Distribution of protein kinase Mζ and the complete protein kinase C isoform family in rat brain

M. A. Howard, I. O. Volkov, R. Mirsky, P. C. Garell, M. D. Noh, M. Granner, H. Damasio, Mitchell Steinschneider, R. A. Reale, J. E. Hind, J. F. Brugge

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Protein kinase C (pkc) is a multigene family of at least ten isoforms, nine of which are expressed in brain (α, βi, βii, γ, δ, ̇, η, ζ, ι/λ). Our previous studies have shown that many of these Pkcs participate in synaptic plasticity in the Ca1 region of the hippocampus. Multiple isoforms are transiently activated in the induction phase of long-term potentiation (ltp). In contrast, a single species, ζ, is persistently activated during the maintenance phase of Ltp through the formation of an independent, constitutively active catalytic domain, protein kinase Mζ (pkmζ). In this study, we used immunoblot and immunocytochemical techniques with isoform-specific antisera to examine the distribution of the complete family of Pkc isozymes and Pkmζ in rat brain. Each form of Pkc showed a widespread distribution in the brain with a distinct regional pattern of high and low levels of expression. Pkmζ, the predominant form of Pkm in brain, had high levels in hippocampus, frontal and occipital cortex, striatum, and hypothalamus. In the hippocampus, each isoform was expressed in a characteristic pattern, with ζ prominent in the Ca1 stratum radiatum. These results suggest that the compartmentalization of Pkc isoforms in neurons may contribute to their function, with the location of Pkmζ prominent in areas notable for long-term synaptic plasticity. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)243-258
Number of pages16
JournalJournal of Comparative Neurology
Volume426
Issue number2
DOIs
StatePublished - Oct 16 2000

Fingerprint

Protein Kinase C
Protein Isoforms
Brain
Hippocampus
Neuronal Plasticity
Occipital Lobe
Long-Term Potentiation
Frontal Lobe
Multigene Family
Isoenzymes
Hypothalamus
Immune Sera
Catalytic Domain
Maintenance
Neurons

Keywords

  • Hippocampus
  • Long-term potentiation (LTP)
  • Protein kinase C zeta
  • Protein kinase M zeta
  • Purkinje cell
  • Pyramidal cell

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Distribution of protein kinase Mζ and the complete protein kinase C isoform family in rat brain. / Howard, M. A.; Volkov, I. O.; Mirsky, R.; Garell, P. C.; Noh, M. D.; Granner, M.; Damasio, H.; Steinschneider, Mitchell; Reale, R. A.; Hind, J. E.; Brugge, J. F.

In: Journal of Comparative Neurology, Vol. 426, No. 2, 16.10.2000, p. 243-258.

Research output: Contribution to journalArticle

Howard, MA, Volkov, IO, Mirsky, R, Garell, PC, Noh, MD, Granner, M, Damasio, H, Steinschneider, M, Reale, RA, Hind, JE & Brugge, JF 2000, 'Distribution of protein kinase Mζ and the complete protein kinase C isoform family in rat brain', Journal of Comparative Neurology, vol. 426, no. 2, pp. 243-258. https://doi.org/10.1002/(SICI)1096-9861(20000103)416:1<79::AID-CNE6>3.0.CO;2-2
Howard, M. A. ; Volkov, I. O. ; Mirsky, R. ; Garell, P. C. ; Noh, M. D. ; Granner, M. ; Damasio, H. ; Steinschneider, Mitchell ; Reale, R. A. ; Hind, J. E. ; Brugge, J. F. / Distribution of protein kinase Mζ and the complete protein kinase C isoform family in rat brain. In: Journal of Comparative Neurology. 2000 ; Vol. 426, No. 2. pp. 243-258.
@article{3d466e7a0cb14c60ad651ede806ab0d6,
title = "Distribution of protein kinase Mζ and the complete protein kinase C isoform family in rat brain",
abstract = "Protein kinase C (pkc) is a multigene family of at least ten isoforms, nine of which are expressed in brain (α, βi, βii, γ, δ, ̇, η, ζ, ι/λ). Our previous studies have shown that many of these Pkcs participate in synaptic plasticity in the Ca1 region of the hippocampus. Multiple isoforms are transiently activated in the induction phase of long-term potentiation (ltp). In contrast, a single species, ζ, is persistently activated during the maintenance phase of Ltp through the formation of an independent, constitutively active catalytic domain, protein kinase Mζ (pkmζ). In this study, we used immunoblot and immunocytochemical techniques with isoform-specific antisera to examine the distribution of the complete family of Pkc isozymes and Pkmζ in rat brain. Each form of Pkc showed a widespread distribution in the brain with a distinct regional pattern of high and low levels of expression. Pkmζ, the predominant form of Pkm in brain, had high levels in hippocampus, frontal and occipital cortex, striatum, and hypothalamus. In the hippocampus, each isoform was expressed in a characteristic pattern, with ζ prominent in the Ca1 stratum radiatum. These results suggest that the compartmentalization of Pkc isoforms in neurons may contribute to their function, with the location of Pkmζ prominent in areas notable for long-term synaptic plasticity. (C) 2000 Wiley-Liss, Inc.",
keywords = "Hippocampus, Long-term potentiation (LTP), Protein kinase C zeta, Protein kinase M zeta, Purkinje cell, Pyramidal cell",
author = "Howard, {M. A.} and Volkov, {I. O.} and R. Mirsky and Garell, {P. C.} and Noh, {M. D.} and M. Granner and H. Damasio and Mitchell Steinschneider and Reale, {R. A.} and Hind, {J. E.} and Brugge, {J. F.}",
year = "2000",
month = "10",
day = "16",
doi = "10.1002/(SICI)1096-9861(20000103)416:1<79::AID-CNE6>3.0.CO;2-2",
language = "English (US)",
volume = "426",
pages = "243--258",
journal = "Journal of Comparative Neurology",
issn = "0021-9967",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - Distribution of protein kinase Mζ and the complete protein kinase C isoform family in rat brain

AU - Howard, M. A.

AU - Volkov, I. O.

AU - Mirsky, R.

AU - Garell, P. C.

AU - Noh, M. D.

AU - Granner, M.

AU - Damasio, H.

AU - Steinschneider, Mitchell

AU - Reale, R. A.

AU - Hind, J. E.

AU - Brugge, J. F.

PY - 2000/10/16

Y1 - 2000/10/16

N2 - Protein kinase C (pkc) is a multigene family of at least ten isoforms, nine of which are expressed in brain (α, βi, βii, γ, δ, ̇, η, ζ, ι/λ). Our previous studies have shown that many of these Pkcs participate in synaptic plasticity in the Ca1 region of the hippocampus. Multiple isoforms are transiently activated in the induction phase of long-term potentiation (ltp). In contrast, a single species, ζ, is persistently activated during the maintenance phase of Ltp through the formation of an independent, constitutively active catalytic domain, protein kinase Mζ (pkmζ). In this study, we used immunoblot and immunocytochemical techniques with isoform-specific antisera to examine the distribution of the complete family of Pkc isozymes and Pkmζ in rat brain. Each form of Pkc showed a widespread distribution in the brain with a distinct regional pattern of high and low levels of expression. Pkmζ, the predominant form of Pkm in brain, had high levels in hippocampus, frontal and occipital cortex, striatum, and hypothalamus. In the hippocampus, each isoform was expressed in a characteristic pattern, with ζ prominent in the Ca1 stratum radiatum. These results suggest that the compartmentalization of Pkc isoforms in neurons may contribute to their function, with the location of Pkmζ prominent in areas notable for long-term synaptic plasticity. (C) 2000 Wiley-Liss, Inc.

AB - Protein kinase C (pkc) is a multigene family of at least ten isoforms, nine of which are expressed in brain (α, βi, βii, γ, δ, ̇, η, ζ, ι/λ). Our previous studies have shown that many of these Pkcs participate in synaptic plasticity in the Ca1 region of the hippocampus. Multiple isoforms are transiently activated in the induction phase of long-term potentiation (ltp). In contrast, a single species, ζ, is persistently activated during the maintenance phase of Ltp through the formation of an independent, constitutively active catalytic domain, protein kinase Mζ (pkmζ). In this study, we used immunoblot and immunocytochemical techniques with isoform-specific antisera to examine the distribution of the complete family of Pkc isozymes and Pkmζ in rat brain. Each form of Pkc showed a widespread distribution in the brain with a distinct regional pattern of high and low levels of expression. Pkmζ, the predominant form of Pkm in brain, had high levels in hippocampus, frontal and occipital cortex, striatum, and hypothalamus. In the hippocampus, each isoform was expressed in a characteristic pattern, with ζ prominent in the Ca1 stratum radiatum. These results suggest that the compartmentalization of Pkc isoforms in neurons may contribute to their function, with the location of Pkmζ prominent in areas notable for long-term synaptic plasticity. (C) 2000 Wiley-Liss, Inc.

KW - Hippocampus

KW - Long-term potentiation (LTP)

KW - Protein kinase C zeta

KW - Protein kinase M zeta

KW - Purkinje cell

KW - Pyramidal cell

UR - http://www.scopus.com/inward/record.url?scp=85018132333&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85018132333&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1096-9861(20000103)416:1<79::AID-CNE6>3.0.CO;2-2

DO - 10.1002/(SICI)1096-9861(20000103)416:1<79::AID-CNE6>3.0.CO;2-2

M3 - Article

AN - SCOPUS:85018132333

VL - 426

SP - 243

EP - 258

JO - Journal of Comparative Neurology

JF - Journal of Comparative Neurology

SN - 0021-9967

IS - 2

ER -