Direct electrochemistry of horseradish peroxidase based on biocompatible carboxymethyl chitosan-gold nanoparticle nanocomposite

Qin Xu, Chun Mao, Ni Na Liu, Jun Jie Zhu, Jian Sheng

Research output: Contribution to journalArticle

199 Citations (Scopus)

Abstract

The nanocomposite composed of carboxymethyl chitosan (CMCS) and gold nanoparticles was successfully prepared by a novel and in situ process. It was characterized by transmission electron microscopy (TEM) and Fourier transform infrared spectrophotometer (FTIR). The nanocomposite was hydrophilic even in neutral solutions, stable and inherited the properties of the AuNPs and CMCS, which make it biocompatible for enzymes immobilization. HRP, as a model enzyme, was immobilized on the silica sol-gel matrix containing the nanocomposite to construct a novel H2O2 biosensor. The direct electron transfer of HRP was achieved and investigated. The biosensor exhibited a fast amperometric response (5 s), a good linear response over a wide range of concentrations from 5.0 × 10-6 to 1.4 × 10-3 M, and a low detection limit of 4.01 × 10-7 M. The apparent Michaelis-Menten constant (KM app) for the biosensor was 5.7 × 10-4 M. Good stability and sensitivity were assessed for the biosensor.

Original languageEnglish (US)
Pages (from-to)768-773
Number of pages6
JournalBiosensors and Bioelectronics
Volume22
Issue number5
DOIs
StatePublished - Dec 15 2006
Externally publishedYes

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Electrochemistry
Nanocomposites
Biosensing Techniques
Horseradish Peroxidase
Chitosan
Biosensors
Gold
Nanoparticles
Infrared spectrophotometers
Enzyme immobilization
Immobilized Enzymes
Silica Gel
Polymethyl Methacrylate
Fourier Analysis
Transmission Electron Microscopy
Silicon Dioxide
Immobilization
Sol-gels
Limit of Detection
Fourier transforms

Keywords

  • Biocompatibility
  • Direct electrochemistry
  • Nanocomposite

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Electrochemistry

Cite this

Direct electrochemistry of horseradish peroxidase based on biocompatible carboxymethyl chitosan-gold nanoparticle nanocomposite. / Xu, Qin; Mao, Chun; Liu, Ni Na; Zhu, Jun Jie; Sheng, Jian.

In: Biosensors and Bioelectronics, Vol. 22, No. 5, 15.12.2006, p. 768-773.

Research output: Contribution to journalArticle

Xu, Qin ; Mao, Chun ; Liu, Ni Na ; Zhu, Jun Jie ; Sheng, Jian. / Direct electrochemistry of horseradish peroxidase based on biocompatible carboxymethyl chitosan-gold nanoparticle nanocomposite. In: Biosensors and Bioelectronics. 2006 ; Vol. 22, No. 5. pp. 768-773.
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