Direct electrochemistry of horseradish peroxidase based on biocompatible carboxymethyl chitosan-gold nanoparticle nanocomposite

Qin Xu; Chun Mao; Ni Na Liu; Jun Jie Zhu; Jian Sheng

doi:10.1016/j.bios.2006.02.010

Direct electrochemistry of horseradish peroxidase based on biocompatible carboxymethyl chitosan-gold nanoparticle nanocomposite

Qin Xu, Chun Mao, Ni Na Liu, Jun Jie Zhu, Jian Sheng

Research output: Contribution to journal › Article › peer-review

241 Scopus citations

Abstract

The nanocomposite composed of carboxymethyl chitosan (CMCS) and gold nanoparticles was successfully prepared by a novel and in situ process. It was characterized by transmission electron microscopy (TEM) and Fourier transform infrared spectrophotometer (FTIR). The nanocomposite was hydrophilic even in neutral solutions, stable and inherited the properties of the AuNPs and CMCS, which make it biocompatible for enzymes immobilization. HRP, as a model enzyme, was immobilized on the silica sol-gel matrix containing the nanocomposite to construct a novel H₂O₂ biosensor. The direct electron transfer of HRP was achieved and investigated. The biosensor exhibited a fast amperometric response (5 s), a good linear response over a wide range of concentrations from 5.0 × 10^-6 to 1.4 × 10^-3 M, and a low detection limit of 4.01 × 10^-7 M. The apparent Michaelis-Menten constant (K_M^app) for the biosensor was 5.7 × 10^-4 M. Good stability and sensitivity were assessed for the biosensor.

Original language	English (US)
Pages (from-to)	768-773
Number of pages	6
Journal	Biosensors and Bioelectronics
Volume	22
Issue number	5
DOIs	https://doi.org/10.1016/j.bios.2006.02.010
State	Published - Dec 15 2006
Externally published	Yes

Keywords

Biocompatibility
Direct electrochemistry
Nanocomposite

ASJC Scopus subject areas

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

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10.1016/j.bios.2006.02.010

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title = "Direct electrochemistry of horseradish peroxidase based on biocompatible carboxymethyl chitosan-gold nanoparticle nanocomposite",

abstract = "The nanocomposite composed of carboxymethyl chitosan (CMCS) and gold nanoparticles was successfully prepared by a novel and in situ process. It was characterized by transmission electron microscopy (TEM) and Fourier transform infrared spectrophotometer (FTIR). The nanocomposite was hydrophilic even in neutral solutions, stable and inherited the properties of the AuNPs and CMCS, which make it biocompatible for enzymes immobilization. HRP, as a model enzyme, was immobilized on the silica sol-gel matrix containing the nanocomposite to construct a novel H2O2 biosensor. The direct electron transfer of HRP was achieved and investigated. The biosensor exhibited a fast amperometric response (5 s), a good linear response over a wide range of concentrations from 5.0 × 10-6 to 1.4 × 10-3 M, and a low detection limit of 4.01 × 10-7 M. The apparent Michaelis-Menten constant (KMapp) for the biosensor was 5.7 × 10-4 M. Good stability and sensitivity were assessed for the biosensor.",

keywords = "Biocompatibility, Direct electrochemistry, Nanocomposite",

author = "Qin Xu and Chun Mao and Liu, {Ni Na} and Zhu, {Jun Jie} and Jian Sheng",

note = "Funding Information: This work is supported by the National Natural Science Foundation of China (Grant Nos. 20325516 and 20575026), the Foundation of State Key state Laboratory of Electroanalytical Chemistry from Changchun Institute of Applied Chemistry and Jiangsu Scientific Project (BK2004210).",

year = "2006",

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doi = "10.1016/j.bios.2006.02.010",

language = "English (US)",

volume = "22",

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T1 - Direct electrochemistry of horseradish peroxidase based on biocompatible carboxymethyl chitosan-gold nanoparticle nanocomposite

AU - Xu, Qin

AU - Mao, Chun

AU - Liu, Ni Na

AU - Zhu, Jun Jie

AU - Sheng, Jian

N1 - Funding Information: This work is supported by the National Natural Science Foundation of China (Grant Nos. 20325516 and 20575026), the Foundation of State Key state Laboratory of Electroanalytical Chemistry from Changchun Institute of Applied Chemistry and Jiangsu Scientific Project (BK2004210).

PY - 2006/12/15

Y1 - 2006/12/15

N2 - The nanocomposite composed of carboxymethyl chitosan (CMCS) and gold nanoparticles was successfully prepared by a novel and in situ process. It was characterized by transmission electron microscopy (TEM) and Fourier transform infrared spectrophotometer (FTIR). The nanocomposite was hydrophilic even in neutral solutions, stable and inherited the properties of the AuNPs and CMCS, which make it biocompatible for enzymes immobilization. HRP, as a model enzyme, was immobilized on the silica sol-gel matrix containing the nanocomposite to construct a novel H2O2 biosensor. The direct electron transfer of HRP was achieved and investigated. The biosensor exhibited a fast amperometric response (5 s), a good linear response over a wide range of concentrations from 5.0 × 10-6 to 1.4 × 10-3 M, and a low detection limit of 4.01 × 10-7 M. The apparent Michaelis-Menten constant (KMapp) for the biosensor was 5.7 × 10-4 M. Good stability and sensitivity were assessed for the biosensor.

AB - The nanocomposite composed of carboxymethyl chitosan (CMCS) and gold nanoparticles was successfully prepared by a novel and in situ process. It was characterized by transmission electron microscopy (TEM) and Fourier transform infrared spectrophotometer (FTIR). The nanocomposite was hydrophilic even in neutral solutions, stable and inherited the properties of the AuNPs and CMCS, which make it biocompatible for enzymes immobilization. HRP, as a model enzyme, was immobilized on the silica sol-gel matrix containing the nanocomposite to construct a novel H2O2 biosensor. The direct electron transfer of HRP was achieved and investigated. The biosensor exhibited a fast amperometric response (5 s), a good linear response over a wide range of concentrations from 5.0 × 10-6 to 1.4 × 10-3 M, and a low detection limit of 4.01 × 10-7 M. The apparent Michaelis-Menten constant (KMapp) for the biosensor was 5.7 × 10-4 M. Good stability and sensitivity were assessed for the biosensor.

KW - Biocompatibility

KW - Direct electrochemistry

KW - Nanocomposite

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Direct electrochemistry of horseradish peroxidase based on biocompatible carboxymethyl chitosan-gold nanoparticle nanocomposite

Abstract

Keywords

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