Direct determination of human urinary cortisol metabolites by HPLC CRIMS

Alfred L. Yergey, Yohannes Teffera, Nora V. Esteban, Fred P. Abramson

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Feasibility of using high performance liquid chromatographic input to the chemical reaction interface mass spectrometry system was assessed by measuring the profile of hydrolyzed urinary metabolites of [9,12,12-2H3]cortisol in six human subjects with no preparation other than hydrolysis and solid phase extraction. Relative amounts of tetrahydrocortisol, tetrahydrocortisone, and cortolones (as the sum of α- and β-) were 0.417 ± 0.047, 0.523 ± 0.036 and 0.059 ± 0.019, respectively. The constant reproducibility of the measurements coupled with a profile consistent with that observed by other workers shows that the technique represents an important tool in the determination of metabolites of endogenous molecules.

Original languageEnglish (US)
Pages (from-to)295-298
Number of pages4
JournalSteroids
Volume60
Issue number3
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

Tetrahydrocortisone
Tetrahydrocortisol
Solid Phase Extraction
Metabolites
Hydrocortisone
Mass Spectrometry
Hydrolysis
High Pressure Liquid Chromatography
Mass spectrometry
Chemical reactions
Molecules
Liquids
cortolone

Keywords

  • cortisol
  • CRIMS
  • HPLC
  • mass spectrometry
  • metabolites

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Endocrinology
  • Molecular Biology
  • Organic Chemistry
  • Pharmacology

Cite this

Yergey, A. L., Teffera, Y., Esteban, N. V., & Abramson, F. P. (1995). Direct determination of human urinary cortisol metabolites by HPLC CRIMS. Steroids, 60(3), 295-298. https://doi.org/10.1016/0039-128X(94)00071-J

Direct determination of human urinary cortisol metabolites by HPLC CRIMS. / Yergey, Alfred L.; Teffera, Yohannes; Esteban, Nora V.; Abramson, Fred P.

In: Steroids, Vol. 60, No. 3, 1995, p. 295-298.

Research output: Contribution to journalArticle

Yergey, AL, Teffera, Y, Esteban, NV & Abramson, FP 1995, 'Direct determination of human urinary cortisol metabolites by HPLC CRIMS', Steroids, vol. 60, no. 3, pp. 295-298. https://doi.org/10.1016/0039-128X(94)00071-J
Yergey, Alfred L. ; Teffera, Yohannes ; Esteban, Nora V. ; Abramson, Fred P. / Direct determination of human urinary cortisol metabolites by HPLC CRIMS. In: Steroids. 1995 ; Vol. 60, No. 3. pp. 295-298.
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