Direct human papillomavirus E6 whole-cell enzyme-linked immunosorbent assay for objective measurement of E6 oncoproteins in cytology samples

Yi Shan Yang, Karen Smith-McCune, Teresa M. Darragh, Yvonne Lai, Ju Hwa Lin, Ting Chang Chang, Hsiao Yun Guo, Tiea Kesler, Alicia Carter, Philip E. Castle, Shuling Cheng

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

A novel, whole-cell enzyme-linked immunosorbent assay (ELISA) based on a non-type-specific anti-human papillomavirus (HPV) E6 antibody was tested on 182 residual cytological specimens. For samples with a designation of more severe than cervical intraepithelial neoplasia grade 3 (CIN3+), 83% tested positive for E6; in a subset with paired testing for E6 ELISA and HPV DNA, 72% tested E6 positive and 92% tested high-risk (HR)-HPV DNA positive (P=0.2). Among the women with a less than CIN3 diagnosis, 31% and 47% tested positive for E6 and HR-HPV DNA, respectively (P=0.0006).

Original languageEnglish (US)
Pages (from-to)1474-1479
Number of pages6
JournalClinical and Vaccine Immunology
Volume19
Issue number9
DOIs
StatePublished - Sep 2012

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

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    Yang, Y. S., Smith-McCune, K., Darragh, T. M., Lai, Y., Lin, J. H., Chang, T. C., Guo, H. Y., Kesler, T., Carter, A., Castle, P. E., & Cheng, S. (2012). Direct human papillomavirus E6 whole-cell enzyme-linked immunosorbent assay for objective measurement of E6 oncoproteins in cytology samples. Clinical and Vaccine Immunology, 19(9), 1474-1479. https://doi.org/10.1128/CVI.00388-12