DNA regions flanking the mouse IG 3′ α enhancer are differentially methylated and DNAse I hypersensitive during B cell differentiation

Sandra L. Giannini, Mallika Singh, Charles Félix Calvo, Guifeng Ding, Barbara K. Birshtein

Research output: Contribution to journalArticle

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Abstract

Two B cell-specific enhancer elements are associated with the IgH gene cluster. One enhancer is located within the J-Cμ intron (Eμ), whereas a second enhancer (3′αE) is approximately 12.5 kb 3′ of the Cα membrane exon. In an attempt to understand the function of 3′αE, we have characterized its surrounding structural milieu during various stages of B cell differentiation through analysis of methylation patterns and the identification of DNAse I-hypersensitive sites. We observed a correlation between the chromatin structure of this region and the differentiation state of the cell. Compared to liver and brain, the region 3′ of a was hypermethylated in pre-B and T cell lines and became progressively demethylated as B cell differentiation continued. A DNAse I-hypersensitive site was present in pre-B cell lines about 17 kb 3′ of 3′αE. In fully differentiated myeloma cell lines, a second cluster of DNAse I-hypersensitive sites was present immediately 5′ of 3′αE. Our data indicate that the 3′α enhancer is relatively sequestered during early stages of B cell differentiation and becomes increasingly accessible at later stages.

Original languageEnglish (US)
Pages (from-to)1772-1780
Number of pages9
JournalJournal of Immunology
Volume150
Issue number5
StatePublished - 1993

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Cell Differentiation
B-Lymphocytes
B-Lymphoid Precursor Cells
DNA
Cell Line
Multigene Family
Introns
Methylation
Chromatin
Exons
T-Lymphocytes
Membranes
Liver
Brain

ASJC Scopus subject areas

  • Immunology

Cite this

DNA regions flanking the mouse IG 3′ α enhancer are differentially methylated and DNAse I hypersensitive during B cell differentiation. / Giannini, Sandra L.; Singh, Mallika; Calvo, Charles Félix; Ding, Guifeng; Birshtein, Barbara K.

In: Journal of Immunology, Vol. 150, No. 5, 1993, p. 1772-1780.

Research output: Contribution to journalArticle

Giannini, Sandra L. ; Singh, Mallika ; Calvo, Charles Félix ; Ding, Guifeng ; Birshtein, Barbara K. / DNA regions flanking the mouse IG 3′ α enhancer are differentially methylated and DNAse I hypersensitive during B cell differentiation. In: Journal of Immunology. 1993 ; Vol. 150, No. 5. pp. 1772-1780.
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