Distribution of tunichrome and vanadium in sea squirt blood cells sorted by flow cytometry

E. M. Oltz, Simeon Pollack, T. Delohery, M. J. Smith, M. Ojika, S. Lee, K. Kustin, K. Nakanishi

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Specialized blood cells of many tunicates accumulate high concentrations of vanadium and phenolic peptide pigments called tunichromes (TC). In order to determine whether V and TC reside in the same cells, Ascidia nigra and Ascidia ceratodes blood cell subpopulations were isolated by fluorescence-activated cell sorting (flow cytometry) and chemically analyzed. V was found in the spherical, gree/grey signet ring cells, and to a lesser degree in the mulberry-shaped, yellow/ green morula cells (MRs), whereas free TC was detected mainly in MRs.

Original languageEnglish (US)
Pages (from-to)186-190
Number of pages5
JournalExperientia
Volume45
Issue number2
DOIs
StatePublished - Feb 1989

Fingerprint

Urochordata
Vanadium
Flow cytometry
Oceans and Seas
Blood Cells
Flow Cytometry
Blood
Cells
Morus
Morula
Sorting
Pigments
Fluorescence
Peptides
tunichrome

Keywords

  • Fluorescence activated cell sorting (FACS)
  • morula cells
  • signet ring cells
  • tunicates
  • vanadium

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Oltz, E. M., Pollack, S., Delohery, T., Smith, M. J., Ojika, M., Lee, S., ... Nakanishi, K. (1989). Distribution of tunichrome and vanadium in sea squirt blood cells sorted by flow cytometry. Experientia, 45(2), 186-190. https://doi.org/10.1007/BF01954871

Distribution of tunichrome and vanadium in sea squirt blood cells sorted by flow cytometry. / Oltz, E. M.; Pollack, Simeon; Delohery, T.; Smith, M. J.; Ojika, M.; Lee, S.; Kustin, K.; Nakanishi, K.

In: Experientia, Vol. 45, No. 2, 02.1989, p. 186-190.

Research output: Contribution to journalArticle

Oltz, EM, Pollack, S, Delohery, T, Smith, MJ, Ojika, M, Lee, S, Kustin, K & Nakanishi, K 1989, 'Distribution of tunichrome and vanadium in sea squirt blood cells sorted by flow cytometry', Experientia, vol. 45, no. 2, pp. 186-190. https://doi.org/10.1007/BF01954871
Oltz EM, Pollack S, Delohery T, Smith MJ, Ojika M, Lee S et al. Distribution of tunichrome and vanadium in sea squirt blood cells sorted by flow cytometry. Experientia. 1989 Feb;45(2):186-190. https://doi.org/10.1007/BF01954871
Oltz, E. M. ; Pollack, Simeon ; Delohery, T. ; Smith, M. J. ; Ojika, M. ; Lee, S. ; Kustin, K. ; Nakanishi, K. / Distribution of tunichrome and vanadium in sea squirt blood cells sorted by flow cytometry. In: Experientia. 1989 ; Vol. 45, No. 2. pp. 186-190.
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