Directed molecular evolution to design advanced red fluorescent proteins

Fedor V. Subach, Kiryl D. Piatkevich, Vladislav Verkhusha

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Fluorescent proteins have become indispensable imaging tools for biomedical research. Continuing progress in fluorescence imaging, however, requires probes with additional colors and properties optimized for emerging techniques. Here we summarize strategies for development of red-shifted fluorescent proteins. We discuss possibilities for knowledge-based rational design based on the photochemistry of fluorescent proteins and the position of the chromophore in protein structure. We consider advances in library design by mutagenesis, protein expression systems and instrumentation for high-throughput screening that should yield improved fluorescent proteins for advanced imaging applications.

Original languageEnglish (US)
Pages (from-to)1019-1026
Number of pages8
JournalNature Methods
Volume8
Issue number12
DOIs
StatePublished - Dec 2011

Fingerprint

Directed Molecular Evolution
Proteins
Imaging techniques
Photochemistry
Optical Imaging
Mutagenesis
Photochemical reactions
Libraries
Chromophores
Biomedical Research
Color
red fluorescent protein
Screening
Fluorescence
Throughput

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Directed molecular evolution to design advanced red fluorescent proteins. / Subach, Fedor V.; Piatkevich, Kiryl D.; Verkhusha, Vladislav.

In: Nature Methods, Vol. 8, No. 12, 12.2011, p. 1019-1026.

Research output: Contribution to journalArticle

Subach, Fedor V. ; Piatkevich, Kiryl D. ; Verkhusha, Vladislav. / Directed molecular evolution to design advanced red fluorescent proteins. In: Nature Methods. 2011 ; Vol. 8, No. 12. pp. 1019-1026.
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