Differentiation of human embryonic stem cells to hepatocytes using deleted variant of HGF and poly-amino-urethane-coated nonwoven polytetrafluoroethylene fabric

Alejandro Soto-Gutierrez, Nalú Navarro-Alvarez, Jorge David Rivas-Carrillo, Yong Chen, Tomoki Yamatsuji, Noriaki Tanaka, Naoya Kobayashi

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Human embryonic stem (hES) cells have recently been studied as an attractive source for the development of a bioartificial liver (BAL). Here we evaluate the differentiation capacity of hES cells into hepatocytes. hES cells were subjected to suspension culture for 5 days, and then cultured onto poly-amino-urethane (PAU)-coated, nonwoven polytetrafluoroethylene (PTFE) fabric in the presence of fibroblast growth factor-2 (bFGF) (100 ng/ml) for 3 days, then with deleted variant of hepatocyte growth factor (dHGF) (100 ng/ml) and 1% dimethyl sulfoxide (DMSO) for 8 days, and finally with dexamethasone (10 -7 M) for 3 days. The hES cells showed gene expression of albumin in a time-dependent manner of the hepatic differentiation process. The resultant hES-derived hepatocytes metabolized the loaded ammonia and lidocaine at 7.8% and 23.6%, respectively. A million of such hepatocytes produced albumin and urea at 351.2 ng and urea at 7.0 μg. Scanning electron microscopy showed good attachment of the cells on the surface of the PTFE fabric and well-developed glycogen rosettes and Gap junction. In the present work we have demonstrated the efficient differentiation of hES cells to functional hepatocytes. The findings are useful to develop a BAL.

Original languageEnglish (US)
Pages (from-to)335-341
Number of pages7
JournalCell Transplantation
Volume15
Issue number4
DOIs
StatePublished - 2006
Externally publishedYes

Fingerprint

Urethane
Polytetrafluoroethylene
Stem cells
Polytetrafluoroethylenes
Hepatocytes
Artificial Liver
Urea
Liver
Albumins
Hepatocyte Growth Factor
Dimethyl sulfoxide
Gap Junctions
Fibroblast Growth Factor 2
Fibroblasts
Lidocaine
Dimethyl Sulfoxide
Glycogen
Cell culture
Ammonia
Gene expression

Keywords

  • Differentiation
  • Hepatocyte
  • Hepatocyte growth factor
  • Human ES cells

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation

Cite this

Differentiation of human embryonic stem cells to hepatocytes using deleted variant of HGF and poly-amino-urethane-coated nonwoven polytetrafluoroethylene fabric. / Soto-Gutierrez, Alejandro; Navarro-Alvarez, Nalú; Rivas-Carrillo, Jorge David; Chen, Yong; Yamatsuji, Tomoki; Tanaka, Noriaki; Kobayashi, Naoya.

In: Cell Transplantation, Vol. 15, No. 4, 2006, p. 335-341.

Research output: Contribution to journalArticle

Soto-Gutierrez, A, Navarro-Alvarez, N, Rivas-Carrillo, JD, Chen, Y, Yamatsuji, T, Tanaka, N & Kobayashi, N 2006, 'Differentiation of human embryonic stem cells to hepatocytes using deleted variant of HGF and poly-amino-urethane-coated nonwoven polytetrafluoroethylene fabric', Cell Transplantation, vol. 15, no. 4, pp. 335-341. https://doi.org/10.3727/000000006783981945
Soto-Gutierrez, Alejandro ; Navarro-Alvarez, Nalú ; Rivas-Carrillo, Jorge David ; Chen, Yong ; Yamatsuji, Tomoki ; Tanaka, Noriaki ; Kobayashi, Naoya. / Differentiation of human embryonic stem cells to hepatocytes using deleted variant of HGF and poly-amino-urethane-coated nonwoven polytetrafluoroethylene fabric. In: Cell Transplantation. 2006 ; Vol. 15, No. 4. pp. 335-341.
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