Abstract
Modification patterns of heparan sulfate coordinate protein function in metazoans, yet in vivo imaging of such non-genetically encoded structures has been impossible. Here we report a transgenic method in Caenorhabditis elegans that allows direct live imaging of specific heparan sulfate modification patterns. This experimental approach reveals a dynamic and cell-specific heparan sulfate landscape and could in principle be adapted to visualize and analyze any extracellular molecule in vivo.
Original language | English (US) |
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Pages (from-to) | 477-479 |
Number of pages | 3 |
Journal | Nature Methods |
Volume | 9 |
Issue number | 5 |
DOIs | |
State | Published - May 1 2012 |
ASJC Scopus subject areas
- Biotechnology
- Biochemistry
- Molecular Biology
- Cell Biology