Distinct lymphoblastic and myeloblastic populations in tdt positive acute myeloblastic leukemia: Evidence by double-fluorescence staining

Elisabeth M. Paietta, Peter Bettelheim, Josef D. Schwarzmeier, Dieter Lutz, Otto Majdic, Walter Knapp

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Double-immunofluorescent staining for the enzyme terminal deoxynucleotidyl transferase (TdT) as a marker of primitive lymphoblasts, and for the VIM-D5 antigen as a differentiation antigen of the myeloid system gave direct evidence for distinct lymphoblastic and myeloblastic populations (mixed leukemic cell populations) in seven patients with acute leukemia. The percentage of malignant TdT positive cells contributing to a leukemic cell bulk with unequivocal signs of myeloid origin was between 10 and 80%. A defect at the level of a common progenitor cell giving rise to both the TdT and the VIM-D5 positive blast cell population is discussed.

Original languageEnglish (US)
JournalLeukemia Research
Volume7
Issue number2
DOIs
StatePublished - 1983
Externally publishedYes

Fingerprint

Acute Myeloid Leukemia
DNA Nucleotidylexotransferase
Fluorescence
Staining and Labeling
Population
Differentiation Antigens
Leukemia
Stem Cells
Antigens
Enzymes

Keywords

  • Acute myeloblastic leukemia
  • double-fluorescence staining
  • terminal transferase

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

Cite this

Distinct lymphoblastic and myeloblastic populations in tdt positive acute myeloblastic leukemia : Evidence by double-fluorescence staining. / Paietta, Elisabeth M.; Bettelheim, Peter; Schwarzmeier, Josef D.; Lutz, Dieter; Majdic, Otto; Knapp, Walter.

In: Leukemia Research, Vol. 7, No. 2, 1983.

Research output: Contribution to journalArticle

Paietta, Elisabeth M. ; Bettelheim, Peter ; Schwarzmeier, Josef D. ; Lutz, Dieter ; Majdic, Otto ; Knapp, Walter. / Distinct lymphoblastic and myeloblastic populations in tdt positive acute myeloblastic leukemia : Evidence by double-fluorescence staining. In: Leukemia Research. 1983 ; Vol. 7, No. 2.
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