Differential subcellular distribution of PC1, PC2 and furin in bovine adrenal medulla and secretion of PC1 and PC2 from this tissue

R. Kirchmair; C. Egger; P. Gee; R. Hogue-Angeletti; R. Fischer-Colbrie; A. Laslop; H. Winkler

doi:10.1016/0304-3940(92)90252-3

Differential subcellular distribution of PC1, PC2 and furin in bovine adrenal medulla and secretion of PC1 and PC2 from this tissue

R. Kirchmair, C. Egger, P. Gee, R. Hogue-Angeletti, R. Fischer-Colbrie, A. Laslop, H. Winkler

Developmental & Molecular Biology

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

The subcellular distribution of PC1, PC2 and furin was determined in bovine adrenal medulla by immunoblotting of fractions obtained by density gradient centrifugation. PC1 and PC2 were found to be confined to chromaffin granules whereas furin (C-terminal-peptide) was absent from these organelles. Stimulation of bovine adrenal medulla by carbamoylcholine chloride induced the secretion of PC1 and PC2. The secreted enzymes had the same molecular size as PC1 and PC2 present in chromaffin granules.

Original language	English (US)
Pages (from-to)	143-145
Number of pages	3
Journal	Neuroscience Letters
Volume	143
Issue number	1-2
DOIs	https://doi.org/10.1016/0304-3940(92)90252-3
State	Published - Aug 31 1992

Keywords

Chromaffin granule
Endopeptidase
Secretion

ASJC Scopus subject areas

General Neuroscience

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10.1016/0304-3940(92)90252-3

Cite this

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title = "Differential subcellular distribution of PC1, PC2 and furin in bovine adrenal medulla and secretion of PC1 and PC2 from this tissue",

abstract = "The subcellular distribution of PC1, PC2 and furin was determined in bovine adrenal medulla by immunoblotting of fractions obtained by density gradient centrifugation. PC1 and PC2 were found to be confined to chromaffin granules whereas furin (C-terminal-peptide) was absent from these organelles. Stimulation of bovine adrenal medulla by carbamoylcholine chloride induced the secretion of PC1 and PC2. The secreted enzymes had the same molecular size as PC1 and PC2 present in chromaffin granules.",

keywords = "Chromaffin granule, Endopeptidase, Secretion",

author = "R. Kirchmair and C. Egger and P. Gee and R. Hogue-Angeletti and R. Fischer-Colbrie and A. Laslop and H. Winkler",

note = "Funding Information: This work was supported by the Fonds zur F6rderung ,ter wissenschaftlichen Forschung (Austria), by the Dr. Legerlotz-Stiftung and by funds from NIH (Grant NS 22697, R.H.-A.)",

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doi = "10.1016/0304-3940(92)90252-3",

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T1 - Differential subcellular distribution of PC1, PC2 and furin in bovine adrenal medulla and secretion of PC1 and PC2 from this tissue

AU - Kirchmair, R.

AU - Egger, C.

AU - Gee, P.

AU - Hogue-Angeletti, R.

AU - Fischer-Colbrie, R.

AU - Laslop, A.

AU - Winkler, H.

N1 - Funding Information: This work was supported by the Fonds zur F6rderung ,ter wissenschaftlichen Forschung (Austria), by the Dr. Legerlotz-Stiftung and by funds from NIH (Grant NS 22697, R.H.-A.)

PY - 1992/8/31

Y1 - 1992/8/31

N2 - The subcellular distribution of PC1, PC2 and furin was determined in bovine adrenal medulla by immunoblotting of fractions obtained by density gradient centrifugation. PC1 and PC2 were found to be confined to chromaffin granules whereas furin (C-terminal-peptide) was absent from these organelles. Stimulation of bovine adrenal medulla by carbamoylcholine chloride induced the secretion of PC1 and PC2. The secreted enzymes had the same molecular size as PC1 and PC2 present in chromaffin granules.

AB - The subcellular distribution of PC1, PC2 and furin was determined in bovine adrenal medulla by immunoblotting of fractions obtained by density gradient centrifugation. PC1 and PC2 were found to be confined to chromaffin granules whereas furin (C-terminal-peptide) was absent from these organelles. Stimulation of bovine adrenal medulla by carbamoylcholine chloride induced the secretion of PC1 and PC2. The secreted enzymes had the same molecular size as PC1 and PC2 present in chromaffin granules.

KW - Chromaffin granule

KW - Endopeptidase

KW - Secretion

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Differential subcellular distribution of PC1, PC2 and furin in bovine adrenal medulla and secretion of PC1 and PC2 from this tissue

Abstract

Keywords

ASJC Scopus subject areas

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