Diverse enterotoxin gene profiles among clonal complexes of Staphylococcus aureus isolates from the Bronx, New York

Avanish K. Varshney, José R. Mediavilla, Natalie Robiou, Alice Guh, Xiabo Wang, Philip Gialanella, Michael H. Levi, Barry N. Kreiswirth, Bettina C. Fries

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Staphylococcal enterotoxins (SE) can cause toxin-mediated disease, and those that function as superantigens are implicated in the pathogenesis of allergic diseases. The prevalence of 19 enterotoxin genes was determined by PCR in clinical S. aureus strains derived from wounds (108) and blood (99). We performed spa typing and multilocus sequence typing (MLST) to determine clonal origin, and for selected strains staphylococcal enterotoxin B (SEB) production was measured by enzyme-linked immunosorbent assay. Strains carried a median of five SE genes. For most SE genes, the prevalence rates among methicillinresistant and methicillin-sensitive S. aureus isolates, as well as wound- and blood-derived isolates, did not differ. At least one SE gene was detected in all except two S. aureus isolates (>99%). Complete egc clusters were found in only 11% of S. aureus isolates, whereas the combination of sed, sej, and ser was detected in 24% of clinical strains. S. aureus strains exhibited distinct combinations of SE genes, even if their pulsed-field gel electrophoresis and MLST patterns demonstrated clonality. USA300 strains also showed considerable variability in SE content, although they contained a lower number of SE genes (mean, 3). By contrast, SE content was unchanged in five pairs of serial isolates. SEB production by individual strains varied up to 200-fold, and even up to 15-fold in a pair of serial isolates. In conclusion, our results illustrate the genetic diversity of S. aureus strains with respect to enterotoxin genes and suggest that horizontal transfer of mobile genetic elements encoding virulence genes occurs frequently.

Original languageEnglish (US)
Pages (from-to)6839-6849
Number of pages11
JournalApplied and Environmental Microbiology
Volume75
Issue number21
DOIs
StatePublished - Nov 2009

Fingerprint

Enterotoxins
enterotoxins
Staphylococcus aureus
gene
Genes
genes
Multilocus Sequence Typing
blood
fold
animal injuries
Interspersed Repetitive Sequences
Horizontal Gene Transfer
virulence
Superantigens
Methicillin
superantigens
toxin
Pulsed Field Gel Electrophoresis
Wounds and Injuries
electrokinesis

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Food Science
  • Biotechnology
  • Ecology

Cite this

Diverse enterotoxin gene profiles among clonal complexes of Staphylococcus aureus isolates from the Bronx, New York. / Varshney, Avanish K.; Mediavilla, José R.; Robiou, Natalie; Guh, Alice; Wang, Xiabo; Gialanella, Philip; Levi, Michael H.; Kreiswirth, Barry N.; Fries, Bettina C.

In: Applied and Environmental Microbiology, Vol. 75, No. 21, 11.2009, p. 6839-6849.

Research output: Contribution to journalArticle

Varshney, AK, Mediavilla, JR, Robiou, N, Guh, A, Wang, X, Gialanella, P, Levi, MH, Kreiswirth, BN & Fries, BC 2009, 'Diverse enterotoxin gene profiles among clonal complexes of Staphylococcus aureus isolates from the Bronx, New York', Applied and Environmental Microbiology, vol. 75, no. 21, pp. 6839-6849. https://doi.org/10.1128/AEM.00272-09
Varshney, Avanish K. ; Mediavilla, José R. ; Robiou, Natalie ; Guh, Alice ; Wang, Xiabo ; Gialanella, Philip ; Levi, Michael H. ; Kreiswirth, Barry N. ; Fries, Bettina C. / Diverse enterotoxin gene profiles among clonal complexes of Staphylococcus aureus isolates from the Bronx, New York. In: Applied and Environmental Microbiology. 2009 ; Vol. 75, No. 21. pp. 6839-6849.
@article{650bd0023f8648eb8e1a5d866d718d73,
title = "Diverse enterotoxin gene profiles among clonal complexes of Staphylococcus aureus isolates from the Bronx, New York",
abstract = "Staphylococcal enterotoxins (SE) can cause toxin-mediated disease, and those that function as superantigens are implicated in the pathogenesis of allergic diseases. The prevalence of 19 enterotoxin genes was determined by PCR in clinical S. aureus strains derived from wounds (108) and blood (99). We performed spa typing and multilocus sequence typing (MLST) to determine clonal origin, and for selected strains staphylococcal enterotoxin B (SEB) production was measured by enzyme-linked immunosorbent assay. Strains carried a median of five SE genes. For most SE genes, the prevalence rates among methicillinresistant and methicillin-sensitive S. aureus isolates, as well as wound- and blood-derived isolates, did not differ. At least one SE gene was detected in all except two S. aureus isolates (>99{\%}). Complete egc clusters were found in only 11{\%} of S. aureus isolates, whereas the combination of sed, sej, and ser was detected in 24{\%} of clinical strains. S. aureus strains exhibited distinct combinations of SE genes, even if their pulsed-field gel electrophoresis and MLST patterns demonstrated clonality. USA300 strains also showed considerable variability in SE content, although they contained a lower number of SE genes (mean, 3). By contrast, SE content was unchanged in five pairs of serial isolates. SEB production by individual strains varied up to 200-fold, and even up to 15-fold in a pair of serial isolates. In conclusion, our results illustrate the genetic diversity of S. aureus strains with respect to enterotoxin genes and suggest that horizontal transfer of mobile genetic elements encoding virulence genes occurs frequently.",
author = "Varshney, {Avanish K.} and Mediavilla, {Jos{\'e} R.} and Natalie Robiou and Alice Guh and Xiabo Wang and Philip Gialanella and Levi, {Michael H.} and Kreiswirth, {Barry N.} and Fries, {Bettina C.}",
year = "2009",
month = "11",
doi = "10.1128/AEM.00272-09",
language = "English (US)",
volume = "75",
pages = "6839--6849",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "21",

}

TY - JOUR

T1 - Diverse enterotoxin gene profiles among clonal complexes of Staphylococcus aureus isolates from the Bronx, New York

AU - Varshney, Avanish K.

AU - Mediavilla, José R.

AU - Robiou, Natalie

AU - Guh, Alice

AU - Wang, Xiabo

AU - Gialanella, Philip

AU - Levi, Michael H.

AU - Kreiswirth, Barry N.

AU - Fries, Bettina C.

PY - 2009/11

Y1 - 2009/11

N2 - Staphylococcal enterotoxins (SE) can cause toxin-mediated disease, and those that function as superantigens are implicated in the pathogenesis of allergic diseases. The prevalence of 19 enterotoxin genes was determined by PCR in clinical S. aureus strains derived from wounds (108) and blood (99). We performed spa typing and multilocus sequence typing (MLST) to determine clonal origin, and for selected strains staphylococcal enterotoxin B (SEB) production was measured by enzyme-linked immunosorbent assay. Strains carried a median of five SE genes. For most SE genes, the prevalence rates among methicillinresistant and methicillin-sensitive S. aureus isolates, as well as wound- and blood-derived isolates, did not differ. At least one SE gene was detected in all except two S. aureus isolates (>99%). Complete egc clusters were found in only 11% of S. aureus isolates, whereas the combination of sed, sej, and ser was detected in 24% of clinical strains. S. aureus strains exhibited distinct combinations of SE genes, even if their pulsed-field gel electrophoresis and MLST patterns demonstrated clonality. USA300 strains also showed considerable variability in SE content, although they contained a lower number of SE genes (mean, 3). By contrast, SE content was unchanged in five pairs of serial isolates. SEB production by individual strains varied up to 200-fold, and even up to 15-fold in a pair of serial isolates. In conclusion, our results illustrate the genetic diversity of S. aureus strains with respect to enterotoxin genes and suggest that horizontal transfer of mobile genetic elements encoding virulence genes occurs frequently.

AB - Staphylococcal enterotoxins (SE) can cause toxin-mediated disease, and those that function as superantigens are implicated in the pathogenesis of allergic diseases. The prevalence of 19 enterotoxin genes was determined by PCR in clinical S. aureus strains derived from wounds (108) and blood (99). We performed spa typing and multilocus sequence typing (MLST) to determine clonal origin, and for selected strains staphylococcal enterotoxin B (SEB) production was measured by enzyme-linked immunosorbent assay. Strains carried a median of five SE genes. For most SE genes, the prevalence rates among methicillinresistant and methicillin-sensitive S. aureus isolates, as well as wound- and blood-derived isolates, did not differ. At least one SE gene was detected in all except two S. aureus isolates (>99%). Complete egc clusters were found in only 11% of S. aureus isolates, whereas the combination of sed, sej, and ser was detected in 24% of clinical strains. S. aureus strains exhibited distinct combinations of SE genes, even if their pulsed-field gel electrophoresis and MLST patterns demonstrated clonality. USA300 strains also showed considerable variability in SE content, although they contained a lower number of SE genes (mean, 3). By contrast, SE content was unchanged in five pairs of serial isolates. SEB production by individual strains varied up to 200-fold, and even up to 15-fold in a pair of serial isolates. In conclusion, our results illustrate the genetic diversity of S. aureus strains with respect to enterotoxin genes and suggest that horizontal transfer of mobile genetic elements encoding virulence genes occurs frequently.

UR - http://www.scopus.com/inward/record.url?scp=70350518256&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70350518256&partnerID=8YFLogxK

U2 - 10.1128/AEM.00272-09

DO - 10.1128/AEM.00272-09

M3 - Article

C2 - 19749060

AN - SCOPUS:70350518256

VL - 75

SP - 6839

EP - 6849

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 21

ER -