Direct selection of trans-acting ligase ribozymes by in vitro compartmentalization

Matthew Levy, Karl E. Griswold, Andrew D. Ellington

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

We have used a compartmentalized in vitro selection method to directly select for ligase ribozymes that are capable of acting on and turning over separable oligonucleotide substrates. Starting from a degenerate pool, we selected a trans-acting variant of the Bartel class I ligase which statistically may have been the only active variant in the starting pool. The isolation of this sequence from the population suggests that this selection method is extremely robust at selecting optimal ribozymes and should, therefore, prove useful for the selection and optimization of other trans-acting nucleic acid catalysts capable of multiple turnover catalysis. Published by Cold Spring Harbor Laboratory Press.

Original languageEnglish (US)
Pages (from-to)1555-1562
Number of pages8
JournalRNA
Volume11
Issue number10
DOIs
StatePublished - Oct 1 2005

Keywords

  • In vitro compartmentalization
  • In vitro selection
  • trans-acting ribozyme

ASJC Scopus subject areas

  • Molecular Biology

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