Direct, genome-wide assessment of DNA mutations in single cells

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

DNA mutations are the inevitable consequences of errors that arise during replication and repair of DNA damage. Because of their random and infrequent occurrence, quantification and characterization of DNA mutations in the genome of somatic cells has been difficult. Random, low-abundance mutations are currently inaccessible by standard high-throughput sequencing approaches because they cannot be distinguished from sequencing errors. One way to circumvent this problem and simultaneously account for the mutational heterogeneity within tissues is whole genome sequencing of a representative number of single cells. Here, we show elevated mutation levels in single cells from Drosophila melanogaster S2 and mouse embryonic fibroblast populations after treatment with the powerful mutagen N-ethyl-N-nitrosourea. This method can be applied as a direct measure of exposure to mutagenic agents and for assessing genotypic heterogeneity within tissues or cell populations.

Original languageEnglish (US)
Pages (from-to)2032-2040
Number of pages9
JournalNucleic acids research
Volume40
Issue number5
DOIs
StatePublished - Mar 1 2012

ASJC Scopus subject areas

  • Genetics

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