Distinct in vivo dendritic cell activation by live versus killed Listeria monocytogenes

Eric Muraille, Rielle Giannino, Patrick Guirnalda, Ingrid Leiner, Steffen Jung, Eric G. Pamer, Gregoire Lauvau

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Immunization of mice with live or heat-killed Listeria monocytogenes (HKLM) efficiently primes pathogen-specific CD8+ T cells. T lymphocytes primed by HKLM, however, undergo attenuated proliferation and do not fully differentiate. Thus, only infection with live bacteria induces long-term, CD8+ T cell-mediated protective immunity. In this study we demonstrate that live and heat-killed bacteria, while both associating with Mac-3+CD11bhi cells, localize to distinct splenic areas following intravenous inoculation. While HKLM localize to the marginal zone and the splenic red pulp, live L. monocytogenes are carried to the T cell zone of splenic white pulp. Despite these differences, in vivo depletion of CD11c-expressing cells prevents priming of naive T cells by either HKLM or live L. monocytogenes. Analysis of CD11chi dendritic cells (DC) reveals that infection with live L. monocytogenes induces higher levels of CD40, CD80 and CD86 expression than immunization with HKLM. Our results suggest that CD8+ T cell priming following HKLM immunization or live infection is mediated by DC and that the disparate outcomes of priming can be attributed to suboptimal conditioning of DC in the absence of live, cytosol-invasive bacteria.

Original languageEnglish (US)
Pages (from-to)1463-1471
Number of pages9
JournalEuropean Journal of Immunology
Volume35
Issue number5
DOIs
StatePublished - May 2005
Externally publishedYes

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Listeria monocytogenes
Dendritic Cells
Hot Temperature
T-Lymphocytes
Immunization
Bacteria
Infection
Cellular Immunity
Cytosol

Keywords

  • Bacterial infection
  • Costimulation
  • Dendritic cells
  • Memory CTL
  • Vaccination

ASJC Scopus subject areas

  • Immunology

Cite this

Distinct in vivo dendritic cell activation by live versus killed Listeria monocytogenes. / Muraille, Eric; Giannino, Rielle; Guirnalda, Patrick; Leiner, Ingrid; Jung, Steffen; Pamer, Eric G.; Lauvau, Gregoire.

In: European Journal of Immunology, Vol. 35, No. 5, 05.2005, p. 1463-1471.

Research output: Contribution to journalArticle

Muraille, E, Giannino, R, Guirnalda, P, Leiner, I, Jung, S, Pamer, EG & Lauvau, G 2005, 'Distinct in vivo dendritic cell activation by live versus killed Listeria monocytogenes', European Journal of Immunology, vol. 35, no. 5, pp. 1463-1471. https://doi.org/10.1002/eji.200526024
Muraille, Eric ; Giannino, Rielle ; Guirnalda, Patrick ; Leiner, Ingrid ; Jung, Steffen ; Pamer, Eric G. ; Lauvau, Gregoire. / Distinct in vivo dendritic cell activation by live versus killed Listeria monocytogenes. In: European Journal of Immunology. 2005 ; Vol. 35, No. 5. pp. 1463-1471.
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