Diocleinae lectins are a group of proteins with conserved binding sites for the core trimannoside of asparagine-linked oligosaccharides and differential specificities for complex carbohydrates

Tarun K. Dam, Benildo S. Cavada, Thalles B. Grangeiro, Claudia F. Santos, Flavia A M De Sousa, Stefan Oscarson, Curtis F. Brewer

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Abstract

The seed lectin from Dioclea grandiflora and jack bean lectin concanavalin A (ConA) are both members of the Diocleinae subtribe of Leguminosae lectins. Both lectins have recently been shown to possess enhanced affinities and extended binding sites for the trisaccharide, 3,6- di,O-(α-D-mannopyranosyl)-D-mannose, which is present in the core region of all asparagine-linked carbohydrates (Gupta, D., Oscarson, S., Raju, S., Stanley, P. Toone, E. J. and Brewer, C, F. (1996) Eur. J. Biochem. 242, 320- 326). In the present study, the binding specificities of seven other lectins from the Diocleinae subtribe have been investigated by hemagglutination inhibition and isothermal titration microcalorimetry (ITC). The lectins are from Canavalia brasiliensis, Canavalia bonariensis, Cratylia floribunda, Dioclea rostrata, Dioclea virgata, Dioclea violacea, and Dioclea guianensis. Hemagglutination inhibition and ITC experiments show that all seven lectins are Man/Glc-specific and have high affinities for the core trimannoside, like ConA and D. grandiflora lectin. All seven lectins also exhibit the same pattern of binding to a series of monodeoxy analogs and a tetradeoxy analog of the trimannoside, similar to that of ConA and D. grandiflora lectin. However, C. bonariensis, C. floribunda, D. rostrata, and D. violacea, like D. grandiflora, show substantially reduced affinities for a biantennary complex carbohydrate with terminal GlcNAc residues, while C. brasiliensis, D. guianensis, and D. virgata, like ConA, exhibit affinities for the oligosaccharide comparable with that of the trimannoside. Thermodynamic data obtained by ITC indicate different energetic mechanisms of binding of the above two groups of lectins to the complex carbohydrate. The ability of the lectins to induce histamine release from rat peritoneal mast cells is shown to correlate with the relative affinities of the proteins for the biantennary carbohydrate.

Original languageEnglish (US)
Pages (from-to)12082-12088
Number of pages7
JournalJournal of Biological Chemistry
Volume273
Issue number20
DOIs
StatePublished - May 15 1998

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Asparagine
Oligosaccharides
Lectins
Binding Sites
Carbohydrates
Dioclea
Concanavalin A
Proteins
Titration
Hemagglutination
Canavalia
Trisaccharides
Jacks
Histamine Release
Mannose
Thermodynamics
Mast Cells
Fabaceae
Histamine
Seed

ASJC Scopus subject areas

  • Biochemistry

Cite this

Diocleinae lectins are a group of proteins with conserved binding sites for the core trimannoside of asparagine-linked oligosaccharides and differential specificities for complex carbohydrates. / Dam, Tarun K.; Cavada, Benildo S.; Grangeiro, Thalles B.; Santos, Claudia F.; De Sousa, Flavia A M; Oscarson, Stefan; Brewer, Curtis F.

In: Journal of Biological Chemistry, Vol. 273, No. 20, 15.05.1998, p. 12082-12088.

Research output: Contribution to journalArticle

Dam, Tarun K. ; Cavada, Benildo S. ; Grangeiro, Thalles B. ; Santos, Claudia F. ; De Sousa, Flavia A M ; Oscarson, Stefan ; Brewer, Curtis F. / Diocleinae lectins are a group of proteins with conserved binding sites for the core trimannoside of asparagine-linked oligosaccharides and differential specificities for complex carbohydrates. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 20. pp. 12082-12088.
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