Divergent regulatton of the murine CC chemokine C10 by Th1 and Th2 cytokines

Amos Orlofsky, Yaqing Wu, Michael B. Prystowsky

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Chemokines are typically found as products of acute stimulation of host defence cells. In contrast, the mouse CC chemokine C10 was previously shown to be a delayed, stably induced product of macrophages treated with interleukin 3 (IL-3), IL-4 or GM-CSF. We investigated the possibility that C10 is differentially regulated by cytokines associated with Th1 and Th2 cells. Northern blot analysis of bone marrow-derived macrophages showed that, in addition to IL-4, the Th2-specific cytokines IL-10 and IL-13 upregulated C10 over a 48-h period in a dose-dependent manner. In contrast, MIP-1α and MCP-1/JE were induced by IL-3 or GM-CSF at 48 h and this induction was inhibited by IL-4. Interferon γ, a Th1-specific product, abolished the induction of C10 mRNA and protein by either IL-3 or granulocyte-macrophage colony-stimulating factor (GM-CSF) in either bone marrow-derived or peritoneal macrophages. The inhibition of C10 production by interferon γ was not NO dependent. Finally the GM-CSF-mediated induction of C10 in peritoneal macrophages was eliminated when these cells presented antigen to established T cells of Th1 phenotype. The findings are consistent with a potential role for C10 in the modulation of immune reactions of Th2 type. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)220-228
Number of pages9
JournalCytokine
Volume12
Issue number3
DOIs
StatePublished - Mar 2000

Keywords

  • Chemokines
  • Interferon γ
  • Interleukin 4
  • Macrophages
  • Th

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

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