DNA binding properties of YB-1 and dbpA

Binding to double-stranded, single-stranded, and abasic site containing DNAs

Susan L. Hasegawa, Paul W. Doetsch, Krista K. Hamilton, Amy M. Martin, Sharon A. Okenquist, Jack Lenz, Jeremy M. Boss

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

A number of eukaryotic DNA binding proteins have been isolated by screening phage expression libraries with DNA probes containing the binding site of the DNA-binding protein. This methodology was employed here to isolate clones of the factor that interacts with the W box element of the human major histocompatibility complex HLA-DQB gene. Surprisingly, several cDNA clones of YB-1, a cDNA clone that was previously isolated with a CCAAT element-containing sequence were found. Independently, the screening of phage expression libraries with depurinated DNA resulted in the isolation of YB-1 and dbpA, a previously isolated cDNA that has homology to YB-1. Additional characterization of YB-1 showed that it bound a wide variety of DNA sequences and suggested that the binding of this protein is promiscuous. Furthermore, we show that both YB-1 and dbpA bind to depurinated DNA better than undamaged DNA and that the extent of specificity of binding is influenced by Mg2+. Due to the lack of sequence specificity and high degree of binding to depurinated DNA, we suggest that these proteins might be involved in chromosome functions such as maintenance of chromatin structure or DNA repair that do not require sequence-specific binding.

Original languageEnglish (US)
Pages (from-to)4915-4920
Number of pages6
JournalNucleic Acids Research
Volume19
Issue number18
StatePublished - Sep 25 1991

Fingerprint

DNA
CDNA
Clone
Complementary DNA
Clone Cells
DNA-Binding Proteins
DNA-binding Protein
Bacteriophages
Specificity
Screening
DNA Probes
Protein
Major Histocompatibility Complex
DNA Repair
Chromatin
DNA sequences
Chromosomes
Carrier Proteins
DNA Sequence
Binding Sites

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Cite this

Hasegawa, S. L., Doetsch, P. W., Hamilton, K. K., Martin, A. M., Okenquist, S. A., Lenz, J., & Boss, J. M. (1991). DNA binding properties of YB-1 and dbpA: Binding to double-stranded, single-stranded, and abasic site containing DNAs. Nucleic Acids Research, 19(18), 4915-4920.

DNA binding properties of YB-1 and dbpA : Binding to double-stranded, single-stranded, and abasic site containing DNAs. / Hasegawa, Susan L.; Doetsch, Paul W.; Hamilton, Krista K.; Martin, Amy M.; Okenquist, Sharon A.; Lenz, Jack; Boss, Jeremy M.

In: Nucleic Acids Research, Vol. 19, No. 18, 25.09.1991, p. 4915-4920.

Research output: Contribution to journalArticle

Hasegawa, SL, Doetsch, PW, Hamilton, KK, Martin, AM, Okenquist, SA, Lenz, J & Boss, JM 1991, 'DNA binding properties of YB-1 and dbpA: Binding to double-stranded, single-stranded, and abasic site containing DNAs', Nucleic Acids Research, vol. 19, no. 18, pp. 4915-4920.
Hasegawa SL, Doetsch PW, Hamilton KK, Martin AM, Okenquist SA, Lenz J et al. DNA binding properties of YB-1 and dbpA: Binding to double-stranded, single-stranded, and abasic site containing DNAs. Nucleic Acids Research. 1991 Sep 25;19(18):4915-4920.
Hasegawa, Susan L. ; Doetsch, Paul W. ; Hamilton, Krista K. ; Martin, Amy M. ; Okenquist, Sharon A. ; Lenz, Jack ; Boss, Jeremy M. / DNA binding properties of YB-1 and dbpA : Binding to double-stranded, single-stranded, and abasic site containing DNAs. In: Nucleic Acids Research. 1991 ; Vol. 19, No. 18. pp. 4915-4920.
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