Direct determination of human urinary cortisol metabolites by HPLC CRIMS

Alfred L. Yergey, Yohannes Teffera, Nora V. Esteban, Fred P. Abramson

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Feasibility of using high performance liquid chromatographic input to the chemical reaction interface mass spectrometry system was assessed by measuring the profile of hydrolyzed urinary metabolites of [9,12,12-2H3]cortisol in six human subjects with no preparation other than hydrolysis and solid phase extraction. Relative amounts of tetrahydrocortisol, tetrahydrocortisone, and cortolones (as the sum of α- and β-) were 0.417 ± 0.047, 0.523 ± 0.036 and 0.059 ± 0.019, respectively. The constant reproducibility of the measurements coupled with a profile consistent with that observed by other workers shows that the technique represents an important tool in the determination of metabolites of endogenous molecules.

Original languageEnglish (US)
Pages (from-to)295-298
Number of pages4
JournalSteroids
Volume60
Issue number3
DOIs
StatePublished - Mar 1995

Keywords

  • CRIMS
  • HPLC
  • cortisol
  • mass spectrometry
  • metabolites

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology
  • Pharmacology
  • Clinical Biochemistry
  • Organic Chemistry

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    Yergey, A. L., Teffera, Y., Esteban, N. V., & Abramson, F. P. (1995). Direct determination of human urinary cortisol metabolites by HPLC CRIMS. Steroids, 60(3), 295-298. https://doi.org/10.1016/0039-128X(94)00071-J