Direct visualization of specifically modified extracellular glycans in living animals

Matthew Attreed, Muriel Desbois, Toin H. Van Kuppevelt, Hannes E. Buelow

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Modification patterns of heparan sulfate coordinate protein function in metazoans, yet in vivo imaging of such non-genetically encoded structures has been impossible. Here we report a transgenic method in Caenorhabditis elegans that allows direct live imaging of specific heparan sulfate modification patterns. This experimental approach reveals a dynamic and cell-specific heparan sulfate landscape and could in principle be adapted to visualize and analyze any extracellular molecule in vivo.

Original languageEnglish (US)
Pages (from-to)477-479
Number of pages3
JournalNature Methods
Volume9
Issue number5
DOIs
StatePublished - May 2012

Fingerprint

Heparitin Sulfate
Polysaccharides
Animals
Visualization
Imaging techniques
Caenorhabditis elegans
Molecules
Proteins

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Direct visualization of specifically modified extracellular glycans in living animals. / Attreed, Matthew; Desbois, Muriel; Van Kuppevelt, Toin H.; Buelow, Hannes E.

In: Nature Methods, Vol. 9, No. 5, 05.2012, p. 477-479.

Research output: Contribution to journalArticle

Attreed, Matthew ; Desbois, Muriel ; Van Kuppevelt, Toin H. ; Buelow, Hannes E. / Direct visualization of specifically modified extracellular glycans in living animals. In: Nature Methods. 2012 ; Vol. 9, No. 5. pp. 477-479.
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