TY - JOUR
T1 - Direct identification of Staphylococcus aureus from positive blood culture bottles
AU - Oliveira, Kenneth
AU - Brecher, Stephen M.
AU - Durbin, Annette
AU - Shapiro, Daniel S.
AU - Schwartz, Donald R.
AU - De Girolami, Paola C.
AU - Dakos, Joanna
AU - Procop, Gary W.
AU - Wilson, Deborah
AU - Hanna, Chad S.
AU - Haase, Gerhard
AU - Peltroche-Llacsahuanga, Heidrun
AU - Chapin, Kimberle C.
AU - Musgnug, Michael C.
AU - Levi, Michael H.
AU - Shoemaker, Cynthia
AU - Stender, Henrik
PY - 2003/2/1
Y1 - 2003/2/1
N2 - Fluorescence in situ hybridization (FISH) using peptide nucleic acid (PNA) probes targeting Staphylococcus aureus 16S rRNA is a novel method for direct identification of S. aureus from positive blood culture bottles. The test (S. aureus PNA FISH) is performed on smears made directly from positive blood culture bottles with gram-positive cocci in clusters (GPCC) and provides results within 2.5 h. A blinded comparison of S. aureus PNA FISH with standard identification methods was performed in collaboration with eight clinical microbiology laboratories. A total of 564 routine blood culture bottles positive for GPCC recovered from both aerobic and anaerobic media from three different manufacturers (ESP, BACTEC, and BacT/Alert) were included in the study. The sensitivity and specificity of S. aureus PNA FISH were 100% (57 of 57) and 99.2% (116 of 117), respectively, with 174 GPCC-positive ESP blood culture bottles, 98.5% (67 of 68) and 98.5% (129 of 131), respectively, with 200 GPCC-positive BACTEC blood culture bottles, and 100% (74 of 74) and 99.1% (115 of 116), respectively, with 190 GPCC-positive BacT/Alert blood culture bottles. It is concluded that S. aureus PNA FISH performs well with commonly used continuously monitoring blood culture systems.
AB - Fluorescence in situ hybridization (FISH) using peptide nucleic acid (PNA) probes targeting Staphylococcus aureus 16S rRNA is a novel method for direct identification of S. aureus from positive blood culture bottles. The test (S. aureus PNA FISH) is performed on smears made directly from positive blood culture bottles with gram-positive cocci in clusters (GPCC) and provides results within 2.5 h. A blinded comparison of S. aureus PNA FISH with standard identification methods was performed in collaboration with eight clinical microbiology laboratories. A total of 564 routine blood culture bottles positive for GPCC recovered from both aerobic and anaerobic media from three different manufacturers (ESP, BACTEC, and BacT/Alert) were included in the study. The sensitivity and specificity of S. aureus PNA FISH were 100% (57 of 57) and 99.2% (116 of 117), respectively, with 174 GPCC-positive ESP blood culture bottles, 98.5% (67 of 68) and 98.5% (129 of 131), respectively, with 200 GPCC-positive BACTEC blood culture bottles, and 100% (74 of 74) and 99.1% (115 of 116), respectively, with 190 GPCC-positive BacT/Alert blood culture bottles. It is concluded that S. aureus PNA FISH performs well with commonly used continuously monitoring blood culture systems.
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U2 - 10.1128/JCM.41.2.889-891.2003
DO - 10.1128/JCM.41.2.889-891.2003
M3 - Article
C2 - 12574309
AN - SCOPUS:0037312212
SN - 0095-1137
VL - 41
SP - 889
EP - 891
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 2
ER -