Abstract
Modification patterns of heparan sulfate coordinate protein function in metazoans, yet in vivo imaging of such non-genetically encoded structures has been impossible. Here we report a transgenic method in Caenorhabditis elegans that allows direct live imaging of specific heparan sulfate modification patterns. This experimental approach reveals a dynamic and cell-specific heparan sulfate landscape and could in principle be adapted to visualize and analyze any extracellular molecule in vivo.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 477-479 |
| Number of pages | 3 |
| Journal | Nature Methods |
| Volume | 9 |
| Issue number | 5 |
| DOIs | |
| State | Published - May 2012 |
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ASJC Scopus subject areas
- Biotechnology
- Molecular Biology
- Biochemistry
- Cell Biology
Cite this
Direct visualization of specifically modified extracellular glycans in living animals. / Attreed, Matthew; Desbois, Muriel; Van Kuppevelt, Toin H.; Buelow, Hannes E.
In: Nature Methods, Vol. 9, No. 5, 05.2012, p. 477-479.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Direct visualization of specifically modified extracellular glycans in living animals
AU - Attreed, Matthew
AU - Desbois, Muriel
AU - Van Kuppevelt, Toin H.
AU - Buelow, Hannes E.
PY - 2012/5
Y1 - 2012/5
N2 - Modification patterns of heparan sulfate coordinate protein function in metazoans, yet in vivo imaging of such non-genetically encoded structures has been impossible. Here we report a transgenic method in Caenorhabditis elegans that allows direct live imaging of specific heparan sulfate modification patterns. This experimental approach reveals a dynamic and cell-specific heparan sulfate landscape and could in principle be adapted to visualize and analyze any extracellular molecule in vivo.
AB - Modification patterns of heparan sulfate coordinate protein function in metazoans, yet in vivo imaging of such non-genetically encoded structures has been impossible. Here we report a transgenic method in Caenorhabditis elegans that allows direct live imaging of specific heparan sulfate modification patterns. This experimental approach reveals a dynamic and cell-specific heparan sulfate landscape and could in principle be adapted to visualize and analyze any extracellular molecule in vivo.
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U2 - 10.1038/nmeth.1945
DO - 10.1038/nmeth.1945
M3 - Article
C2 - 22466794
AN - SCOPUS:84860374004
VL - 9
SP - 477
EP - 479
JO - Nature Methods
JF - Nature Methods
SN - 1548-7091
IS - 5
ER -