Diverse CD1d-restricted reactivity patterns of human T cells bearing 'invariant' AV24BV11 TCR

Christelle Couedel, Marie Alix Peyrat, Laurent Brossay, Yasuhiko Koezuka, Steven A. Porcelli, François Davodeau, Marc Bonneville

Research output: Contribution to journalArticle

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Abstract

Human and murine natural T (NT) cells, also referred to as NK1.1+ or NK T cells, express TCR with homologous V regions (hAV24/BV11 and mAV14/BV8, respectively) and conserved 'invariant' TCR AVAJ junctional sequences, suggesting recognition of closely related antigens. Murine NT cells recognize CD1-expressing cells and are activated in a CD1-restricted fashion by several synthetic α-glycosylceramides, such as α-GalCer. Here we studied the reactivity of human T cells against CD1d+ cells pulsed or not with α-GalCer and other related ceramides. CD1d-restricted recognition of α-GalCer was a general and specific feature of T cell clones expressing both BV11 and canonical AV24AJ18 TCR chains. Besides, human and murine NT cells showed the same reactivity patterns against a set of related glycosylceramides, suggesting a highly conserved mode of recognition of these antigens in humans and rodents. We also identified several AV24BV11 T cell clones self reactive against CD1+ cells of both hemopoietic and nonhemopoietic origin, suggesting the existence of distinct NT cell subsets differing by their ability to recognize self CD1d molecules.

Original languageEnglish (US)
Pages (from-to)4391-4397
Number of pages7
JournalEuropean Journal of Immunology
Volume28
Issue number12
StatePublished - Dec 1998
Externally publishedYes

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T-Lymphocytes
CD1d Antigen
Clone Cells
Antigens
Ceramides
T-Lymphocyte Subsets
Natural Killer Cells
Rodentia

Keywords

  • CD1
  • Glycosylceramide
  • NKT cell
  • TCR

ASJC Scopus subject areas

  • Immunology

Cite this

Couedel, C., Peyrat, M. A., Brossay, L., Koezuka, Y., Porcelli, S. A., Davodeau, F., & Bonneville, M. (1998). Diverse CD1d-restricted reactivity patterns of human T cells bearing 'invariant' AV24BV11 TCR. European Journal of Immunology, 28(12), 4391-4397.

Diverse CD1d-restricted reactivity patterns of human T cells bearing 'invariant' AV24BV11 TCR. / Couedel, Christelle; Peyrat, Marie Alix; Brossay, Laurent; Koezuka, Yasuhiko; Porcelli, Steven A.; Davodeau, François; Bonneville, Marc.

In: European Journal of Immunology, Vol. 28, No. 12, 12.1998, p. 4391-4397.

Research output: Contribution to journalArticle

Couedel, C, Peyrat, MA, Brossay, L, Koezuka, Y, Porcelli, SA, Davodeau, F & Bonneville, M 1998, 'Diverse CD1d-restricted reactivity patterns of human T cells bearing 'invariant' AV24BV11 TCR', European Journal of Immunology, vol. 28, no. 12, pp. 4391-4397.
Couedel C, Peyrat MA, Brossay L, Koezuka Y, Porcelli SA, Davodeau F et al. Diverse CD1d-restricted reactivity patterns of human T cells bearing 'invariant' AV24BV11 TCR. European Journal of Immunology. 1998 Dec;28(12):4391-4397.
Couedel, Christelle ; Peyrat, Marie Alix ; Brossay, Laurent ; Koezuka, Yasuhiko ; Porcelli, Steven A. ; Davodeau, François ; Bonneville, Marc. / Diverse CD1d-restricted reactivity patterns of human T cells bearing 'invariant' AV24BV11 TCR. In: European Journal of Immunology. 1998 ; Vol. 28, No. 12. pp. 4391-4397.
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