DNA replication in synchronized cultured mammalian cells. II. Replication of ribosomal cistrons in thymidine-synchronized HeLa cells

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Abstract

HeLa cells synchronized by double thymidine treatment were used to determine when DNA homologous to 28 s ribosomal RNA is replicated during S (the synthetic phase of the cell cycle). DNA replicated during selected intervals of the S period was isolated from the remainder of the nuclear DNA by pulse-labeling with bromodeoxyuridine and cesium chloride density-gradient centrifugation. Hybridization of this fractionated DNA with HeLa 28 s RNA indicated that in these cells, DNA homologous to 28 s RNA replicates throughout the S period.

Original languageEnglish (US)
Pages (from-to)153-158
Number of pages6
JournalJournal of Molecular Biology
Volume57
Issue number1
DOIs
StatePublished - Apr 14 1971

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DNA Replication
HeLa Cells
Thymidine
Cultured Cells
S Phase
DNA
Genes
RNA
Artificial Cells
Ribosomal RNA
Density Gradient Centrifugation
Bromodeoxyuridine
Cell Cycle

ASJC Scopus subject areas

  • Virology

Cite this

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abstract = "HeLa cells synchronized by double thymidine treatment were used to determine when DNA homologous to 28 s ribosomal RNA is replicated during S (the synthetic phase of the cell cycle). DNA replicated during selected intervals of the S period was isolated from the remainder of the nuclear DNA by pulse-labeling with bromodeoxyuridine and cesium chloride density-gradient centrifugation. Hybridization of this fractionated DNA with HeLa 28 s RNA indicated that in these cells, DNA homologous to 28 s RNA replicates throughout the S period.",
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