Distribution of tunichrome and vanadium in sea squirt blood cells sorted by flow cytometry

E. M. Oltz, S. Pollack, T. Delohery, M. J. Smith, M. Ojika, S. Lee, K. Kustin, K. Nakanishi

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

Specialized blood cells of many tunicates accumulate high concentrations of vanadium and phenolic peptide pigments called tunichromes (TC). In order to determine whether V and TC reside in the same cells, Ascidia nigra and Ascidia ceratodes blood cell subpopulations were isolated by fluorescence-activated cell sorting (flow cytometry) and chemically analyzed. V was found in the spherical, gree/grey signet ring cells, and to a lesser degree in the mulberry-shaped, yellow/ green morula cells (MRs), whereas free TC was detected mainly in MRs.

Original languageEnglish (US)
Pages (from-to)186-190
Number of pages5
JournalExperientia
Volume45
Issue number2
DOIs
StatePublished - Feb 1 1989

Keywords

  • Fluorescence activated cell sorting (FACS)
  • morula cells
  • signet ring cells
  • tunicates
  • vanadium

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Oltz, E. M., Pollack, S., Delohery, T., Smith, M. J., Ojika, M., Lee, S., Kustin, K., & Nakanishi, K. (1989). Distribution of tunichrome and vanadium in sea squirt blood cells sorted by flow cytometry. Experientia, 45(2), 186-190. https://doi.org/10.1007/BF01954871