Diminished neurogenic but not pharmacological erections in the 2- to 3- month experimentally diabetic F-344 rat

Jamil Rehman, Eric Chenven, Peter Brink, Beth Peterson, Benjamin Walcott, Yan Ping Wen, Arnold Melman, George Christ

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

The rapid spread of locally restricted neural and hormonal signals among the vast array of largely inexcitable corporal smooth muscle cells is an absolute prerequisite to normal erectile function. And yet the mechanism(s) responsible for this phenomenon is not well understood. As a first step toward a more integrative understanding of erectlie physiology and/or dysfunction, an 8- to 12-wk period of experimental diabetes was induced in 2- mo-old male Fischer 344 rats by either intraperitoneal streptozotocin (STZ) injection (35 mg/kg; n = 22) or subtotal pancreatectomy (n = 11). Fourteen age-matched control animals received injection of vehicle only while nine others served as sham-operated control animals. Eight STZ-diabetic animals received insulin replacement. Erectile function was assessed by evaluation of penile reflexes and monitoring of intracavernous pressure responses to both electrical stimulation of the cavernous nerve and intracorporal papaverine or nitroglycerin injection. Intracavernous pressure responses to neurostimulation were significantly attenuated in both STZ-diabetic and subtotal pancreatectomy animals compared with age-matched control animals (P < 0.05). Penile reflexes were also significantly diminished (P < 0.05). Regression analysis revealed that diabetes-related decreases in neurostimulated intracavernous pressure responses were strongly correlated with diminished synaptophysin immunoreactivity in the corpora (P < 0.001; r = 0.88). However, there were no detectable diabetes-related differences in pharmacological erections induced by intracavernous papaverine or nitroglycerin injection. Northern analysis revealed a marked diabetes- related increase in the amount of connexin 43 mRNA measured in frozen corporal tissue. Insulin replacement partially restored (attenuated the loss of) synaptophysin immunoreactivity and maintained neurostimulated intracavernous pressure responses to control levels while having no effect on penile reflexes. These observations may have important implications to the understanding of erectile physiology as well as the etiology of diabetes- related erectile dysfunction.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume272
Issue number4 41-4
StatePublished - 1997

Fingerprint

Pharmacology
Streptozocin
Reflex
Pressure
Papaverine
Synaptophysin
Pancreatectomy
Nitroglycerin
Injections
Insulin
Connexin 43
Inbred F344 Rats
Erectile Dysfunction
Intraperitoneal Injections
Electric Stimulation
Smooth Muscle Myocytes
Regression Analysis
Messenger RNA

Keywords

  • autonomic neuropathy
  • diabetes
  • erectile dysfunction
  • Fischer 344 rat
  • innervation density
  • intracavernous pressure
  • partial pancreatectomy
  • penile reflexes
  • streptozotocin
  • synpatophysin

ASJC Scopus subject areas

  • Physiology

Cite this

Diminished neurogenic but not pharmacological erections in the 2- to 3- month experimentally diabetic F-344 rat. / Rehman, Jamil; Chenven, Eric; Brink, Peter; Peterson, Beth; Walcott, Benjamin; Wen, Yan Ping; Melman, Arnold; Christ, George.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 272, No. 4 41-4, 1997.

Research output: Contribution to journalArticle

Rehman, J, Chenven, E, Brink, P, Peterson, B, Walcott, B, Wen, YP, Melman, A & Christ, G 1997, 'Diminished neurogenic but not pharmacological erections in the 2- to 3- month experimentally diabetic F-344 rat', American Journal of Physiology - Heart and Circulatory Physiology, vol. 272, no. 4 41-4.
Rehman, Jamil ; Chenven, Eric ; Brink, Peter ; Peterson, Beth ; Walcott, Benjamin ; Wen, Yan Ping ; Melman, Arnold ; Christ, George. / Diminished neurogenic but not pharmacological erections in the 2- to 3- month experimentally diabetic F-344 rat. In: American Journal of Physiology - Heart and Circulatory Physiology. 1997 ; Vol. 272, No. 4 41-4.
@article{4a9a62d4c05f491dae9c33bf1e652b15,
title = "Diminished neurogenic but not pharmacological erections in the 2- to 3- month experimentally diabetic F-344 rat",
abstract = "The rapid spread of locally restricted neural and hormonal signals among the vast array of largely inexcitable corporal smooth muscle cells is an absolute prerequisite to normal erectile function. And yet the mechanism(s) responsible for this phenomenon is not well understood. As a first step toward a more integrative understanding of erectlie physiology and/or dysfunction, an 8- to 12-wk period of experimental diabetes was induced in 2- mo-old male Fischer 344 rats by either intraperitoneal streptozotocin (STZ) injection (35 mg/kg; n = 22) or subtotal pancreatectomy (n = 11). Fourteen age-matched control animals received injection of vehicle only while nine others served as sham-operated control animals. Eight STZ-diabetic animals received insulin replacement. Erectile function was assessed by evaluation of penile reflexes and monitoring of intracavernous pressure responses to both electrical stimulation of the cavernous nerve and intracorporal papaverine or nitroglycerin injection. Intracavernous pressure responses to neurostimulation were significantly attenuated in both STZ-diabetic and subtotal pancreatectomy animals compared with age-matched control animals (P < 0.05). Penile reflexes were also significantly diminished (P < 0.05). Regression analysis revealed that diabetes-related decreases in neurostimulated intracavernous pressure responses were strongly correlated with diminished synaptophysin immunoreactivity in the corpora (P < 0.001; r = 0.88). However, there were no detectable diabetes-related differences in pharmacological erections induced by intracavernous papaverine or nitroglycerin injection. Northern analysis revealed a marked diabetes- related increase in the amount of connexin 43 mRNA measured in frozen corporal tissue. Insulin replacement partially restored (attenuated the loss of) synaptophysin immunoreactivity and maintained neurostimulated intracavernous pressure responses to control levels while having no effect on penile reflexes. These observations may have important implications to the understanding of erectile physiology as well as the etiology of diabetes- related erectile dysfunction.",
keywords = "autonomic neuropathy, diabetes, erectile dysfunction, Fischer 344 rat, innervation density, intracavernous pressure, partial pancreatectomy, penile reflexes, streptozotocin, synpatophysin",
author = "Jamil Rehman and Eric Chenven and Peter Brink and Beth Peterson and Benjamin Walcott and Wen, {Yan Ping} and Arnold Melman and George Christ",
year = "1997",
language = "English (US)",
volume = "272",
journal = "American Journal of Physiology - Renal Fluid and Electrolyte Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "4 41-4",

}

TY - JOUR

T1 - Diminished neurogenic but not pharmacological erections in the 2- to 3- month experimentally diabetic F-344 rat

AU - Rehman, Jamil

AU - Chenven, Eric

AU - Brink, Peter

AU - Peterson, Beth

AU - Walcott, Benjamin

AU - Wen, Yan Ping

AU - Melman, Arnold

AU - Christ, George

PY - 1997

Y1 - 1997

N2 - The rapid spread of locally restricted neural and hormonal signals among the vast array of largely inexcitable corporal smooth muscle cells is an absolute prerequisite to normal erectile function. And yet the mechanism(s) responsible for this phenomenon is not well understood. As a first step toward a more integrative understanding of erectlie physiology and/or dysfunction, an 8- to 12-wk period of experimental diabetes was induced in 2- mo-old male Fischer 344 rats by either intraperitoneal streptozotocin (STZ) injection (35 mg/kg; n = 22) or subtotal pancreatectomy (n = 11). Fourteen age-matched control animals received injection of vehicle only while nine others served as sham-operated control animals. Eight STZ-diabetic animals received insulin replacement. Erectile function was assessed by evaluation of penile reflexes and monitoring of intracavernous pressure responses to both electrical stimulation of the cavernous nerve and intracorporal papaverine or nitroglycerin injection. Intracavernous pressure responses to neurostimulation were significantly attenuated in both STZ-diabetic and subtotal pancreatectomy animals compared with age-matched control animals (P < 0.05). Penile reflexes were also significantly diminished (P < 0.05). Regression analysis revealed that diabetes-related decreases in neurostimulated intracavernous pressure responses were strongly correlated with diminished synaptophysin immunoreactivity in the corpora (P < 0.001; r = 0.88). However, there were no detectable diabetes-related differences in pharmacological erections induced by intracavernous papaverine or nitroglycerin injection. Northern analysis revealed a marked diabetes- related increase in the amount of connexin 43 mRNA measured in frozen corporal tissue. Insulin replacement partially restored (attenuated the loss of) synaptophysin immunoreactivity and maintained neurostimulated intracavernous pressure responses to control levels while having no effect on penile reflexes. These observations may have important implications to the understanding of erectile physiology as well as the etiology of diabetes- related erectile dysfunction.

AB - The rapid spread of locally restricted neural and hormonal signals among the vast array of largely inexcitable corporal smooth muscle cells is an absolute prerequisite to normal erectile function. And yet the mechanism(s) responsible for this phenomenon is not well understood. As a first step toward a more integrative understanding of erectlie physiology and/or dysfunction, an 8- to 12-wk period of experimental diabetes was induced in 2- mo-old male Fischer 344 rats by either intraperitoneal streptozotocin (STZ) injection (35 mg/kg; n = 22) or subtotal pancreatectomy (n = 11). Fourteen age-matched control animals received injection of vehicle only while nine others served as sham-operated control animals. Eight STZ-diabetic animals received insulin replacement. Erectile function was assessed by evaluation of penile reflexes and monitoring of intracavernous pressure responses to both electrical stimulation of the cavernous nerve and intracorporal papaverine or nitroglycerin injection. Intracavernous pressure responses to neurostimulation were significantly attenuated in both STZ-diabetic and subtotal pancreatectomy animals compared with age-matched control animals (P < 0.05). Penile reflexes were also significantly diminished (P < 0.05). Regression analysis revealed that diabetes-related decreases in neurostimulated intracavernous pressure responses were strongly correlated with diminished synaptophysin immunoreactivity in the corpora (P < 0.001; r = 0.88). However, there were no detectable diabetes-related differences in pharmacological erections induced by intracavernous papaverine or nitroglycerin injection. Northern analysis revealed a marked diabetes- related increase in the amount of connexin 43 mRNA measured in frozen corporal tissue. Insulin replacement partially restored (attenuated the loss of) synaptophysin immunoreactivity and maintained neurostimulated intracavernous pressure responses to control levels while having no effect on penile reflexes. These observations may have important implications to the understanding of erectile physiology as well as the etiology of diabetes- related erectile dysfunction.

KW - autonomic neuropathy

KW - diabetes

KW - erectile dysfunction

KW - Fischer 344 rat

KW - innervation density

KW - intracavernous pressure

KW - partial pancreatectomy

KW - penile reflexes

KW - streptozotocin

KW - synpatophysin

UR - http://www.scopus.com/inward/record.url?scp=0030938777&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030938777&partnerID=8YFLogxK

M3 - Article

VL - 272

JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

SN - 1931-857X

IS - 4 41-4

ER -