Differential tissue and subcellular expression of ERM proteins in normal and malignant tissues

Cytoplasmic ezrin expression has prognostic significance for head and neck squamous cell carcinoma

Rashna Madan, Margaret Brandwein-Gensler, Nicolas F. Schlecht, Kristin Elias, Eleanor Gorbovitsky, Thomas J. Belbin, Radma Mahmood, Dwayne Breining, Hong Qian, Geoffrey J. Childs, Joseph Locker, Richard V. Smith, Missak Haigentz, Frank Gunn-Moore, Michael B. Prystowsky

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Background. Members of the ezrin-radixin-moesin (ERM) protein family regulate cellular shape, motility, and proliferation and potentially influence ability to metastasize. We investigated the correlation between ERM subcellular localization and survival in patients with squamous cell carcinoma (SCC). Methods. Tissue microarrays (TMAs) were constructed from paraffin-embedded tissue. TMA sections were evaluated for ERM protein expression immunohistochemically. The results were compared across clinical and histopathologic variables. Results. ERM staining results for 47 patients showed that cytoplasmic ERM expression was prevalent in tumors (>92%). Whereas ezrin and moesin also localized to the membrane, only willin was found in the nucleus of tumors. Multivariable Cox regression analysis demonstrated that strong cytoplasmic ezrin expression was independently associated with poorer survival (p = .04, hazard ratio 1.82) Conclusions. Both level of expression and subcellular localization of ERM proteins may be important indicators of clinical outcome in SCC. This pilot study justifies the need for an expanded validation study of ERM proteins and clinical outcome.

Original languageEnglish (US)
Pages (from-to)1018-1027
Number of pages10
JournalHead and Neck
Volume28
Issue number11
DOIs
StatePublished - Nov 2006

Fingerprint

Proteins
Squamous Cell Carcinoma
moesin
radixin
ezrin
Carcinoma, squamous cell of head and neck
Survival
Validation Studies
Paraffin
Neoplasms
Regression Analysis
Staining and Labeling
Membranes

Keywords

  • Ezrin
  • Head and neck neoplasms
  • Immunohistochemistry
  • Moesin
  • Survival analysis

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Differential tissue and subcellular expression of ERM proteins in normal and malignant tissues : Cytoplasmic ezrin expression has prognostic significance for head and neck squamous cell carcinoma. / Madan, Rashna; Brandwein-Gensler, Margaret; Schlecht, Nicolas F.; Elias, Kristin; Gorbovitsky, Eleanor; Belbin, Thomas J.; Mahmood, Radma; Breining, Dwayne; Qian, Hong; Childs, Geoffrey J.; Locker, Joseph; Smith, Richard V.; Haigentz, Missak; Gunn-Moore, Frank; Prystowsky, Michael B.

In: Head and Neck, Vol. 28, No. 11, 11.2006, p. 1018-1027.

Research output: Contribution to journalArticle

Madan, R, Brandwein-Gensler, M, Schlecht, NF, Elias, K, Gorbovitsky, E, Belbin, TJ, Mahmood, R, Breining, D, Qian, H, Childs, GJ, Locker, J, Smith, RV, Haigentz, M, Gunn-Moore, F & Prystowsky, MB 2006, 'Differential tissue and subcellular expression of ERM proteins in normal and malignant tissues: Cytoplasmic ezrin expression has prognostic significance for head and neck squamous cell carcinoma', Head and Neck, vol. 28, no. 11, pp. 1018-1027. https://doi.org/10.1002/hed.20435
Madan, Rashna ; Brandwein-Gensler, Margaret ; Schlecht, Nicolas F. ; Elias, Kristin ; Gorbovitsky, Eleanor ; Belbin, Thomas J. ; Mahmood, Radma ; Breining, Dwayne ; Qian, Hong ; Childs, Geoffrey J. ; Locker, Joseph ; Smith, Richard V. ; Haigentz, Missak ; Gunn-Moore, Frank ; Prystowsky, Michael B. / Differential tissue and subcellular expression of ERM proteins in normal and malignant tissues : Cytoplasmic ezrin expression has prognostic significance for head and neck squamous cell carcinoma. In: Head and Neck. 2006 ; Vol. 28, No. 11. pp. 1018-1027.
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abstract = "Background. Members of the ezrin-radixin-moesin (ERM) protein family regulate cellular shape, motility, and proliferation and potentially influence ability to metastasize. We investigated the correlation between ERM subcellular localization and survival in patients with squamous cell carcinoma (SCC). Methods. Tissue microarrays (TMAs) were constructed from paraffin-embedded tissue. TMA sections were evaluated for ERM protein expression immunohistochemically. The results were compared across clinical and histopathologic variables. Results. ERM staining results for 47 patients showed that cytoplasmic ERM expression was prevalent in tumors (>92{\%}). Whereas ezrin and moesin also localized to the membrane, only willin was found in the nucleus of tumors. Multivariable Cox regression analysis demonstrated that strong cytoplasmic ezrin expression was independently associated with poorer survival (p = .04, hazard ratio 1.82) Conclusions. Both level of expression and subcellular localization of ERM proteins may be important indicators of clinical outcome in SCC. This pilot study justifies the need for an expanded validation study of ERM proteins and clinical outcome.",
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AU - Schlecht, Nicolas F.

AU - Elias, Kristin

AU - Gorbovitsky, Eleanor

AU - Belbin, Thomas J.

AU - Mahmood, Radma

AU - Breining, Dwayne

AU - Qian, Hong

AU - Childs, Geoffrey J.

AU - Locker, Joseph

AU - Smith, Richard V.

AU - Haigentz, Missak

AU - Gunn-Moore, Frank

AU - Prystowsky, Michael B.

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AB - Background. Members of the ezrin-radixin-moesin (ERM) protein family regulate cellular shape, motility, and proliferation and potentially influence ability to metastasize. We investigated the correlation between ERM subcellular localization and survival in patients with squamous cell carcinoma (SCC). Methods. Tissue microarrays (TMAs) were constructed from paraffin-embedded tissue. TMA sections were evaluated for ERM protein expression immunohistochemically. The results were compared across clinical and histopathologic variables. Results. ERM staining results for 47 patients showed that cytoplasmic ERM expression was prevalent in tumors (>92%). Whereas ezrin and moesin also localized to the membrane, only willin was found in the nucleus of tumors. Multivariable Cox regression analysis demonstrated that strong cytoplasmic ezrin expression was independently associated with poorer survival (p = .04, hazard ratio 1.82) Conclusions. Both level of expression and subcellular localization of ERM proteins may be important indicators of clinical outcome in SCC. This pilot study justifies the need for an expanded validation study of ERM proteins and clinical outcome.

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