Discrimination of a colony stimulating factor subclass by a specific receptor on a macrophage cell line

S. K. Das, E. R. Stanley, L. J. Guilbert, L. W. Forman

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Utilizing the high affinity interactions between pure 125I-L cell colony stimulating factor and its receptor(s) on the murine macrophage cell line J774, a murine radioreceptor assay (RRA) has been developed. The murine RRA selectively detects a colony stimulating factor (CSF) subclass (CSF-1) previously defined by murine radioimmunoassay (RIA) (E.R. Stanley, Proc. Nat. Acad. Sci., USA, 76:2969-2973 ('79)). CSF-1 stimulates macrophage production exclusively, and the occurrence of the CSF-1 receptor(s) appears to be restricted to cells of the mononuclear phagocytic system (L.J. Guilbert and E.R. Stanley, J. Cell Biol. 85:153-160 ('80)). The murine CSF-1 RRA failed to detect a variety of other CSF subclasses, growth factors, and hormones. In contrast to data obtained with the murine CSF-1 RIA, human CSF-1 (e.g., human urinary CSF) is detected by the mouse CSF-1 RRA almost as sensitively as murine CSF-1. In addition, there was an absolute correlation between CSF-1 levels determined by murine CSF-1 RRA and those determined by a human CSF-1 RIA for a variety of human CSF-1 sources. The murine CSF-1 RRA is a sensitive (sensitivity 5 units or 1.0 femtomole of CSF-1 protein), rapid, and highly specific assay for CSF-1 in both murine and human sources.

Original languageEnglish (US)
Pages (from-to)359-366
Number of pages8
JournalJournal of Cellular Physiology
Volume104
Issue number3
StatePublished - 1980

Fingerprint

Colony-Stimulating Factors
Macrophage Colony-Stimulating Factor
Macrophages
Cells
Cell Line
Radioligand Assay
Assays
Colony-Stimulating Factor Receptors
Radioimmunoassay
Phagocytes

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Das, S. K., Stanley, E. R., Guilbert, L. J., & Forman, L. W. (1980). Discrimination of a colony stimulating factor subclass by a specific receptor on a macrophage cell line. Journal of Cellular Physiology, 104(3), 359-366.

Discrimination of a colony stimulating factor subclass by a specific receptor on a macrophage cell line. / Das, S. K.; Stanley, E. R.; Guilbert, L. J.; Forman, L. W.

In: Journal of Cellular Physiology, Vol. 104, No. 3, 1980, p. 359-366.

Research output: Contribution to journalArticle

Das, SK, Stanley, ER, Guilbert, LJ & Forman, LW 1980, 'Discrimination of a colony stimulating factor subclass by a specific receptor on a macrophage cell line', Journal of Cellular Physiology, vol. 104, no. 3, pp. 359-366.
Das, S. K. ; Stanley, E. R. ; Guilbert, L. J. ; Forman, L. W. / Discrimination of a colony stimulating factor subclass by a specific receptor on a macrophage cell line. In: Journal of Cellular Physiology. 1980 ; Vol. 104, No. 3. pp. 359-366.
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