Distinct signalling pathways coordinately contribute to virulence of Fusarium oxysporum on mammalian hosts

Rafael C. Prados-Rosales, Carolina Serena, Jesús Delgado-Jarana, Josep Guarro, Antonio Di Pietro

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The filamentous fungus Fusarium oxysporum causes vascular wilt on a wide range of plant species and is an emerging pathogen of humans. A mitogen-activated protein kinase, Fmk1, and a G protein β subunit, Fgb1, control pathogenicity of F. oxysporum on plants through distinct signalling pathways. In the present report, we studied the genetic interaction between fmk1 and fgb1 and their role in virulence on a mammalian host. The Δfmk1 or Δfgb1 single mutants exhibited similar virulence patterns as the wild type strain in an immunodepressed mouse model. By contrast, double mutants lacking both genes had dramatically reduced virulence. All mutants showed similar in vitro growth or tolerance to temperature and osmotic stress as the wild type strain. However, the Δfgb1 and Δfmk1 strains were reduced in specific extracellular protease activity or adhesion to fibronectin, respectively, two factors previously associated with fungal virulence. Thus, Fmk1 and Fgb1 are components of distinct signalling pathways which collectively control virulence of F. oxysporum on mammalian hosts.

Original languageEnglish (US)
Pages (from-to)2825-2831
Number of pages7
JournalMicrobes and Infection
Volume8
Issue number14-15
DOIs
StatePublished - Nov 2006
Externally publishedYes

Fingerprint

Fusarium
Virulence
Osmotic Pressure
Protein Subunits
Mitogen-Activated Protein Kinases
Fibronectins
GTP-Binding Proteins
Blood Vessels
Peptide Hydrolases
Fungi
Temperature
Growth
Genes

Keywords

  • Adhesion
  • cAMP
  • Fibronectin
  • G β subunit
  • Hydrophobicity
  • MAP kinase
  • Murine model
  • Protease

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

Prados-Rosales, R. C., Serena, C., Delgado-Jarana, J., Guarro, J., & Di Pietro, A. (2006). Distinct signalling pathways coordinately contribute to virulence of Fusarium oxysporum on mammalian hosts. Microbes and Infection, 8(14-15), 2825-2831. https://doi.org/10.1016/j.micinf.2006.08.015

Distinct signalling pathways coordinately contribute to virulence of Fusarium oxysporum on mammalian hosts. / Prados-Rosales, Rafael C.; Serena, Carolina; Delgado-Jarana, Jesús; Guarro, Josep; Di Pietro, Antonio.

In: Microbes and Infection, Vol. 8, No. 14-15, 11.2006, p. 2825-2831.

Research output: Contribution to journalArticle

Prados-Rosales, RC, Serena, C, Delgado-Jarana, J, Guarro, J & Di Pietro, A 2006, 'Distinct signalling pathways coordinately contribute to virulence of Fusarium oxysporum on mammalian hosts', Microbes and Infection, vol. 8, no. 14-15, pp. 2825-2831. https://doi.org/10.1016/j.micinf.2006.08.015
Prados-Rosales, Rafael C. ; Serena, Carolina ; Delgado-Jarana, Jesús ; Guarro, Josep ; Di Pietro, Antonio. / Distinct signalling pathways coordinately contribute to virulence of Fusarium oxysporum on mammalian hosts. In: Microbes and Infection. 2006 ; Vol. 8, No. 14-15. pp. 2825-2831.
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