@article{661ef1eef69d474ea4a0acdbfe1c80d7,
title = "Direct visualization of specifically modified extracellular glycans in living animals",
abstract = "Modification patterns of heparan sulfate coordinate protein function in metazoans, yet in vivo imaging of such non-genetically encoded structures has been impossible. Here we report a transgenic method in Caenorhabditis elegans that allows direct live imaging of specific heparan sulfate modification patterns. This experimental approach reveals a dynamic and cell-specific heparan sulfate landscape and could in principle be adapted to visualize and analyze any extracellular molecule in vivo.",
author = "Matthew Attreed and Muriel Desbois and {Van Kuppevelt}, {Toin H.} and B{\"u}low, {Hannes E.}",
note = "Funding Information: We thank J. Attonito, N. Gomez and P. Weinberg for technical assistance, H. Fares (University of Arizona, Tucson) for providing the pJF33 plasmid, E. Snapp (Albert Einstein College of Medicine) for the superfolder GFP plasmid, S. Mitani (Tokyo Women{\textquoteright}s Medical University School of Medicine) for providing the tm734, tm3006 and tm3208 alleles of heparan 3O-sulfotransferases, and L. Attreed, S. Emmons, O. Hobert and members of the B{\"u}low laboratory for comments on the manuscript. This work was supported in part by grants from the US National Institutes of Health (5R01HD055380 and RC1GM090825 to H.E.B.; T32 GM007491 to M.A.). H.E.B. is funded as an Alfred P. Sloan fellow. Copyright: Copyright 2012 Elsevier B.V., All rights reserved.",
year = "2012",
month = may,
doi = "10.1038/nmeth.1945",
language = "English (US)",
volume = "9",
pages = "477--479",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Publishing Group",
number = "5",
}