Differentiated 3T3L1 Adipocytes Are Composed of Heterogenous Cell Populations with Distinct Receptor Tyrosine Kinase Signaling Properties

Satoshi Shigematsu, Sarah L. Miller, Jeffrey E. Pessin

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Various studies have demonstrated that the platelet-derived growth factor (PDGF) receptor in adipocytes can activate PI 3-kinase activity without affecting insulin-responsive glucose transporter (GLUT4) translocation. To investigate this phenomenon of receptor signaling specificity, we utilized single cell analysis to determine the cellular distribution and signaling properties of PDGF and insulin in differentiated 3T3L1 adipocytes. The insulin receptor was highly expressed in a large percentage of the cell population (>95%) that also expressed caveolin 2 and GLUT4 with very low levels of the PDGF receptor. In contrast, the PDGF receptor was only expressed in ∼10% of the differentiated 3T3L1 cell population with relatively low levels of the insulin receptor, caveolin 2, and GLUT4. Consistent with this observation, insulin stimulated the phosphorylation of Akt in the caveolin 2- and GLUT4-positive cells, whereas PDGF primarily stimulated Akt phosphorylation in the caveolin 2- and GLUT4-negative cell population. Furthermore, transfection of the PDGF receptor in the insulin receptor-, GLUT4-, and caveolin 2-positive cells resulted in the ability of PDGF to stimulate GLUT4 translocation. These data demonstrate that differentiated 3T3L1 adipocytes are not a homogeneous population of cells, and the lack of PDGF receptor expression in the GLUT4-positive cell population accounts for the inability of the endogenous PDGF receptor to activate GLUT4 translocation.

Original languageEnglish (US)
Pages (from-to)15292-15297
Number of pages6
JournalJournal of Biological Chemistry
Volume276
Issue number18
DOIs
StatePublished - May 4 2001
Externally publishedYes

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Caveolin 2
Platelet-Derived Growth Factor Receptors
Receptor Protein-Tyrosine Kinases
Adipocytes
Cells
Platelet-Derived Growth Factor
Insulin Receptor
Population
Phosphorylation
Glucose Transporter Type 4
Insulin
Single-Cell Analysis
Phosphatidylinositol 3-Kinases
Transfection

ASJC Scopus subject areas

  • Biochemistry

Cite this

Differentiated 3T3L1 Adipocytes Are Composed of Heterogenous Cell Populations with Distinct Receptor Tyrosine Kinase Signaling Properties. / Shigematsu, Satoshi; Miller, Sarah L.; Pessin, Jeffrey E.

In: Journal of Biological Chemistry, Vol. 276, No. 18, 04.05.2001, p. 15292-15297.

Research output: Contribution to journalArticle

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