Differential synthesis of β-major and β-minor globin proteins in murine erythroleukemia cells is regulated at the transcriptional level

S. Ganguly, A. I. Skoultchi, M. D. Garrick, L. M. Garrick, A. S. Campbell, B. P. Alter

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We compared the transcription and translation of globin genes in three mouse erythroleukemia cell lines under different conditions in which there is differential expression of β-major (βma) and β-minor (βmi). Transcription was measured by pulse labeling of whole cell RNA with [3H]uridine, and translation by labeling whole cell protein synthesis with [3H]leucine. Induction with dimethyl sulfoxide led to increased transcription of α, βma, and βmi genes, and the proportion of βmi RNA synthesized was similar to the proportion of βmi globin protein produced, whether the cells produced 30-40% βmi (lines 745 and 9M) or >80% βmi (clone 25-66). Induction with hemin did not lead to increased globin gene transcription in any line, although globin protein synthesis did increase. Thus, the mechanism of β globin chain induction depends on the inducing agent, and not on the cell line or the type of β globin gene product. The relative proportion of βmi and βma globin chain proteins reflects the relative transcription of the two β genes, whether or not transcription increased following induction.

Original languageEnglish (US)
Pages (from-to)3216-3219
Number of pages4
JournalJournal of Biological Chemistry
Issue number7
Publication statusPublished - Jan 1 1988


ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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