DNA methylation profiling using HpaII tiny fragment enrichment by ligation-mediated PCR (HELP)

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The HELP assay is a technique that allows genome-wide analysis of cytosine methylation. Here we describe the assay, its relative strengths and weaknesses, and the transition of the assay from a microarray to massively-parallel sequencing-based foundation.

Original languageEnglish (US)
Pages (from-to)218-222
Number of pages5
JournalMethods
Volume52
Issue number3
DOIs
StatePublished - Nov 2010

Fingerprint

High-Throughput Nucleotide Sequencing
DNA Fingerprinting
Cytosine
DNA Methylation
Methylation
Ligation
Assays
Genome
Polymerase Chain Reaction
Microarrays
Genes

Keywords

  • CpG island
  • Cytosine methylation
  • Epigenetic

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

DNA methylation profiling using HpaII tiny fragment enrichment by ligation-mediated PCR (HELP). / Suzuki, Masako; Greally, John M.

In: Methods, Vol. 52, No. 3, 11.2010, p. 218-222.

Research output: Contribution to journalArticle

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