Dissection of cis-regulatory elements in the C. elegans Hox gene egl-5 promoter

Yingqi Teng, Lisa Girard, Henrique B. Ferreira, Paul W. Sternberg, Scott W. Emmons

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Hox genes are highly conserved segmental identity genes well known for their complex expression patterns and divergent targets. Here we present an analysis of cis-regulatory elements in the Caenorhabditis elegans Hox gene egl-5, which is expressed in multiple tissues in the posterior region of the nematode. We have utilized phylogenetic footprinting to efficiently identify cis-regulatory elements and have characterized these with gfp reporters and tissue-specific rescue experiments. We have found that the complex expression pattern of egl-5 is the cumulative result of the activities of multiple tissue or local region-specific activator sequences that are conserved both in sequence and near-perfect order in the related nematode Caenorhabditis briggsae. Two conserved regulatory blocks analyzed in detail contain multiple sites for both positively and negatively acting factors. One of these regions may promote activation of egl-5 in certain cells via the Wnt pathway. Positively acting regions are repressed in inappropriate tissues by additional negative pathways acting at other sites within the promoter. Our analysis has allowed us to implicate several new regulatory factors significant to the control of egl-5 expression.

Original languageEnglish (US)
Pages (from-to)476-492
Number of pages17
JournalDevelopmental Biology
Volume276
Issue number2
DOIs
StatePublished - Dec 15 2004

Fingerprint

Homeobox Genes
Dissection
Caenorhabditis
Wnt Signaling Pathway
Conserved Sequence
Caenorhabditis elegans
Genes

Keywords

  • C. elegans
  • cis-Regulatory elements
  • egl-5
  • Hox
  • Phylogenetic footprinting

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Dissection of cis-regulatory elements in the C. elegans Hox gene egl-5 promoter. / Teng, Yingqi; Girard, Lisa; Ferreira, Henrique B.; Sternberg, Paul W.; Emmons, Scott W.

In: Developmental Biology, Vol. 276, No. 2, 15.12.2004, p. 476-492.

Research output: Contribution to journalArticle

Teng, Yingqi ; Girard, Lisa ; Ferreira, Henrique B. ; Sternberg, Paul W. ; Emmons, Scott W. / Dissection of cis-regulatory elements in the C. elegans Hox gene egl-5 promoter. In: Developmental Biology. 2004 ; Vol. 276, No. 2. pp. 476-492.
@article{e5a38a144d814017b833bb86996c379d,
title = "Dissection of cis-regulatory elements in the C. elegans Hox gene egl-5 promoter",
abstract = "Hox genes are highly conserved segmental identity genes well known for their complex expression patterns and divergent targets. Here we present an analysis of cis-regulatory elements in the Caenorhabditis elegans Hox gene egl-5, which is expressed in multiple tissues in the posterior region of the nematode. We have utilized phylogenetic footprinting to efficiently identify cis-regulatory elements and have characterized these with gfp reporters and tissue-specific rescue experiments. We have found that the complex expression pattern of egl-5 is the cumulative result of the activities of multiple tissue or local region-specific activator sequences that are conserved both in sequence and near-perfect order in the related nematode Caenorhabditis briggsae. Two conserved regulatory blocks analyzed in detail contain multiple sites for both positively and negatively acting factors. One of these regions may promote activation of egl-5 in certain cells via the Wnt pathway. Positively acting regions are repressed in inappropriate tissues by additional negative pathways acting at other sites within the promoter. Our analysis has allowed us to implicate several new regulatory factors significant to the control of egl-5 expression.",
keywords = "C. elegans, cis-Regulatory elements, egl-5, Hox, Phylogenetic footprinting",
author = "Yingqi Teng and Lisa Girard and Ferreira, {Henrique B.} and Sternberg, {Paul W.} and Emmons, {Scott W.}",
year = "2004",
month = "12",
day = "15",
doi = "10.1016/j.ydbio.2004.09.012",
language = "English (US)",
volume = "276",
pages = "476--492",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Dissection of cis-regulatory elements in the C. elegans Hox gene egl-5 promoter

AU - Teng, Yingqi

AU - Girard, Lisa

AU - Ferreira, Henrique B.

AU - Sternberg, Paul W.

AU - Emmons, Scott W.

PY - 2004/12/15

Y1 - 2004/12/15

N2 - Hox genes are highly conserved segmental identity genes well known for their complex expression patterns and divergent targets. Here we present an analysis of cis-regulatory elements in the Caenorhabditis elegans Hox gene egl-5, which is expressed in multiple tissues in the posterior region of the nematode. We have utilized phylogenetic footprinting to efficiently identify cis-regulatory elements and have characterized these with gfp reporters and tissue-specific rescue experiments. We have found that the complex expression pattern of egl-5 is the cumulative result of the activities of multiple tissue or local region-specific activator sequences that are conserved both in sequence and near-perfect order in the related nematode Caenorhabditis briggsae. Two conserved regulatory blocks analyzed in detail contain multiple sites for both positively and negatively acting factors. One of these regions may promote activation of egl-5 in certain cells via the Wnt pathway. Positively acting regions are repressed in inappropriate tissues by additional negative pathways acting at other sites within the promoter. Our analysis has allowed us to implicate several new regulatory factors significant to the control of egl-5 expression.

AB - Hox genes are highly conserved segmental identity genes well known for their complex expression patterns and divergent targets. Here we present an analysis of cis-regulatory elements in the Caenorhabditis elegans Hox gene egl-5, which is expressed in multiple tissues in the posterior region of the nematode. We have utilized phylogenetic footprinting to efficiently identify cis-regulatory elements and have characterized these with gfp reporters and tissue-specific rescue experiments. We have found that the complex expression pattern of egl-5 is the cumulative result of the activities of multiple tissue or local region-specific activator sequences that are conserved both in sequence and near-perfect order in the related nematode Caenorhabditis briggsae. Two conserved regulatory blocks analyzed in detail contain multiple sites for both positively and negatively acting factors. One of these regions may promote activation of egl-5 in certain cells via the Wnt pathway. Positively acting regions are repressed in inappropriate tissues by additional negative pathways acting at other sites within the promoter. Our analysis has allowed us to implicate several new regulatory factors significant to the control of egl-5 expression.

KW - C. elegans

KW - cis-Regulatory elements

KW - egl-5

KW - Hox

KW - Phylogenetic footprinting

UR - http://www.scopus.com/inward/record.url?scp=9944238960&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=9944238960&partnerID=8YFLogxK

U2 - 10.1016/j.ydbio.2004.09.012

DO - 10.1016/j.ydbio.2004.09.012

M3 - Article

C2 - 15581880

AN - SCOPUS:9944238960

VL - 276

SP - 476

EP - 492

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 2

ER -