Differential requirements for the Pax6(5a) genes eyegone and twin of eyegone during eye development in Drosophila

Jih Guang Yao, Bonnie M. Weasner, Lan Hsin Wang, Chuen Chuen Jang, Brandon Weasner, Chiou Yang Tang, Claire L. Salzer, Chun Hong Chen, Bruce Hay, Y. Henry Sun, Justin P. Kumar

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

In eye development the tasks of tissue specification and cell proliferation are regulated, in part, by the Pax6 and Pax6(5a) proteins respectively. In vertebrates, Pax6(5a) is generated as an alternately spliced isoform of Pax6. This stands in contrast to the fruit fly, Drosophila melanogaster, which has two Pax6(5a) homologs that are encoded by the eyegone and twin of eyegone genes. In this report we set out to determine the respective contributions that each gene makes to the development of the fly retina. Here we demonstrate that both eyg and toe encode transcriptional repressors, are expressed in identical patterns but at significantly different levels. We further show, through a molecular dissection of both proteins, that Eyg makes differential use of several domains when compared to Toe and that the number of repressor domains also differs between the two Pax6(5a) homologs. We predict that these results will have implications for elucidating the functional differences between closely related members of other Pax subclasses.

Original languageEnglish (US)
Pages (from-to)535-551
Number of pages17
JournalDevelopmental Biology
Volume315
Issue number2
DOIs
StatePublished - Mar 15 2008
Externally publishedYes

Fingerprint

Toes
Diptera
Drosophila
Drosophila melanogaster
Genes
Vertebrates
Retina
Dissection
Fruit
Protein Isoforms
Proteins
Cell Proliferation

Keywords

  • Drosophila
  • Eye
  • Eyegone
  • Pax6(5a)
  • Retina
  • Twin of eyegone

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Yao, J. G., Weasner, B. M., Wang, L. H., Jang, C. C., Weasner, B., Tang, C. Y., ... Kumar, J. P. (2008). Differential requirements for the Pax6(5a) genes eyegone and twin of eyegone during eye development in Drosophila. Developmental Biology, 315(2), 535-551. https://doi.org/10.1016/j.ydbio.2007.12.037

Differential requirements for the Pax6(5a) genes eyegone and twin of eyegone during eye development in Drosophila. / Yao, Jih Guang; Weasner, Bonnie M.; Wang, Lan Hsin; Jang, Chuen Chuen; Weasner, Brandon; Tang, Chiou Yang; Salzer, Claire L.; Chen, Chun Hong; Hay, Bruce; Sun, Y. Henry; Kumar, Justin P.

In: Developmental Biology, Vol. 315, No. 2, 15.03.2008, p. 535-551.

Research output: Contribution to journalArticle

Yao, JG, Weasner, BM, Wang, LH, Jang, CC, Weasner, B, Tang, CY, Salzer, CL, Chen, CH, Hay, B, Sun, YH & Kumar, JP 2008, 'Differential requirements for the Pax6(5a) genes eyegone and twin of eyegone during eye development in Drosophila', Developmental Biology, vol. 315, no. 2, pp. 535-551. https://doi.org/10.1016/j.ydbio.2007.12.037
Yao, Jih Guang ; Weasner, Bonnie M. ; Wang, Lan Hsin ; Jang, Chuen Chuen ; Weasner, Brandon ; Tang, Chiou Yang ; Salzer, Claire L. ; Chen, Chun Hong ; Hay, Bruce ; Sun, Y. Henry ; Kumar, Justin P. / Differential requirements for the Pax6(5a) genes eyegone and twin of eyegone during eye development in Drosophila. In: Developmental Biology. 2008 ; Vol. 315, No. 2. pp. 535-551.
@article{3ee491248a2e434a812eabbe2c49af40,
title = "Differential requirements for the Pax6(5a) genes eyegone and twin of eyegone during eye development in Drosophila",
abstract = "In eye development the tasks of tissue specification and cell proliferation are regulated, in part, by the Pax6 and Pax6(5a) proteins respectively. In vertebrates, Pax6(5a) is generated as an alternately spliced isoform of Pax6. This stands in contrast to the fruit fly, Drosophila melanogaster, which has two Pax6(5a) homologs that are encoded by the eyegone and twin of eyegone genes. In this report we set out to determine the respective contributions that each gene makes to the development of the fly retina. Here we demonstrate that both eyg and toe encode transcriptional repressors, are expressed in identical patterns but at significantly different levels. We further show, through a molecular dissection of both proteins, that Eyg makes differential use of several domains when compared to Toe and that the number of repressor domains also differs between the two Pax6(5a) homologs. We predict that these results will have implications for elucidating the functional differences between closely related members of other Pax subclasses.",
keywords = "Drosophila, Eye, Eyegone, Pax6(5a), Retina, Twin of eyegone",
author = "Yao, {Jih Guang} and Weasner, {Bonnie M.} and Wang, {Lan Hsin} and Jang, {Chuen Chuen} and Brandon Weasner and Tang, {Chiou Yang} and Salzer, {Claire L.} and Chen, {Chun Hong} and Bruce Hay and Sun, {Y. Henry} and Kumar, {Justin P.}",
year = "2008",
month = "3",
day = "15",
doi = "10.1016/j.ydbio.2007.12.037",
language = "English (US)",
volume = "315",
pages = "535--551",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Differential requirements for the Pax6(5a) genes eyegone and twin of eyegone during eye development in Drosophila

AU - Yao, Jih Guang

AU - Weasner, Bonnie M.

AU - Wang, Lan Hsin

AU - Jang, Chuen Chuen

AU - Weasner, Brandon

AU - Tang, Chiou Yang

AU - Salzer, Claire L.

AU - Chen, Chun Hong

AU - Hay, Bruce

AU - Sun, Y. Henry

AU - Kumar, Justin P.

PY - 2008/3/15

Y1 - 2008/3/15

N2 - In eye development the tasks of tissue specification and cell proliferation are regulated, in part, by the Pax6 and Pax6(5a) proteins respectively. In vertebrates, Pax6(5a) is generated as an alternately spliced isoform of Pax6. This stands in contrast to the fruit fly, Drosophila melanogaster, which has two Pax6(5a) homologs that are encoded by the eyegone and twin of eyegone genes. In this report we set out to determine the respective contributions that each gene makes to the development of the fly retina. Here we demonstrate that both eyg and toe encode transcriptional repressors, are expressed in identical patterns but at significantly different levels. We further show, through a molecular dissection of both proteins, that Eyg makes differential use of several domains when compared to Toe and that the number of repressor domains also differs between the two Pax6(5a) homologs. We predict that these results will have implications for elucidating the functional differences between closely related members of other Pax subclasses.

AB - In eye development the tasks of tissue specification and cell proliferation are regulated, in part, by the Pax6 and Pax6(5a) proteins respectively. In vertebrates, Pax6(5a) is generated as an alternately spliced isoform of Pax6. This stands in contrast to the fruit fly, Drosophila melanogaster, which has two Pax6(5a) homologs that are encoded by the eyegone and twin of eyegone genes. In this report we set out to determine the respective contributions that each gene makes to the development of the fly retina. Here we demonstrate that both eyg and toe encode transcriptional repressors, are expressed in identical patterns but at significantly different levels. We further show, through a molecular dissection of both proteins, that Eyg makes differential use of several domains when compared to Toe and that the number of repressor domains also differs between the two Pax6(5a) homologs. We predict that these results will have implications for elucidating the functional differences between closely related members of other Pax subclasses.

KW - Drosophila

KW - Eye

KW - Eyegone

KW - Pax6(5a)

KW - Retina

KW - Twin of eyegone

UR - http://www.scopus.com/inward/record.url?scp=39949083200&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=39949083200&partnerID=8YFLogxK

U2 - 10.1016/j.ydbio.2007.12.037

DO - 10.1016/j.ydbio.2007.12.037

M3 - Article

C2 - 18275947

AN - SCOPUS:39949083200

VL - 315

SP - 535

EP - 551

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 2

ER -