Direct selection of trans-acting ligase ribozymes by in vitro compartmentalization

Matthew Levy, Karl E. Griswold, Andrew D. Ellington

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

We have used a compartmentalized in vitro selection method to directly select for ligase ribozymes that are capable of acting on and turning over separable oligonucleotide substrates. Starting from a degenerate pool, we selected a trans-acting variant of the Bartel class I ligase which statistically may have been the only active variant in the starting pool. The isolation of this sequence from the population suggests that this selection method is extremely robust at selecting optimal ribozymes and should, therefore, prove useful for the selection and optimization of other trans-acting nucleic acid catalysts capable of multiple turnover catalysis. Published by Cold Spring Harbor Laboratory Press.

Original languageEnglish (US)
Pages (from-to)1555-1562
Number of pages8
JournalRNA
Volume11
Issue number10
DOIs
StatePublished - Oct 2005
Externally publishedYes

Fingerprint

Catalytic RNA
Ligases
Catalysis
Oligonucleotides
Nucleic Acids
Population
In Vitro Techniques

Keywords

  • In vitro compartmentalization
  • In vitro selection
  • trans-acting ribozyme

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Direct selection of trans-acting ligase ribozymes by in vitro compartmentalization. / Levy, Matthew; Griswold, Karl E.; Ellington, Andrew D.

In: RNA, Vol. 11, No. 10, 10.2005, p. 1555-1562.

Research output: Contribution to journalArticle

Levy, M, Griswold, KE & Ellington, AD 2005, 'Direct selection of trans-acting ligase ribozymes by in vitro compartmentalization', RNA, vol. 11, no. 10, pp. 1555-1562. https://doi.org/10.1261/rna.2121705
Levy, Matthew ; Griswold, Karl E. ; Ellington, Andrew D. / Direct selection of trans-acting ligase ribozymes by in vitro compartmentalization. In: RNA. 2005 ; Vol. 11, No. 10. pp. 1555-1562.
@article{e6d8ef501c494f74bbecc1a9e64733ca,
title = "Direct selection of trans-acting ligase ribozymes by in vitro compartmentalization",
abstract = "We have used a compartmentalized in vitro selection method to directly select for ligase ribozymes that are capable of acting on and turning over separable oligonucleotide substrates. Starting from a degenerate pool, we selected a trans-acting variant of the Bartel class I ligase which statistically may have been the only active variant in the starting pool. The isolation of this sequence from the population suggests that this selection method is extremely robust at selecting optimal ribozymes and should, therefore, prove useful for the selection and optimization of other trans-acting nucleic acid catalysts capable of multiple turnover catalysis. Published by Cold Spring Harbor Laboratory Press.",
keywords = "In vitro compartmentalization, In vitro selection, trans-acting ribozyme",
author = "Matthew Levy and Griswold, {Karl E.} and Ellington, {Andrew D.}",
year = "2005",
month = "10",
doi = "10.1261/rna.2121705",
language = "English (US)",
volume = "11",
pages = "1555--1562",
journal = "RNA",
issn = "1355-8382",
publisher = "Cold Spring Harbor Laboratory Press",
number = "10",

}

TY - JOUR

T1 - Direct selection of trans-acting ligase ribozymes by in vitro compartmentalization

AU - Levy, Matthew

AU - Griswold, Karl E.

AU - Ellington, Andrew D.

PY - 2005/10

Y1 - 2005/10

N2 - We have used a compartmentalized in vitro selection method to directly select for ligase ribozymes that are capable of acting on and turning over separable oligonucleotide substrates. Starting from a degenerate pool, we selected a trans-acting variant of the Bartel class I ligase which statistically may have been the only active variant in the starting pool. The isolation of this sequence from the population suggests that this selection method is extremely robust at selecting optimal ribozymes and should, therefore, prove useful for the selection and optimization of other trans-acting nucleic acid catalysts capable of multiple turnover catalysis. Published by Cold Spring Harbor Laboratory Press.

AB - We have used a compartmentalized in vitro selection method to directly select for ligase ribozymes that are capable of acting on and turning over separable oligonucleotide substrates. Starting from a degenerate pool, we selected a trans-acting variant of the Bartel class I ligase which statistically may have been the only active variant in the starting pool. The isolation of this sequence from the population suggests that this selection method is extremely robust at selecting optimal ribozymes and should, therefore, prove useful for the selection and optimization of other trans-acting nucleic acid catalysts capable of multiple turnover catalysis. Published by Cold Spring Harbor Laboratory Press.

KW - In vitro compartmentalization

KW - In vitro selection

KW - trans-acting ribozyme

UR - http://www.scopus.com/inward/record.url?scp=25844472658&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=25844472658&partnerID=8YFLogxK

U2 - 10.1261/rna.2121705

DO - 10.1261/rna.2121705

M3 - Article

VL - 11

SP - 1555

EP - 1562

JO - RNA

JF - RNA

SN - 1355-8382

IS - 10

ER -