Differential tissue and subcellular expression of ERM proteins in normal and malignant tissues: Cytoplasmic ezrin expression has prognostic significance for head and neck squamous cell carcinoma

Rashna Madan; Margaret Brandwein-Gensler; Nicolas F. Schlecht; Kristin Elias; Eleanor Gorbovitsky; Thomas J. Belbin; Radma Mahmood; Dwayne Breining; Hong Qian; Geoffrey Childs; Joseph Locker; Richard Smith; Missak Haigentz; Frank Gunn-Moore; Michael B. Prystowsky

doi:10.1002/hed.20435

Differential tissue and subcellular expression of ERM proteins in normal and malignant tissues: Cytoplasmic ezrin expression has prognostic significance for head and neck squamous cell carcinoma

Rashna Madan, Margaret Brandwein-Gensler, Nicolas F. Schlecht, Kristin Elias, Eleanor Gorbovitsky, Thomas J. Belbin, Radma Mahmood, Dwayne Breining, Hong Qian, Geoffrey Childs, Joseph Locker, Richard Smith, Missak Haigentz, Frank Gunn-Moore, Michael B. Prystowsky

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

Background. Members of the ezrin-radixin-moesin (ERM) protein family regulate cellular shape, motility, and proliferation and potentially influence ability to metastasize. We investigated the correlation between ERM subcellular localization and survival in patients with squamous cell carcinoma (SCC). Methods. Tissue microarrays (TMAs) were constructed from paraffin-embedded tissue. TMA sections were evaluated for ERM protein expression immunohistochemically. The results were compared across clinical and histopathologic variables. Results. ERM staining results for 47 patients showed that cytoplasmic ERM expression was prevalent in tumors (>92%). Whereas ezrin and moesin also localized to the membrane, only willin was found in the nucleus of tumors. Multivariable Cox regression analysis demonstrated that strong cytoplasmic ezrin expression was independently associated with poorer survival (p = .04, hazard ratio 1.82) Conclusions. Both level of expression and subcellular localization of ERM proteins may be important indicators of clinical outcome in SCC. This pilot study justifies the need for an expanded validation study of ERM proteins and clinical outcome.

Original language	English (US)
Pages (from-to)	1018-1027
Number of pages	10
Journal	Head and Neck
Volume	28
Issue number	11
DOIs	https://doi.org/10.1002/hed.20435
State	Published - Nov 2006

Keywords

Ezrin
Head and neck neoplasms
Immunohistochemistry
Moesin
Survival analysis

ASJC Scopus subject areas

Otorhinolaryngology

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10.1002/hed.20435

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Madan, R., Brandwein-Gensler, M., Schlecht, N. F., Elias, K., Gorbovitsky, E., Belbin, T. J., Mahmood, R., Breining, D., Qian, H., Childs, G., Locker, J., Smith, R., Haigentz, M., Gunn-Moore, F., & Prystowsky, M. B. (2006). Differential tissue and subcellular expression of ERM proteins in normal and malignant tissues: Cytoplasmic ezrin expression has prognostic significance for head and neck squamous cell carcinoma. Head and Neck, 28(11), 1018-1027. https://doi.org/10.1002/hed.20435

Differential tissue and subcellular expression of ERM proteins in normal and malignant tissues: Cytoplasmic ezrin expression has prognostic significance for head and neck squamous cell carcinoma. / Madan, Rashna; Brandwein-Gensler, Margaret; Schlecht, Nicolas F. et al.
In: Head and Neck, Vol. 28, No. 11, 11.2006, p. 1018-1027.

Research output: Contribution to journal › Article › peer-review

Madan, R, Brandwein-Gensler, M, Schlecht, NF, Elias, K, Gorbovitsky, E, Belbin, TJ, Mahmood, R, Breining, D, Qian, H, Childs, G, Locker, J, Smith, R, Haigentz, M, Gunn-Moore, F & Prystowsky, MB 2006, 'Differential tissue and subcellular expression of ERM proteins in normal and malignant tissues: Cytoplasmic ezrin expression has prognostic significance for head and neck squamous cell carcinoma', Head and Neck, vol. 28, no. 11, pp. 1018-1027. https://doi.org/10.1002/hed.20435

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title = "Differential tissue and subcellular expression of ERM proteins in normal and malignant tissues: Cytoplasmic ezrin expression has prognostic significance for head and neck squamous cell carcinoma",

abstract = "Background. Members of the ezrin-radixin-moesin (ERM) protein family regulate cellular shape, motility, and proliferation and potentially influence ability to metastasize. We investigated the correlation between ERM subcellular localization and survival in patients with squamous cell carcinoma (SCC). Methods. Tissue microarrays (TMAs) were constructed from paraffin-embedded tissue. TMA sections were evaluated for ERM protein expression immunohistochemically. The results were compared across clinical and histopathologic variables. Results. ERM staining results for 47 patients showed that cytoplasmic ERM expression was prevalent in tumors (>92%). Whereas ezrin and moesin also localized to the membrane, only willin was found in the nucleus of tumors. Multivariable Cox regression analysis demonstrated that strong cytoplasmic ezrin expression was independently associated with poorer survival (p = .04, hazard ratio 1.82) Conclusions. Both level of expression and subcellular localization of ERM proteins may be important indicators of clinical outcome in SCC. This pilot study justifies the need for an expanded validation study of ERM proteins and clinical outcome.",

keywords = "Ezrin, Head and neck neoplasms, Immunohistochemistry, Moesin, Survival analysis",

author = "Rashna Madan and Margaret Brandwein-Gensler and Schlecht, {Nicolas F.} and Kristin Elias and Eleanor Gorbovitsky and Belbin, {Thomas J.} and Radma Mahmood and Dwayne Breining and Hong Qian and Geoffrey Childs and Joseph Locker and Richard Smith and Missak Haigentz and Frank Gunn-Moore and Prystowsky, {Michael B.}",

year = "2006",

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doi = "10.1002/hed.20435",

language = "English (US)",

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pages = "1018--1027",

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T1 - Differential tissue and subcellular expression of ERM proteins in normal and malignant tissues

T2 - Cytoplasmic ezrin expression has prognostic significance for head and neck squamous cell carcinoma

AU - Madan, Rashna

AU - Brandwein-Gensler, Margaret

AU - Schlecht, Nicolas F.

AU - Elias, Kristin

AU - Gorbovitsky, Eleanor

AU - Belbin, Thomas J.

AU - Mahmood, Radma

AU - Breining, Dwayne

AU - Qian, Hong

AU - Childs, Geoffrey

AU - Locker, Joseph

AU - Smith, Richard

AU - Haigentz, Missak

AU - Gunn-Moore, Frank

AU - Prystowsky, Michael B.

PY - 2006/11

Y1 - 2006/11

N2 - Background. Members of the ezrin-radixin-moesin (ERM) protein family regulate cellular shape, motility, and proliferation and potentially influence ability to metastasize. We investigated the correlation between ERM subcellular localization and survival in patients with squamous cell carcinoma (SCC). Methods. Tissue microarrays (TMAs) were constructed from paraffin-embedded tissue. TMA sections were evaluated for ERM protein expression immunohistochemically. The results were compared across clinical and histopathologic variables. Results. ERM staining results for 47 patients showed that cytoplasmic ERM expression was prevalent in tumors (>92%). Whereas ezrin and moesin also localized to the membrane, only willin was found in the nucleus of tumors. Multivariable Cox regression analysis demonstrated that strong cytoplasmic ezrin expression was independently associated with poorer survival (p = .04, hazard ratio 1.82) Conclusions. Both level of expression and subcellular localization of ERM proteins may be important indicators of clinical outcome in SCC. This pilot study justifies the need for an expanded validation study of ERM proteins and clinical outcome.

AB - Background. Members of the ezrin-radixin-moesin (ERM) protein family regulate cellular shape, motility, and proliferation and potentially influence ability to metastasize. We investigated the correlation between ERM subcellular localization and survival in patients with squamous cell carcinoma (SCC). Methods. Tissue microarrays (TMAs) were constructed from paraffin-embedded tissue. TMA sections were evaluated for ERM protein expression immunohistochemically. The results were compared across clinical and histopathologic variables. Results. ERM staining results for 47 patients showed that cytoplasmic ERM expression was prevalent in tumors (>92%). Whereas ezrin and moesin also localized to the membrane, only willin was found in the nucleus of tumors. Multivariable Cox regression analysis demonstrated that strong cytoplasmic ezrin expression was independently associated with poorer survival (p = .04, hazard ratio 1.82) Conclusions. Both level of expression and subcellular localization of ERM proteins may be important indicators of clinical outcome in SCC. This pilot study justifies the need for an expanded validation study of ERM proteins and clinical outcome.

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KW - Head and neck neoplasms

KW - Immunohistochemistry

KW - Moesin

KW - Survival analysis

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Differential tissue and subcellular expression of ERM proteins in normal and malignant tissues: Cytoplasmic ezrin expression has prognostic significance for head and neck squamous cell carcinoma

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