Disruptive TP53 mutation is associated with aggressive disease characteristics in an orthotopic murine model of oral tongue cancer

Daisuke Sano, Tong Xin Xie, Thomas J. Ow, Mei Zhao, Curtis R. Pickering, Ge Zhou, Vlad C. Sandulache, David A. Wheeler, Richard A. Gibbs, Carlos Caulin, Jeffrey N. Myers

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Purpose: To characterize tumor growth and metastatic potential in head and neck squamous cell carcinoma (HNSCC) cell lines in an orthotopic murine model of oral tongue cancer and to correlate TP53 mutation status with these findings. Experimental Design: Cells from each of 48 HNSCC cell lines were orthotopically injected into the oral tongues of nude mice. Tumor volume, cervical lymph node metastasis, and mouse survival were recorded. Direct sequencing of the TP53 gene and Western blot analysis for the p53 protein after induction with 5-fluorouracil was conducted. Cell lines were categorized as either mutant TP53 or wild-type TP53, and lines with TP53 mutation were further categorized on the basis of type of mutation (disruptive or nondisruptive) and level of p53 protein expression. The behavior of tumors in these different groups was compared. Results: These 48 HNSCC cell lines showed a wide range of behavior from highly aggressive and metastatic to no tumor formation. Mice injected with cells harboring disruptive TP53 mutations had faster tumor growth, greater incidence of cervical lymph node metastasis, and shorter survival than mice injected with cells lacking these mutations. Conclusions: HNSCC cell lines display a wide spectrumof behavior in an orthotopicmodel of oral cancer. Cell lines with disruptive TP53 mutations are more aggressive in this system, corroborating clinical reports that have linked these mutations to poor patient outcome.

Original languageEnglish (US)
Pages (from-to)6658-6670
Number of pages13
JournalClinical Cancer Research
Volume17
Issue number21
DOIs
StatePublished - Nov 1 2011
Externally publishedYes

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Tongue Neoplasms
Mouth Neoplasms
Mutation
Cell Line
Neoplasms
Lymph Nodes
Neoplasm Metastasis
p53 Genes
Growth
Tumor Burden
Tongue
Nude Mice
Fluorouracil
Proteins
Research Design
Western Blotting
Survival
Carcinoma, squamous cell of head and neck
Incidence

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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Disruptive TP53 mutation is associated with aggressive disease characteristics in an orthotopic murine model of oral tongue cancer. / Sano, Daisuke; Xie, Tong Xin; Ow, Thomas J.; Zhao, Mei; Pickering, Curtis R.; Zhou, Ge; Sandulache, Vlad C.; Wheeler, David A.; Gibbs, Richard A.; Caulin, Carlos; Myers, Jeffrey N.

In: Clinical Cancer Research, Vol. 17, No. 21, 01.11.2011, p. 6658-6670.

Research output: Contribution to journalArticle

Sano, D, Xie, TX, Ow, TJ, Zhao, M, Pickering, CR, Zhou, G, Sandulache, VC, Wheeler, DA, Gibbs, RA, Caulin, C & Myers, JN 2011, 'Disruptive TP53 mutation is associated with aggressive disease characteristics in an orthotopic murine model of oral tongue cancer', Clinical Cancer Research, vol. 17, no. 21, pp. 6658-6670. https://doi.org/10.1158/1078-0432.CCR-11-0046
Sano, Daisuke ; Xie, Tong Xin ; Ow, Thomas J. ; Zhao, Mei ; Pickering, Curtis R. ; Zhou, Ge ; Sandulache, Vlad C. ; Wheeler, David A. ; Gibbs, Richard A. ; Caulin, Carlos ; Myers, Jeffrey N. / Disruptive TP53 mutation is associated with aggressive disease characteristics in an orthotopic murine model of oral tongue cancer. In: Clinical Cancer Research. 2011 ; Vol. 17, No. 21. pp. 6658-6670.
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