Direct human papillomavirus E6 whole-cell enzyme-linked immunosorbent assay for objective measurement of E6 oncoproteins in cytology samples

Yi Shan Yang, Karen Smith-McCune, Teresa M. Darragh, Yvonne Lai, Ju Hwa Lin, Ting Chang Chang, Hsiao Yun Guo, Tiea Kesler, Alicia Carter, Philip E. Castle, Shuling Cheng

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A novel, whole-cell enzyme-linked immunosorbent assay (ELISA) based on a non-type-specific anti-human papillomavirus (HPV) E6 antibody was tested on 182 residual cytological specimens. For samples with a designation of more severe than cervical intraepithelial neoplasia grade 3 (CIN3+), 83% tested positive for E6; in a subset with paired testing for E6 ELISA and HPV DNA, 72% tested E6 positive and 92% tested high-risk (HR)-HPV DNA positive (P=0.2). Among the women with a less than CIN3 diagnosis, 31% and 47% tested positive for E6 and HR-HPV DNA, respectively (P=0.0006).

Original languageEnglish (US)
Pages (from-to)1474-1479
Number of pages6
JournalClinical and Vaccine Immunology
Volume19
Issue number9
DOIs
StatePublished - Sep 2012
Externally publishedYes

Fingerprint

Cytology
Immunosorbents
Oncogene Proteins
Cell Biology
Assays
Enzyme-Linked Immunosorbent Assay
DNA
Enzymes
Cervical Intraepithelial Neoplasia
Antibodies
Testing

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Immunology
  • Immunology and Allergy
  • Microbiology (medical)

Cite this

Direct human papillomavirus E6 whole-cell enzyme-linked immunosorbent assay for objective measurement of E6 oncoproteins in cytology samples. / Yang, Yi Shan; Smith-McCune, Karen; Darragh, Teresa M.; Lai, Yvonne; Lin, Ju Hwa; Chang, Ting Chang; Guo, Hsiao Yun; Kesler, Tiea; Carter, Alicia; Castle, Philip E.; Cheng, Shuling.

In: Clinical and Vaccine Immunology, Vol. 19, No. 9, 09.2012, p. 1474-1479.

Research output: Contribution to journalArticle

Yang, YS, Smith-McCune, K, Darragh, TM, Lai, Y, Lin, JH, Chang, TC, Guo, HY, Kesler, T, Carter, A, Castle, PE & Cheng, S 2012, 'Direct human papillomavirus E6 whole-cell enzyme-linked immunosorbent assay for objective measurement of E6 oncoproteins in cytology samples', Clinical and Vaccine Immunology, vol. 19, no. 9, pp. 1474-1479. https://doi.org/10.1128/CVI.00388-12
Yang, Yi Shan ; Smith-McCune, Karen ; Darragh, Teresa M. ; Lai, Yvonne ; Lin, Ju Hwa ; Chang, Ting Chang ; Guo, Hsiao Yun ; Kesler, Tiea ; Carter, Alicia ; Castle, Philip E. ; Cheng, Shuling. / Direct human papillomavirus E6 whole-cell enzyme-linked immunosorbent assay for objective measurement of E6 oncoproteins in cytology samples. In: Clinical and Vaccine Immunology. 2012 ; Vol. 19, No. 9. pp. 1474-1479.
@article{2358959f4f6d4c2ea20c6134c20e0cf3,
title = "Direct human papillomavirus E6 whole-cell enzyme-linked immunosorbent assay for objective measurement of E6 oncoproteins in cytology samples",
abstract = "A novel, whole-cell enzyme-linked immunosorbent assay (ELISA) based on a non-type-specific anti-human papillomavirus (HPV) E6 antibody was tested on 182 residual cytological specimens. For samples with a designation of more severe than cervical intraepithelial neoplasia grade 3 (CIN3+), 83{\%} tested positive for E6; in a subset with paired testing for E6 ELISA and HPV DNA, 72{\%} tested E6 positive and 92{\%} tested high-risk (HR)-HPV DNA positive (P=0.2). Among the women with a less than CIN3 diagnosis, 31{\%} and 47{\%} tested positive for E6 and HR-HPV DNA, respectively (P=0.0006).",
author = "Yang, {Yi Shan} and Karen Smith-McCune and Darragh, {Teresa M.} and Yvonne Lai and Lin, {Ju Hwa} and Chang, {Ting Chang} and Guo, {Hsiao Yun} and Tiea Kesler and Alicia Carter and Castle, {Philip E.} and Shuling Cheng",
year = "2012",
month = "9",
doi = "10.1128/CVI.00388-12",
language = "English (US)",
volume = "19",
pages = "1474--1479",
journal = "Clinical and Vaccine Immunology",
issn = "1556-6811",
publisher = "American Society for Microbiology",
number = "9",

}

TY - JOUR

T1 - Direct human papillomavirus E6 whole-cell enzyme-linked immunosorbent assay for objective measurement of E6 oncoproteins in cytology samples

AU - Yang, Yi Shan

AU - Smith-McCune, Karen

AU - Darragh, Teresa M.

AU - Lai, Yvonne

AU - Lin, Ju Hwa

AU - Chang, Ting Chang

AU - Guo, Hsiao Yun

AU - Kesler, Tiea

AU - Carter, Alicia

AU - Castle, Philip E.

AU - Cheng, Shuling

PY - 2012/9

Y1 - 2012/9

N2 - A novel, whole-cell enzyme-linked immunosorbent assay (ELISA) based on a non-type-specific anti-human papillomavirus (HPV) E6 antibody was tested on 182 residual cytological specimens. For samples with a designation of more severe than cervical intraepithelial neoplasia grade 3 (CIN3+), 83% tested positive for E6; in a subset with paired testing for E6 ELISA and HPV DNA, 72% tested E6 positive and 92% tested high-risk (HR)-HPV DNA positive (P=0.2). Among the women with a less than CIN3 diagnosis, 31% and 47% tested positive for E6 and HR-HPV DNA, respectively (P=0.0006).

AB - A novel, whole-cell enzyme-linked immunosorbent assay (ELISA) based on a non-type-specific anti-human papillomavirus (HPV) E6 antibody was tested on 182 residual cytological specimens. For samples with a designation of more severe than cervical intraepithelial neoplasia grade 3 (CIN3+), 83% tested positive for E6; in a subset with paired testing for E6 ELISA and HPV DNA, 72% tested E6 positive and 92% tested high-risk (HR)-HPV DNA positive (P=0.2). Among the women with a less than CIN3 diagnosis, 31% and 47% tested positive for E6 and HR-HPV DNA, respectively (P=0.0006).

UR - http://www.scopus.com/inward/record.url?scp=84866004230&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84866004230&partnerID=8YFLogxK

U2 - 10.1128/CVI.00388-12

DO - 10.1128/CVI.00388-12

M3 - Article

C2 - 22815148

AN - SCOPUS:84866004230

VL - 19

SP - 1474

EP - 1479

JO - Clinical and Vaccine Immunology

JF - Clinical and Vaccine Immunology

SN - 1556-6811

IS - 9

ER -