Differential response to acrylonitrile toxicity in rat primary astrocytes and microglia

Samuel Caito, Yingchun Yu, Michael Aschner

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Acrylonitrile (ACN) is a widely used chemical in the production of plastics, resins, nitriles, acrylic fibers, synthetic rubber and acrylamide. While acute high level exposures to ACN are known to be lethal, chronic low dose exposures causes glial cell tumors in rats. Recently, these glial tumors have been characterized as microglial in origin. While effects of ACN on astrocytes, the more numerous glial cell, have been investigated, the effects on microglia are unknown. This study was conducted to compare the responses of astrocytes and microglia to ACN treatment in vitro to address differential sensitivities and adaptive responses to this toxic chemical. Cell viability, ACN uptake, lipid peroxidation byproducts (F2-isoprostanes), glutathione (GSH) levels and expression of NF-E2-related factor 2 (Nrf2) were evaluated in primary rat microglia and astrocytes following ACN treatment. Results indicate that microglia are more sensitive to ACN than astrocytes, accumulating less ACN while demonstrating higher F2-isoprostane levels. GSH levels were up-regulated in both cell types, as a protective mechanism against ACN-induced oxidative stress, while Nrf2 levels were only induced in microglia. Our data suggest that microglia and astrocytes exhibit different sensitivities and responses to ACN, which are linked to the intracellular thiol status inherent to each of these cell types.

Original languageEnglish (US)
Pages (from-to)93-99
Number of pages7
JournalNeuroToxicology
Volume37
DOIs
StatePublished - Jul 2013
Externally publishedYes

Fingerprint

Acrylonitrile
Microglia
Astrocytes
Toxicity
Rats
NF-E2-Related Factor 2
F2-Isoprostanes
Nitrile resins
Neuroglia
Tumors
Elastomers
Acrylic Resins
Nitriles
Oxidative stress
Acrylamide
Poisons
Sulfhydryl Compounds
Glioma
Lipid Peroxidation
Plastics

Keywords

  • Acrylonitrile
  • Astrocytes
  • Glutathione
  • Microglia
  • Oxidative stress

ASJC Scopus subject areas

  • Neuroscience(all)
  • Toxicology

Cite this

Differential response to acrylonitrile toxicity in rat primary astrocytes and microglia. / Caito, Samuel; Yu, Yingchun; Aschner, Michael.

In: NeuroToxicology, Vol. 37, 07.2013, p. 93-99.

Research output: Contribution to journalArticle

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