Disulfide bonds are required for Serratia marcescens nuclease activity

Timothy K. Ball; Yousin Suh; Michael J. Benedik

doi:10.1093/nar/20.19.4971

Disulfide bonds are required for Serratia marcescens nuclease activity

Timothy K. Ball, Yousin Suh, Michael J. Benedik

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

The role of the two disulfide bonds found in the Serratia marcescens nuclease were tested by site directed mutagenesis and were found essential for nuclease activity, although slight residual activity remained. The requirement for disulfide bond formation may play a role in preventing the lethal action of nuclease while in the bacterial cytoplasm.

Original language	English (US)
Pages (from-to)	4971-4974
Number of pages	4
Journal	Nucleic acids research
Volume	20
Issue number	19
DOIs	https://doi.org/10.1093/nar/20.19.4971
State	Published - Oct 11 1992
Externally published	Yes

ASJC Scopus subject areas

Genetics

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title = "Disulfide bonds are required for Serratia marcescens nuclease activity",

abstract = "The role of the two disulfide bonds found in the Serratia marcescens nuclease were tested by site directed mutagenesis and were found essential for nuclease activity, although slight residual activity remained. The requirement for disulfide bond formation may play a role in preventing the lethal action of nuclease while in the bacterial cytoplasm.",

author = "Ball, {Timothy K.} and Yousin Suh and Benedik, {Michael J.}",

note = "Funding Information: This work was supported by grant GM36891 from the National Institutes of Health and awards 3652-117 and 3652-233 through the Texas Advanced Research Program. Some equipment used in this work were purchased through equipment grant DIR-9109294 from the National Science Foundation.",

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doi = "10.1093/nar/20.19.4971",

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AU - Suh, Yousin

AU - Benedik, Michael J.

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PY - 1992/10/11

Y1 - 1992/10/11

N2 - The role of the two disulfide bonds found in the Serratia marcescens nuclease were tested by site directed mutagenesis and were found essential for nuclease activity, although slight residual activity remained. The requirement for disulfide bond formation may play a role in preventing the lethal action of nuclease while in the bacterial cytoplasm.

AB - The role of the two disulfide bonds found in the Serratia marcescens nuclease were tested by site directed mutagenesis and were found essential for nuclease activity, although slight residual activity remained. The requirement for disulfide bond formation may play a role in preventing the lethal action of nuclease while in the bacterial cytoplasm.

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Disulfide bonds are required for Serratia marcescens nuclease activity

Abstract

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