Divergent regulatton of the murine CC chemokine C10 by Th1 and Th2 cytokines

Amos Orlofsky, Yaqing Wu, Michael B. Prystowsky

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Chemokines are typically found as products of acute stimulation of host defence cells. In contrast, the mouse CC chemokine C10 was previously shown to be a delayed, stably induced product of macrophages treated with interleukin 3 (IL-3), IL-4 or GM-CSF. We investigated the possibility that C10 is differentially regulated by cytokines associated with Th1 and Th2 cells. Northern blot analysis of bone marrow-derived macrophages showed that, in addition to IL-4, the Th2-specific cytokines IL-10 and IL-13 upregulated C10 over a 48-h period in a dose-dependent manner. In contrast, MIP-1α and MCP-1/JE were induced by IL-3 or GM-CSF at 48 h and this induction was inhibited by IL-4. Interferon γ, a Th1-specific product, abolished the induction of C10 mRNA and protein by either IL-3 or granulocyte-macrophage colony-stimulating factor (GM-CSF) in either bone marrow-derived or peritoneal macrophages. The inhibition of C10 production by interferon γ was not NO dependent. Finally the GM-CSF-mediated induction of C10 in peritoneal macrophages was eliminated when these cells presented antigen to established T cells of Th1 phenotype. The findings are consistent with a potential role for C10 in the modulation of immune reactions of Th2 type. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)220-228
Number of pages9
JournalCytokine
Volume12
Issue number3
DOIs
StatePublished - Mar 2000

Keywords

  • Chemokines
  • Interferon γ
  • Interleukin 4
  • Macrophages
  • Th

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

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