Diminished production of monocyte proinflammatoiy cytokines during human immunodeficiency virus viremia is mediated by type I interferons

John C. Tilton, Alison J. Johnson, Marlise R. Luskin, Maura M. Manion, Jun Yang, Joseph W. Adelsberger, Richard A. Lempicki, Claire W. Hallahan, Mary McLaughlin, Jo Ann M Mican, Julia A. Metcalf, Christiana Iyasere, Mark Connors

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

The effect of human immunodeficiency virus (HIV) infection and high-level HIV replication on the function of monocytes was investigated. HIV-positive patients had elevated levels of spontaneous production of some or all of the monocyte proinflammatory cytokines measured (interleukin-1β [IL-1β], IL-6, and tumor necrosis factor alpha [TNF-α]) compared to uninfected controls. In patients on therapy with high frequencies of monocytes producing proinflammatory cytokines, this frequency was diminished in the contest of viremia during an interruption of therapy. Diminished production of proinflammatory cytokines during viremia was restored by culture with autologous CD4+ T cells or monocytes from an on-therapy time point or lipopolysaccharide (LPS). Microarray analysis demonstrated that diminished monocyte production of proinflammatory cytokines was correlated with elevated type I interferon-stimulated gene transcripts. The addition of exogenous alpha 2A Interferon diminished the spontaneous production of IL-1β, IL-6, and TNF-α but did not affect responses to LPS, recapitulating the changes observed for HIV-viremic patients. These results suggest that monocyte function is diminished during high-level HIV viremia and that this effect is mediated by chronic stimulation by type I interferons. This effect on monocytes during viremia may play a role in diminished innate or adaptive immune system functions in HIV-infected patients. In addition, the restoration of these functions may also play a role in some immune reconstitution syndromes observed during initiation of therapy.

Original languageEnglish (US)
Pages (from-to)11486-11497
Number of pages12
JournalJournal of Virology
Volume80
Issue number23
DOIs
StatePublished - Dec 2006
Externally publishedYes

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Interferon Type I
Viremia
viremia
Human immunodeficiency virus
interferons
monocytes
Monocytes
cytokines
HIV
Cytokines
therapeutics
interleukin-1
Interleukin-1
interleukin-6
lipopolysaccharides
tumor necrosis factor-alpha
Lipopolysaccharides
Interleukin-6
Tumor Necrosis Factor-alpha
Immune Reconstitution Inflammatory Syndrome

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Tilton, J. C., Johnson, A. J., Luskin, M. R., Manion, M. M., Yang, J., Adelsberger, J. W., ... Connors, M. (2006). Diminished production of monocyte proinflammatoiy cytokines during human immunodeficiency virus viremia is mediated by type I interferons. Journal of Virology, 80(23), 11486-11497. https://doi.org/10.1128/JVI.00324-06

Diminished production of monocyte proinflammatoiy cytokines during human immunodeficiency virus viremia is mediated by type I interferons. / Tilton, John C.; Johnson, Alison J.; Luskin, Marlise R.; Manion, Maura M.; Yang, Jun; Adelsberger, Joseph W.; Lempicki, Richard A.; Hallahan, Claire W.; McLaughlin, Mary; Mican, Jo Ann M; Metcalf, Julia A.; Iyasere, Christiana; Connors, Mark.

In: Journal of Virology, Vol. 80, No. 23, 12.2006, p. 11486-11497.

Research output: Contribution to journalArticle

Tilton, JC, Johnson, AJ, Luskin, MR, Manion, MM, Yang, J, Adelsberger, JW, Lempicki, RA, Hallahan, CW, McLaughlin, M, Mican, JAM, Metcalf, JA, Iyasere, C & Connors, M 2006, 'Diminished production of monocyte proinflammatoiy cytokines during human immunodeficiency virus viremia is mediated by type I interferons', Journal of Virology, vol. 80, no. 23, pp. 11486-11497. https://doi.org/10.1128/JVI.00324-06
Tilton, John C. ; Johnson, Alison J. ; Luskin, Marlise R. ; Manion, Maura M. ; Yang, Jun ; Adelsberger, Joseph W. ; Lempicki, Richard A. ; Hallahan, Claire W. ; McLaughlin, Mary ; Mican, Jo Ann M ; Metcalf, Julia A. ; Iyasere, Christiana ; Connors, Mark. / Diminished production of monocyte proinflammatoiy cytokines during human immunodeficiency virus viremia is mediated by type I interferons. In: Journal of Virology. 2006 ; Vol. 80, No. 23. pp. 11486-11497.
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abstract = "The effect of human immunodeficiency virus (HIV) infection and high-level HIV replication on the function of monocytes was investigated. HIV-positive patients had elevated levels of spontaneous production of some or all of the monocyte proinflammatory cytokines measured (interleukin-1β [IL-1β], IL-6, and tumor necrosis factor alpha [TNF-α]) compared to uninfected controls. In patients on therapy with high frequencies of monocytes producing proinflammatory cytokines, this frequency was diminished in the contest of viremia during an interruption of therapy. Diminished production of proinflammatory cytokines during viremia was restored by culture with autologous CD4+ T cells or monocytes from an on-therapy time point or lipopolysaccharide (LPS). Microarray analysis demonstrated that diminished monocyte production of proinflammatory cytokines was correlated with elevated type I interferon-stimulated gene transcripts. The addition of exogenous alpha 2A Interferon diminished the spontaneous production of IL-1β, IL-6, and TNF-α but did not affect responses to LPS, recapitulating the changes observed for HIV-viremic patients. These results suggest that monocyte function is diminished during high-level HIV viremia and that this effect is mediated by chronic stimulation by type I interferons. This effect on monocytes during viremia may play a role in diminished innate or adaptive immune system functions in HIV-infected patients. In addition, the restoration of these functions may also play a role in some immune reconstitution syndromes observed during initiation of therapy.",
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