Differential regulation of gonadotropin receptors by bone morphogenetic proteins in the zebrafish ovary

Cheuk Wun Li, Rui Zhou, Wei Ge

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Follicle-stimulating hormone receptor (fshr) and luteinizing hormone/choriogonadotropin receptor (lhcgr) exhibit differential temporal expression patterns during zebrafish folliculogenesis with fshr being dominant during vitellogenic growth and lhcgr increasing its expression dramatically before maturation. The dynamic and distinct expression patterns of fshr and lhcgr suggest that they are under tight regulatory control. However, the underlying mechanisms for the differential expression of the two receptors remain unknown. We have recently demonstrated that members of bone morphogenetic protein (BMP) family are largely expressed in the oocyte, while their receptors are exclusively localized on the follicle cells, suggesting a potential paracrine signaling from the oocyte to the follicle cells by BMPs. In this study, we investigated the effects of zebrafish BMP2b (zfBmp2b) and BMP4 (zfBmp4) on the expression of fshr and lhcgr using a novel co-culture approach. The recombinant zfBmp2b or zfBmp4-producing CHO cells were co-cultured with the zebrafish follicle cells followed by real-time qPCR analysis of fshr and lhcgr expression. Our results showed that zfBmp2b and zfBmp4 both down-regulated fshr, while up-regulated lhcgr expression at 24. h of co-culturing. This finding, together with the high expression level of BMP receptors in the follicle cells prior to oocyte maturation, strongly suggests a potential role for BMPs in the differential expression of fshr and lhcgr, especially in the full-grown follicles before maturation. As BMPs are largely expressed in the oocyte, this also implies an important role for the oocyte in orchestrating the differentiation and function of the follicle cells.

Original languageEnglish (US)
Pages (from-to)420-425
Number of pages6
JournalGeneral and Comparative Endocrinology
Volume176
Issue number3
DOIs
StatePublished - May 1 2012
Externally publishedYes

Fingerprint

Gonadotropin Receptors
FSH Receptors
LH Receptors
bone morphogenetic proteins
Bone Morphogenetic Proteins
hormone receptors
Zebrafish
Chorionic Gonadotropin
luteinizing hormone
Danio rerio
Ovary
Oocytes
oocytes
cells
receptors
Bone Morphogenetic Protein Receptors
Paracrine Communication
CHO Cells
follicular development
coculture

Keywords

  • Bmp
  • Fshr
  • Lhcgr
  • Ovary
  • Zebrafish

ASJC Scopus subject areas

  • Endocrinology

Cite this

Differential regulation of gonadotropin receptors by bone morphogenetic proteins in the zebrafish ovary. / Li, Cheuk Wun; Zhou, Rui; Ge, Wei.

In: General and Comparative Endocrinology, Vol. 176, No. 3, 01.05.2012, p. 420-425.

Research output: Contribution to journalArticle

@article{ab0f5c8f88454ecea580f8e58320389c,
title = "Differential regulation of gonadotropin receptors by bone morphogenetic proteins in the zebrafish ovary",
abstract = "Follicle-stimulating hormone receptor (fshr) and luteinizing hormone/choriogonadotropin receptor (lhcgr) exhibit differential temporal expression patterns during zebrafish folliculogenesis with fshr being dominant during vitellogenic growth and lhcgr increasing its expression dramatically before maturation. The dynamic and distinct expression patterns of fshr and lhcgr suggest that they are under tight regulatory control. However, the underlying mechanisms for the differential expression of the two receptors remain unknown. We have recently demonstrated that members of bone morphogenetic protein (BMP) family are largely expressed in the oocyte, while their receptors are exclusively localized on the follicle cells, suggesting a potential paracrine signaling from the oocyte to the follicle cells by BMPs. In this study, we investigated the effects of zebrafish BMP2b (zfBmp2b) and BMP4 (zfBmp4) on the expression of fshr and lhcgr using a novel co-culture approach. The recombinant zfBmp2b or zfBmp4-producing CHO cells were co-cultured with the zebrafish follicle cells followed by real-time qPCR analysis of fshr and lhcgr expression. Our results showed that zfBmp2b and zfBmp4 both down-regulated fshr, while up-regulated lhcgr expression at 24. h of co-culturing. This finding, together with the high expression level of BMP receptors in the follicle cells prior to oocyte maturation, strongly suggests a potential role for BMPs in the differential expression of fshr and lhcgr, especially in the full-grown follicles before maturation. As BMPs are largely expressed in the oocyte, this also implies an important role for the oocyte in orchestrating the differentiation and function of the follicle cells.",
keywords = "Bmp, Fshr, Lhcgr, Ovary, Zebrafish",
author = "Li, {Cheuk Wun} and Rui Zhou and Wei Ge",
year = "2012",
month = "5",
day = "1",
doi = "10.1016/j.ygcen.2011.12.032",
language = "English (US)",
volume = "176",
pages = "420--425",
journal = "General and Comparative Endocrinology",
issn = "0016-6480",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Differential regulation of gonadotropin receptors by bone morphogenetic proteins in the zebrafish ovary

AU - Li, Cheuk Wun

AU - Zhou, Rui

AU - Ge, Wei

PY - 2012/5/1

Y1 - 2012/5/1

N2 - Follicle-stimulating hormone receptor (fshr) and luteinizing hormone/choriogonadotropin receptor (lhcgr) exhibit differential temporal expression patterns during zebrafish folliculogenesis with fshr being dominant during vitellogenic growth and lhcgr increasing its expression dramatically before maturation. The dynamic and distinct expression patterns of fshr and lhcgr suggest that they are under tight regulatory control. However, the underlying mechanisms for the differential expression of the two receptors remain unknown. We have recently demonstrated that members of bone morphogenetic protein (BMP) family are largely expressed in the oocyte, while their receptors are exclusively localized on the follicle cells, suggesting a potential paracrine signaling from the oocyte to the follicle cells by BMPs. In this study, we investigated the effects of zebrafish BMP2b (zfBmp2b) and BMP4 (zfBmp4) on the expression of fshr and lhcgr using a novel co-culture approach. The recombinant zfBmp2b or zfBmp4-producing CHO cells were co-cultured with the zebrafish follicle cells followed by real-time qPCR analysis of fshr and lhcgr expression. Our results showed that zfBmp2b and zfBmp4 both down-regulated fshr, while up-regulated lhcgr expression at 24. h of co-culturing. This finding, together with the high expression level of BMP receptors in the follicle cells prior to oocyte maturation, strongly suggests a potential role for BMPs in the differential expression of fshr and lhcgr, especially in the full-grown follicles before maturation. As BMPs are largely expressed in the oocyte, this also implies an important role for the oocyte in orchestrating the differentiation and function of the follicle cells.

AB - Follicle-stimulating hormone receptor (fshr) and luteinizing hormone/choriogonadotropin receptor (lhcgr) exhibit differential temporal expression patterns during zebrafish folliculogenesis with fshr being dominant during vitellogenic growth and lhcgr increasing its expression dramatically before maturation. The dynamic and distinct expression patterns of fshr and lhcgr suggest that they are under tight regulatory control. However, the underlying mechanisms for the differential expression of the two receptors remain unknown. We have recently demonstrated that members of bone morphogenetic protein (BMP) family are largely expressed in the oocyte, while their receptors are exclusively localized on the follicle cells, suggesting a potential paracrine signaling from the oocyte to the follicle cells by BMPs. In this study, we investigated the effects of zebrafish BMP2b (zfBmp2b) and BMP4 (zfBmp4) on the expression of fshr and lhcgr using a novel co-culture approach. The recombinant zfBmp2b or zfBmp4-producing CHO cells were co-cultured with the zebrafish follicle cells followed by real-time qPCR analysis of fshr and lhcgr expression. Our results showed that zfBmp2b and zfBmp4 both down-regulated fshr, while up-regulated lhcgr expression at 24. h of co-culturing. This finding, together with the high expression level of BMP receptors in the follicle cells prior to oocyte maturation, strongly suggests a potential role for BMPs in the differential expression of fshr and lhcgr, especially in the full-grown follicles before maturation. As BMPs are largely expressed in the oocyte, this also implies an important role for the oocyte in orchestrating the differentiation and function of the follicle cells.

KW - Bmp

KW - Fshr

KW - Lhcgr

KW - Ovary

KW - Zebrafish

UR - http://www.scopus.com/inward/record.url?scp=84862810212&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862810212&partnerID=8YFLogxK

U2 - 10.1016/j.ygcen.2011.12.032

DO - 10.1016/j.ygcen.2011.12.032

M3 - Article

VL - 176

SP - 420

EP - 425

JO - General and Comparative Endocrinology

JF - General and Comparative Endocrinology

SN - 0016-6480

IS - 3

ER -