DNA methylation profiles of donor nuclei cells and tissues of cloned bovine fetuses.

Maksym Kremenskoy, Yuliya Kremenska, Masako Suzuki, Kei Imai, Seiya Takahashi, Kazuyoshi Hashizume, Shintaro Yagi, Kunio Shiota

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Methylation of DNA in CpG islands plays an important role during fetal development and differentiation because CpG islands are preferentially located in upstream regions of mammalian genomic DNA, including the transcription start site of housekeeping genes and are also associated with tissue-specific genes. Somatic nuclear transfer (NT) technology has been used to generate live clones in numerous mammalian species, but only a low percentage of nuclear transferred animals develop to term. Abnormal epigenetic changes in the CpG islands of donor nuclei after nuclear transfer could contribute to a high rate of abortion during early gestation and increase perinatal death. These changes have yet to be explored. Thus, we investigated the genome-wide DNA methylation profiles of CpG islands in nuclei donor cells and NT animals. Using Restriction Landmark Genomic Scanning (RLGS), we showed, for the first time, the epigenetic profile formation of tissues from NT bovine fetuses produced from cumulus cells. From approximately 2600 unmethylated NotI sites visualized on the RLGS profile, at least 35 NotI sites showed different methylation statuses. Moreover, we proved that fetal and placental tissues from artificially inseminated and cloned cattle have tissue-specific differences in the genome-wide methylation profiles of the CpG islands. We also found that possible abnormalities occurred in the fetal brain and placental tissues of cloned animals.

Original languageEnglish (US)
Pages (from-to)259-266
Number of pages8
JournalThe Journal of reproduction and development
Volume52
Issue number2
DOIs
StatePublished - Apr 2006
Externally publishedYes

Fingerprint

CpG Islands
DNA methylation
DNA Methylation
cell nucleus
Cell Nucleus
fetus
Fetus
cattle
genomics
Epigenomics
epigenetics
methylation
Methylation
Genome
Cumulus Cells
Technology Transfer
abortion (animals)
genome
Induced Abortion
Transcription Initiation Site

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Reproductive Medicine

Cite this

DNA methylation profiles of donor nuclei cells and tissues of cloned bovine fetuses. / Kremenskoy, Maksym; Kremenska, Yuliya; Suzuki, Masako; Imai, Kei; Takahashi, Seiya; Hashizume, Kazuyoshi; Yagi, Shintaro; Shiota, Kunio.

In: The Journal of reproduction and development, Vol. 52, No. 2, 04.2006, p. 259-266.

Research output: Contribution to journalArticle

Kremenskoy, M, Kremenska, Y, Suzuki, M, Imai, K, Takahashi, S, Hashizume, K, Yagi, S & Shiota, K 2006, 'DNA methylation profiles of donor nuclei cells and tissues of cloned bovine fetuses.', The Journal of reproduction and development, vol. 52, no. 2, pp. 259-266. https://doi.org/10.1262/jrd.17098
Kremenskoy, Maksym ; Kremenska, Yuliya ; Suzuki, Masako ; Imai, Kei ; Takahashi, Seiya ; Hashizume, Kazuyoshi ; Yagi, Shintaro ; Shiota, Kunio. / DNA methylation profiles of donor nuclei cells and tissues of cloned bovine fetuses. In: The Journal of reproduction and development. 2006 ; Vol. 52, No. 2. pp. 259-266.
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