Differential requirements for the Pax6(5a) genes eyegone and twin of eyegone during eye development in Drosophila

Jih Guang Yao, Bonnie M. Weasner, Lan Hsin Wang, Chuen Chuen Jang, Brandon Weasner, Chiou Yang Tang, Claire L. Salzer, Chun Hong Chen, Bruce Hay, Y. Henry Sun, Justin P. Kumar

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

In eye development the tasks of tissue specification and cell proliferation are regulated, in part, by the Pax6 and Pax6(5a) proteins respectively. In vertebrates, Pax6(5a) is generated as an alternately spliced isoform of Pax6. This stands in contrast to the fruit fly, Drosophila melanogaster, which has two Pax6(5a) homologs that are encoded by the eyegone and twin of eyegone genes. In this report we set out to determine the respective contributions that each gene makes to the development of the fly retina. Here we demonstrate that both eyg and toe encode transcriptional repressors, are expressed in identical patterns but at significantly different levels. We further show, through a molecular dissection of both proteins, that Eyg makes differential use of several domains when compared to Toe and that the number of repressor domains also differs between the two Pax6(5a) homologs. We predict that these results will have implications for elucidating the functional differences between closely related members of other Pax subclasses.

Original languageEnglish (US)
Pages (from-to)535-551
Number of pages17
JournalDevelopmental Biology
Volume315
Issue number2
DOIs
StatePublished - Mar 15 2008

Keywords

  • Drosophila
  • Eye
  • Eyegone
  • Pax6(5a)
  • Retina
  • Twin of eyegone

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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    Yao, J. G., Weasner, B. M., Wang, L. H., Jang, C. C., Weasner, B., Tang, C. Y., Salzer, C. L., Chen, C. H., Hay, B., Sun, Y. H., & Kumar, J. P. (2008). Differential requirements for the Pax6(5a) genes eyegone and twin of eyegone during eye development in Drosophila. Developmental Biology, 315(2), 535-551. https://doi.org/10.1016/j.ydbio.2007.12.037