) period. Satellite DNA was then separated from main band DNA by buoyant density gradient centrifugation in Ag+-Cs2SO4 and by CsCl gradient centrifugation of heated and annealed DNA. Centrifugation in analytical alkaline CsCl density gradients showed that the satellite was free of contamination by main band DNA. Changes in the relative specific activities of the main band and pure satellite DNA fractions during the S period confirmed our earlier report that most satellite DNA synthesis occurs after main band DNA has reached its maximum rate of synthesis and did not support the interpretation that the pattern we observed was due to contaminating main band DNA of low G+C content in the satellite DNA fractions. There was no preferential synthesis of satellite DNA in populations of mitotic L-cells.",
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AU - Schildkraut, Carl L.

AU - Maio, J. J.

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