Direct visualization of specifically modified extracellular glycans in living animals

Matthew Attreed; Muriel Desbois; Toin H. Van Kuppevelt; Hannes E. Bülow

doi:10.1038/nmeth.1945

Direct visualization of specifically modified extracellular glycans in living animals

Matthew Attreed, Muriel Desbois, Toin H. Van Kuppevelt, Hannes E. Bülow

Genetics

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Modification patterns of heparan sulfate coordinate protein function in metazoans, yet in vivo imaging of such non-genetically encoded structures has been impossible. Here we report a transgenic method in Caenorhabditis elegans that allows direct live imaging of specific heparan sulfate modification patterns. This experimental approach reveals a dynamic and cell-specific heparan sulfate landscape and could in principle be adapted to visualize and analyze any extracellular molecule in vivo.

Original language	English (US)
Pages (from-to)	477-479
Number of pages	3
Journal	Nature Methods
Volume	9
Issue number	5
DOIs	https://doi.org/10.1038/nmeth.1945
State	Published - May 1 2012

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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