Direct visualization of specifically modified extracellular glycans in living animals

Matthew Attreed, Muriel Desbois, Toin H. Van Kuppevelt, Hannes E. Bülow

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Modification patterns of heparan sulfate coordinate protein function in metazoans, yet in vivo imaging of such non-genetically encoded structures has been impossible. Here we report a transgenic method in Caenorhabditis elegans that allows direct live imaging of specific heparan sulfate modification patterns. This experimental approach reveals a dynamic and cell-specific heparan sulfate landscape and could in principle be adapted to visualize and analyze any extracellular molecule in vivo.

Original languageEnglish (US)
Pages (from-to)477-479
Number of pages3
JournalNature Methods
Volume9
Issue number5
DOIs
StatePublished - May 1 2012

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ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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