Digital Autofocus Methods for Automated Microscopy

Feimo Shen, Louis Hodgson, Klaus Hahn

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Automatic focusing of microscope images is an essential part of modern high-throughput microscopy. This chapter describes implementation of a robust autofocus system appropriate for using either air or oil immersion objectives in robotic imaging. Both hardware and software algorithms are described, and caveats of using viscous immersion media with multifield scanning are detailed.

Original languageEnglish (US)
Pages (from-to)620-632
Number of pages13
JournalMethods in Enzymology
Volume414
DOIs
StatePublished - 2006
Externally publishedYes

Fingerprint

Immersion
Microscopy
Microscopic examination
Oils
Robotics
Microscopes
Throughput
Scanning
Hardware
Imaging techniques
Air
Software

ASJC Scopus subject areas

  • Biochemistry

Cite this

Digital Autofocus Methods for Automated Microscopy. / Shen, Feimo; Hodgson, Louis; Hahn, Klaus.

In: Methods in Enzymology, Vol. 414, 2006, p. 620-632.

Research output: Contribution to journalArticle

Shen, Feimo ; Hodgson, Louis ; Hahn, Klaus. / Digital Autofocus Methods for Automated Microscopy. In: Methods in Enzymology. 2006 ; Vol. 414. pp. 620-632.
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