Digestion of RNA of Chromatin and Nuclear Ribonucleoprotein by Staphylococcal Nuclease

Leonard H. Augenlicht, Mary McCormick, Martin Lipkin

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Abstract

Following a 1-h [3H]uridine pulse of cells of a human colon carcinoma line, 15% of the radioactivity in heterogeneous nuclear RNA associated with both chromatin and nuclear ribonucleoprotein was not digested to acid soluble fragments during a 2-h incubation with staphylococcal nuclease (EC 3.1.4.7). These [3H]uridine-labeled oligonucleotides were approximately 26 nucleotides in length. An RNA containing structure which sedimented no faster than 2 S could be isolated from the digests. Major and minor peptide species, of molecular weights 40 000 and 66 000, respectively, were associated with this structure isolated from either chromatin or nuclear ribonucleoprotein. The results demonstrate that some protein of nuclear ribonucleoprotein is complexed with the transcript while it is still associated with chromatin.

Original languageEnglish (US)
Pages (from-to)3818-3823
Number of pages6
JournalBiochemistry
Volume15
Issue number17
DOIs
Publication statusPublished - Aug 1 1976
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry

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