TY - JOUR
T1 - Direct electrochemistry of horseradish peroxidase based on biocompatible carboxymethyl chitosan-gold nanoparticle nanocomposite
AU - Xu, Qin
AU - Mao, Chun
AU - Liu, Ni Na
AU - Zhu, Jun Jie
AU - Sheng, Jian
N1 - Funding Information:
This work is supported by the National Natural Science Foundation of China (Grant Nos. 20325516 and 20575026), the Foundation of State Key state Laboratory of Electroanalytical Chemistry from Changchun Institute of Applied Chemistry and Jiangsu Scientific Project (BK2004210).
PY - 2006/12/15
Y1 - 2006/12/15
N2 - The nanocomposite composed of carboxymethyl chitosan (CMCS) and gold nanoparticles was successfully prepared by a novel and in situ process. It was characterized by transmission electron microscopy (TEM) and Fourier transform infrared spectrophotometer (FTIR). The nanocomposite was hydrophilic even in neutral solutions, stable and inherited the properties of the AuNPs and CMCS, which make it biocompatible for enzymes immobilization. HRP, as a model enzyme, was immobilized on the silica sol-gel matrix containing the nanocomposite to construct a novel H2O2 biosensor. The direct electron transfer of HRP was achieved and investigated. The biosensor exhibited a fast amperometric response (5 s), a good linear response over a wide range of concentrations from 5.0 × 10-6 to 1.4 × 10-3 M, and a low detection limit of 4.01 × 10-7 M. The apparent Michaelis-Menten constant (KMapp) for the biosensor was 5.7 × 10-4 M. Good stability and sensitivity were assessed for the biosensor.
AB - The nanocomposite composed of carboxymethyl chitosan (CMCS) and gold nanoparticles was successfully prepared by a novel and in situ process. It was characterized by transmission electron microscopy (TEM) and Fourier transform infrared spectrophotometer (FTIR). The nanocomposite was hydrophilic even in neutral solutions, stable and inherited the properties of the AuNPs and CMCS, which make it biocompatible for enzymes immobilization. HRP, as a model enzyme, was immobilized on the silica sol-gel matrix containing the nanocomposite to construct a novel H2O2 biosensor. The direct electron transfer of HRP was achieved and investigated. The biosensor exhibited a fast amperometric response (5 s), a good linear response over a wide range of concentrations from 5.0 × 10-6 to 1.4 × 10-3 M, and a low detection limit of 4.01 × 10-7 M. The apparent Michaelis-Menten constant (KMapp) for the biosensor was 5.7 × 10-4 M. Good stability and sensitivity were assessed for the biosensor.
KW - Biocompatibility
KW - Direct electrochemistry
KW - Nanocomposite
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U2 - 10.1016/j.bios.2006.02.010
DO - 10.1016/j.bios.2006.02.010
M3 - Article
C2 - 16600589
AN - SCOPUS:33750299566
SN - 0956-5663
VL - 22
SP - 768
EP - 773
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
IS - 5
ER -