Divergence of transcription factor binding sites across related yeast species

Anthony R. Borneman, Tara A. Gianoulis, Zhengdong Zhang, Haiyuan Yu, Joel Rozowsky, Michael R. Seringhaus, Yong Wang Lu, Mark Gerstein, Michael Snyder

Research output: Contribution to journalArticle

262 Citations (Scopus)

Abstract

Characterization of interspecies differences in gene regulation is crucial for understanding the molecular basis of both phenotypic diversity and evolution. By means of chromatin immunoprecipitation and DNA microarray analysis, the divergence in the binding sites of the pseudohyphal regulators Ste12 and Tec1 was determined in the yeasts Saccharomyces cerevisiae, S. mikatae, and S. bayanus under pseudohyphal conditions. We have shown that most of these sites have diverged across these species, far exceeding the interspecies variation in orthologous genes. A group of Stel2 targets was shown to be bound only in S. mikatae and S. bayanus under pseudohyphal conditions. Many of these genes are targets of Ste12 during mating in S. cerevisiae, indicating that specialization between the two pathways has occurred in this species. Transcription factor binding sites have therefore diverged substantially faster than ortholog content. Thus, gene regulation resulting from transcription factor binding is likely to be a major cause of divergence between related species.

Original languageEnglish (US)
Pages (from-to)815-819
Number of pages5
JournalScience
Volume317
Issue number5839
DOIs
StatePublished - Aug 10 2007
Externally publishedYes

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Transcription Factors
Yeasts
Binding Sites
Genes
Saccharomyces cerevisiae
Chromatin Immunoprecipitation
Microarray Analysis
Oligonucleotide Array Sequence Analysis

ASJC Scopus subject areas

  • General

Cite this

Borneman, A. R., Gianoulis, T. A., Zhang, Z., Yu, H., Rozowsky, J., Seringhaus, M. R., ... Snyder, M. (2007). Divergence of transcription factor binding sites across related yeast species. Science, 317(5839), 815-819. https://doi.org/10.1126/science.1140748

Divergence of transcription factor binding sites across related yeast species. / Borneman, Anthony R.; Gianoulis, Tara A.; Zhang, Zhengdong; Yu, Haiyuan; Rozowsky, Joel; Seringhaus, Michael R.; Lu, Yong Wang; Gerstein, Mark; Snyder, Michael.

In: Science, Vol. 317, No. 5839, 10.08.2007, p. 815-819.

Research output: Contribution to journalArticle

Borneman, AR, Gianoulis, TA, Zhang, Z, Yu, H, Rozowsky, J, Seringhaus, MR, Lu, YW, Gerstein, M & Snyder, M 2007, 'Divergence of transcription factor binding sites across related yeast species', Science, vol. 317, no. 5839, pp. 815-819. https://doi.org/10.1126/science.1140748
Borneman AR, Gianoulis TA, Zhang Z, Yu H, Rozowsky J, Seringhaus MR et al. Divergence of transcription factor binding sites across related yeast species. Science. 2007 Aug 10;317(5839):815-819. https://doi.org/10.1126/science.1140748
Borneman, Anthony R. ; Gianoulis, Tara A. ; Zhang, Zhengdong ; Yu, Haiyuan ; Rozowsky, Joel ; Seringhaus, Michael R. ; Lu, Yong Wang ; Gerstein, Mark ; Snyder, Michael. / Divergence of transcription factor binding sites across related yeast species. In: Science. 2007 ; Vol. 317, No. 5839. pp. 815-819.
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